Showing papers by "University of Colorado Denver published in 1986"
TL;DR: An electrophoretic analysis of a series of closely related DNA polymers in which oligo(dA)–oligo(dT) runs of different polarity were compared concluded that ApA dinucleotide wedges cannot account for DNA curvature.
Abstract: DNAs from both prokaryotic1–4 and eukaryotic5–8 organisms have yielded restriction fragments which manifest markedly anomalous electrophoretic behaviour (reduced mobility) when run on polyacrylamide gels. We have shown previously9 that the abnormal electrophoretic behaviour of one such fragment is a consequence of stable curvature of the helix axis in solution. The molecules involved tend to contain oligo(dA)–oligo(dT) runs which are approximately in-phase with the helix repeat7,8,10; however, the precise structural elements responsible for DNA curvature have not been identified. One popular model11,12 for curvature invokes a non-coplanar ‘wedge-like’ conformation of ApA/TpT dinucleotide pairs. Despite a lack of direct evidence in support of this model, it has been used to provide quantitative estimates of curvature4,13,14. To critically evaluate the ApA wedge model, we have performed an electrophoretic analysis of a series of closely related DNA polymers in which oligo(dA)–oligo(dT) runs of different polarity were compared. We conclude that ApA dinucleotide wedges cannot account for DNA curvature. Therefore, quantitative estimates for ApA wedge deformations, based solely on apparent curvature, cannot be correct.
358 citations
TL;DR: The demonstrated difference between the LS and SS mice in muscimol stimulated chloride uptake as well as in musCimol displacement of [35S]TBPS binding offers a biochemical explanation for the line differences in behavioral responses to GABAergic agents.
274 citations
TL;DR: The pathogenesis of P. aeruginosa infections is multifactorial, as manifested by the numerous toxins, or virulence factors, it produces and the variety of diseases it causes.
167 citations
TL;DR: In this paper, a trend test for evidence of a dose response is proposed for such SCE data, where the percent of cells with chromosome aberrations is the response of interest, and Monte Carlo methods are used to show that the trend test is more sensitive than four other statistical procedures considered for the analysis of Poisson-distributed SCE.
Abstract: It is a widely held view that objective statistical criteria are needed for the evaluation of genetic toxicity assays. This paper presents statistical methods for the analysis of data from in vitro sister chromatid exchange (SCE) and chromosome aberration tests that use Chinese hamster ovary cells. For SCEs, an extensive study of solvent control results demonstrated that there is a substantial interday component of variability in the data, and that a Poisson sampling model is applicable to data generated via the protocol of Galloway et al [1985]. Consequently, a trend test for evidence of a dose response is proposed for such SCE data. As an illustration of this statistical method, analysis of data previously considered to be negative [Gulati et al, 1985] indicates that di(2-ethyl-hexyl) phthalate induces a weak, but reproducible, SCE dose response in CHO cells. Monte Carlo methods are used to show that the trend test is more sensitive than four other statistical procedures considered for the analysis of Poisson-distributed SCEs. A similar trend test for dose response in proportions is proposed for chromosome aberration data, where the percent of cells with chromosome aberrations is the response of interest. Sensitivity (or power) studies indicate that three doses and a control with 50 cells/dose point is a reasonable design for an in vitro SCE study that uses the Galloway et al protocol. For in vitro chromosome aberrations, however, three doses and a control with 100 cells/dose point appears to produce too insensitive an assay; an increase to 200 cells/dose point in the Galloway et al protocol seems worthy of serious consideration.
157 citations
TL;DR: Stone-play has been observed among the free-ranging Arashiyama B troop Japanese macaques near Kyoto, Japan since 1979 as mentioned in this paper, and the progression of this behavior is traced through three phases: transmission, tradition, and transformation.
Abstract: Stone-play, a newly innovated cultural behavior, has been observed among the free-ranging Arashiyama B troop Japanese macaques near Kyoto, Japan since 1979. Conditions in which the non-purposeful handling of stones might possibly give rise to tool behavior are discussed. The progression of this behavior is traced through three phases: transmission, tradition, and transformation. During the first two phases, through social learning, the behavior was established within the group as a regular item of their behavioral repertoire and was most frequently observed after eating provisioned grain. In the third phase, observations suggest a “faddish” shift in the practice of certain behavioral sub-types between 1984 and 1985. During this period young individuals increasingly began to carry stones away from the feeding station, mixing stone manipulation with forage-feeding activities in the forest. Observations suggest under such conditions, stone handling is likely to lead to the occasional use of stone as a tool. This conclusion probably can be applied to species other thanMacaca fuscata. Consideration of the eco-setting and social learning correlates of stone handling suggests how the instrumental use of stone might emerge from a tradition of non-instrumental manipulation.
