Institution
Goethe University Frankfurt
Education•Frankfurt am Main, Hessen, Germany•
About: Goethe University Frankfurt is a education organization based out in Frankfurt am Main, Hessen, Germany. It is known for research contribution in the topics: Population & Transplantation. The organization has 33342 authors who have published 69743 publications receiving 2409538 citations. The organization is also known as: Johann Wolfgang Goethe-Universität Frankfurt am Main & University of Frankfurt am Main.
Topics: Population, Transplantation, Medicine, Context (language use), Cancer
Papers published on a yearly basis
Papers
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TL;DR: High stochasticity in individual PCR reactions and distance decay analysis indicated that community similarity decreased slightly with geographical distance, suggesting that sampling of soil fungal communities is more exhaustive, if the authors combine repeated PCR products, and PCR products generated at various annealing temperatures.
Abstract: Next generation metabarcoding is becoming an indispensable tool in fungal community ecology. Here we tested Illumina metabarcoding, a method that generates shorter reads but achieves deeper sequencing than 454 metabarcoding approaches. We found that paired-end Illumina MiSeq data cover the full ITS1 in many fungal lineages and are suitable for environmental fungal community assessment. There was substantial read loss during data cleanup (78.6%), which, however, did not impede the analyses, because of the large number of initial sequences (over 4Mio). We observed a high stochasticity in individual PCR reactions. Comparing three repeated sets of PCRs products showed that 58.5% of the total fungal operational taxonomic units (OTUs) found were not recovered by any single set of PCR reactions. Similarly, comparing three annealing temperatures showed that 63.6% of all fungal OTUs were not recovered using any single annealing temperature. These findings suggest that sampling of soil fungal communities is more exhaustive, if we combine repeated PCR products, and PCR products generated at various annealing temperatures. To analyze the above issues we sampled 16 soil cores along a 270 cm transect in a meadow. In total we recovered 3320 fungal OTUs (based on a 95% similarity threshold). Distance decay analysis indicated that community similarity decreased slightly with geographical distance.
349 citations
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TL;DR: The data suggest that high potency marijuana consistently impairs executive function and motor control and use of higher doses of THC in controlled studies may offer a reliable indication of THC induced impairment as compared to lower doses that have traditionally been used in performance studies.
349 citations
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TL;DR: The dock–lock mechanism should be a generic mechanism for growth of oligomers of amyloidogenic peptides, and surprisingly, the mobile structured oligomers undergo large conformational changes in order to accommodate the added monomer.
Abstract: Nonfibrillar soluble oligomers, which are intermediates in the transition from monomers to amyloid fibrils, may be the toxic species in Alzheimer's disease. To monitor the early events that direct assembly of amyloidogenic peptides we probe the dynamics of formation of (Abeta(16-22))(n) by adding a monomer to a preformed (Abeta(16-22))(n-1) (n = 4-6) oligomer in which the peptides are arranged in an antiparallel beta-sheet conformation. All atom molecular dynamics simulations in water and multiple long trajectories, for a cumulative time of 6.9 mus, show that the oligomer grows by a two-stage dock-lock mechanism. The largest conformational change in the added disordered monomer occurs during the rapid ( approximately 50 ns) first dock stage in which the beta-strand content of the monomer increases substantially from a low initial value. In the second slow-lock phase, the monomer rearranges to form in register antiparallel structures. Surprisingly, the mobile structured oligomers undergo large conformational changes in order to accommodate the added monomer. The time needed to incorporate the monomer into the fluid-like oligomer grows even when n = 6, which suggests that the critical nucleus size must exceed six. Stable antiparallel structure formation exceeds hundreds of nanoseconds even though frequent interpeptide collisions occur at elevated monomer concentrations used in the simulations. The dock-lock mechanism should be a generic mechanism for growth of oligomers of amyloidogenic peptides.
349 citations
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TL;DR: A group of representatives from breast cancer clinical research groups in France, Germany, Italy, the United Kingdom, and the United States reviewed all available data on prospective randomized trials in this setting to address concern about the use of PST in routine practice.
Abstract: Primary systemic therapy (PST) represents the standard of care in patients with locally advanced breast cancer. In addition, there is increasing information on PST in operable breast disease that supports the use of PST in routine practice. However, current regimens and techniques vary. To address this concern, a group of representatives from breast cancer clinical research groups in France, Germany, Italy, the United Kingdom, and the United States reviewed all available data on prospective randomized trials in this setting. Recommendations are made regarding terminology, indications, regimen, diagnosis before treatment, monitoring of efficacy, tumor localization, surgery, pathologic evaluation, and postoperative treatment.
348 citations
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Potsdam Institute for Climate Impact Research1, University of Maryland, College Park2, Massachusetts Institute of Technology3, Met Office4, Humboldt University of Berlin5, Delft University of Technology6, Japan Agency for Marine-Earth Science and Technology7, University of Washington8, Dalhousie University9, University of Chicago10, Columbia University11, Autonomous University of Barcelona12, Catalan Institution for Research and Advanced Studies13, University of Nottingham14, Catalan Institute for Water Research15, Goethe University Frankfurt16, Utrecht University17, Uppsala University18, United Nations Environment Programme19, Wageningen University and Research Centre20, Durham University21, Auburn University22, Chinese Academy of Sciences23
TL;DR: In Paris, France, December 2015, the Conference of the Parties (COP) to the United Nations Framework Concerning on Climate Change (UNFCCC) invited the Inter- governmental Panel on Climate change (IPCC).
Abstract: In Paris, France, December 2015, the Conference of the Parties (COP) to the United Nations Framework Con- vention on Climate Change (UNFCCC) invited the Inter- governmental Panel on Climate Change ...
348 citations
Authors
Showing all 33782 results
Name | H-index | Papers | Citations |
---|---|---|---|
Jorge E. Cortes | 163 | 2784 | 124154 |
Marc W. Kirschner | 162 | 457 | 102145 |
Klaus Rajewsky | 154 | 504 | 88793 |
Andreas Pfeiffer | 149 | 1756 | 131080 |
Stefanie Dimmeler | 147 | 574 | 81658 |
Hans Peter Beck | 143 | 1134 | 91858 |
Gunther Roland | 141 | 1471 | 100681 |
Ad Bax | 138 | 486 | 97112 |
David G. Harrison | 137 | 492 | 72190 |
Paul Brennan | 132 | 1221 | 72748 |
Andreas M. Zeiher | 129 | 571 | 75125 |
Jürgen Habermas | 126 | 503 | 114175 |
Vincenzo Di Marzo | 126 | 659 | 60240 |
Stuart J. Connolly | 125 | 610 | 75925 |
James D. Griffin | 124 | 490 | 55565 |