Texas Medical Center

About: Texas Medical Center is a healthcare organization based out in Houston, Texas, United States. It is known for research contribution in the topics: Population & Cancer. The organization has 2845 authors who have published 2394 publications receiving 79426 citations.

Effect of Repeated Replenishment of Hypothalamie Extract on LH and FSH Secretion in Monolayer Cultures of Rat Anterior Pituitary Cells1

Abstract: Secretion of LH and FSH in primary monolayer cultures of rat anterior pituitary cells was investigated over a period of 41 days. The effects of rat hypothalamie extract (HE) on cell growth and hormone secretion were assessed. The culture media were replenished at 3–4-day intervals and their LH and FSH contents were measured by double antibody radioimmunoassay methods. In absence of HE, the concentrations of LH and FSH declined gradually and reached undetectable levels (<2S0 ng/culture in terms of standard reference preparation NIAMD—Rat LHRP- 1 and NIAMD—Rat FSH—RP-1, respectively) by the 16th day of cultivation. When the HE was replenished at 3–4-day intervals the concentrations of both gonadotrophins also declined but always remained considerably higher than for control cultures. Addition of HE to 21-day cultures, grown in medium alone, increased the hormone concentrations to 1,100 ng/culture for LH and to 2,500 ng/culture for FSH.(Endocrinology 92: 6, 1973)

Vincristine, prednisone, and daunomycin in acute leukemia of childhood. Writing committee

Abstract: T w o different regimens utilizing daunomycin, vincristine sulfate, and prednisone were administered in combination to treat 64 children with acute leukemia in relapse. All patients had received previous therapy with vincristine, prednisone, and/or daunomycin but none had received the 3 drugs in combination. Seven were known to be resistant to both vincristine and prednisone. The triple combination was effective in inducing remission in 44 of the children, but myelosuppression was severe and the results obtained were not significantly better than the much less toxic combination of vincristine and prednisone.

Radiothyroxine Turnover Studies in Mice: Effect of Temperature, Diet, Sex and Pregnancy

Abstract: The radiothyroxine turnover technique was adapted for measurements of thyroxine secretion rate (T4SR) in individual mice. This is calculated by determining the fractional turnover rate of plasma T4 (K), the thyroxine distribution volume (TDS), and PBI of each animal and assumes that T4 utilization equals T4SR. In one strain of mice, mean values for normal males at 24 C were: t½, 10 hr; K, 6.9%/hr; PBI, 2 μg/100 ml; TDS, 4 ml/animal or 16 ml/100 g body wt; T4SR, 0.8 μg/100 g body wt/day. Large doses of T4 injected throughout the 48-hr experimental period significantly accelerated fractional turnover and decreased TDS. Iodide injections did not alter either of these parameters. After one week on an iodinedeficient diet, the K and T4SR were both significantly reduced as compared to control mice; however, this difference was partially due to recirculation of degraded radiothyroxine in the iodine-deficient mice. Cold exposure for 72 Insignificantly accelerated K and elevated T4SR in male mice. Exposure at 32 C...