151 citations
TL;DR: Following 5/6 nephrectomy, 18 rats were fed a normal diet and when serum phosphorus (SPi) and serum calcium (SCa2+) were normal, the rats were separated into two groups, matched and paired by body weight and SCr, and housed separately in metabolic cages.
144 citations
TL;DR: It is concluded that Hansel's stain substantially improves the recognition of eosinophiluria as compared with Wright's stain, which is useful in distinguishing acute interstitial nephritis from acute tubular necrosis.
Abstract: Eosinophiluria is considered a useful marker of drug-induced acute interstitial nephritis. However, recognition of eosinophiluria by Wright's staining is technically difficult, and the spectrum of disorders causing eosinophiluria is not completely defined. We have adapted Hansel's stain for the examination of urinary sediment. Whereas there was a variable uptake of Wright's stain by eosinophils in the urine, such eosinophils were readily recognized with Hansel's stain by the presence of bright red granules. The prevalence of eosinophiluria in acute interstitial nephritis was 10 of 11 patients, in acute tubular necrosis none of 30, in acute pyelonephritis none of 10, in acute cystitis 1 of 15, in postinfectious glomerulonephritis 1 of 6, in rapidly progressive glomerulonephritis 4 of 10, and in acute prostatitis 6 of 10. Eosinophiluria in acute interstitial nephritis was demonstrated by Hansel's stain in 10 of 11 patients but by Wright's stain in only 2 of 11 patients. We conclude that Hansel's stain substantially improves the recognition of eosinophiluria as compared with Wright's stain. Eosinophiluria is useful in distinguishing acute interstitial nephritis from acute tubular necrosis. The clinical spectrum of eosinophiluria also includes rapidly progressive glomerulonephritis, acute prostatitis, and occasionally, acute cystitis or postinfectious glomerulonephritis.
140 citations
TL;DR: This case-control study was designed to investigate the relation between reproductive history and occurrence of hip and forearm fractures in postmenopausal women and found that women who gave birth four or more times had a risk of fracture similar to women who had not given birth.
Abstract: This case-control study was designed to investigate the relation between reproductive history and occurrence of hip and forearm fractures in postmenopausal women. Three hundred and fifty-five King County, Washington women who sustained a fracture between 1976 and 1980 and 562 control women were interviewed regarding their reproductive history and other factors. After controlling for the confounding effects of age, obesity, and use of noncontraceptive estrogens, the authors found that women who gave birth four or more times had a risk of fracture similar to women who had not given birth (odds ratio = 1.2, 95% confidence interval = 0.7-2.2), and women who breastfed for more than two years had a risk of fracture similar to women who had never breastfed (odds ratio = 0.8, 95% confidence interval = 0.4-1.5).
126 citations
TL;DR: expression of the core region of the hepatitis B virus genome in mammalian cells with recombinant plasmid vectors shows that partial deletion of the precore sequences abolished secretion of the e antigen, but there was pronounced synthesis of thecore antigen in transfected cells.
Abstract: We studied the expression of the core region of the hepatitis B virus genome in mammalian cells with recombinant plasmid vectors. Stably transformed rat fibroblast cell lines were established by transfection with vectors containing subgenomic and genome-length hepatitis B virus DNA, followed by G418 selection. The RNA transcripts directed by the core region were characterized by Northern blot hybridization and S1 nuclease mapping. Using the chloramphenicol acetyltransferase gene expression system, the promoter activity located upstream of the core open reading frame was confirmed. The synthesis of core and e polypeptides was studied with a commercial radioimmunoassay. These studies show that partial deletion of the precore sequences abolished secretion of the e antigen, but there was pronounced synthesis of the core antigen in transfected cells.
125 citations
TL;DR: Cultured PKD epithelia provide a new tool for the study of the pathogenesis of this disease and suggest a defect in cell growth and basement membrane synthesis in human PKD.
125 citations
TL;DR: In the case of the Purgatoire River tracks, the trackway orientations and footprint-depth contours pinpoint the location of the paleoshoreline at successive levels in the section as discussed by the authors.
Abstract: Little-known exposures of the Upper Jurassic Morrison Formation from the Purgatoire Valley of southeastern Colorado have yielded the world's largest continuously mapped assemblage of dinosaur trackways. Body fossils include plant, invertebrate, and vertebrate remains indicative of predominantly fresh-water conditions. The tracks occur in a lacustrine sequence characterized by (1) shallow-water shales; (2) micritic shoreface limestones with ooids, intraclasts, ripple marks, mud cracks; and (3) minor quartzose sandstone with salt-crystal casts. Analysis of these sedimentary facies suggests that in southeastern Colorado, lakes were larger and longer lived than in other Morrison paleoenvironments.
Trackway orientations and footprint-depth contours pinpoint the location of the paleoshoreline at successive levels in the section. Detailed mapping of 1,300 footprints in bed 2 has revealed more than 100 trackways which testify to the activity of both quadrupedal and bipedal dinosaurs. The respective ratio based on trackway counts is ∼40/60. The quadrupedal tracks are attributed to sauropods, and represent the first ever discovered in North America. They also exhibit the first known manus claw impressions and are in need of formal description. More than 90% of the bipedal tridactyl prints lack claw impressions and are tentatively therefore referred to the Ornithopoda, cf. Gypsichnites possibly a Camptosaurus . The small proportion with distinct claw impressions may represent Allosaurus . Resulting estimates of species diversity (∼5) and predator-prey ratio (1:30) are in general agreement with the estimates of other authors based on skeletal remains, and they suggest that the Purgatoire River tracks may accurately reflect the composition of the dinosaur fauna. Distinctive groupings of parallel, non-overlapping trackways suggest gregarious behavior among sauropods and tridactylous forms.
TL;DR: The fact that seasonal patterns were seen only in users, and not in nonusers, of electrically heated beds suggests that electrical bed heating may have a direct effect on fetal development.
Abstract: Seasonal patterns were seen in fetal growth and in abortion rate for families using electrically heated beds. These patterns could be attributed to the seasonal use of heated beds. The fact that such seasonal patterns were seen only in users, and not in nonusers, of electrically heated beds suggests that electrical bed heating may have a direct effect on fetal development. The effect could be due to excessive heat or to electromagnetic field exposure.
TL;DR: It is reported that no HTLV-I-like or LAV/HTLV-III-like sequences can be detected, by in situ hybridization, in central nervous system (CNS) tissues from MS patients, and that no specific HTLV -I- like signal in peripheral blood mononuclear cells or in CSF cell lines is characteristic of MS.
Abstract: Several observations suggest that retroviral infection is involved in the pathogenesis of the human demyelinating disease multiple sclerosis (MS). First, lymphadenopathy-associated virus/human T-lymphotropic virus type III (LAV/HTLV-III), the agent of acquired immune deficiency syndrome (AIDS), has been shown to be neurotropic in man. Second, the genetic organization of the lentivirus visna, which causes a chronic demyelinating disease of sheep, closely resembles that of LAV/HTLV-III. Recently, Koprowski and colleagues reported that MS is associated both with raised levels of circulating antibodies to HTLV-I and with the presence of HTLV-I-specific RNA within cell lines derived from the cerebrospinal fluid (CSF). Here we report that no HTLV-I-like or LAV/HTLV-III-like sequences can be detected, by in situ hybridization, in central nervous system (CNS) tissues from MS patients, and that nonspecific HTLV-I-like signal in peripheral blood mononuclear cells or in CSF cell lines is characteristic of MS. Furthermore, enzyme-linked immunosorbent assay (ELISA) analysis of circulating and CSF antibodies for HTLV-I reactivity fails to distinguish between MS and control groups.
TL;DR: This article used meta-analytic techniques to examine the race effect size for objective measures of performance and to compare the relationship between effect sizes for objective indices and subjective ratings and found that subjective ratings had a lower effect size than objective cognitive test scores but were higher than comparable objective performance indices.
Abstract: Although the criterion problem has been acknowledged as critical in personnel research, few attempts have been made to systematically examine the nature and covariates of criterion measures of performance. The present research used meta-analytic techniques to examine the race effect size for objective measures of performance and to compare the relationship between effect sizes for objective indices and subjective ratings. Fifty-three studies were located that included at least one objective index of actual performance, absenteeism or cognitive test performance and one subjective measure of performance for the same group of black and white employees. The corrected average effect sizes across the 53 studies were relatively low but quite similar for the objective and subjective criteria. Moderating effects for the objective criteria were found as race effects were much higher for cognitive than for performance criteria. Subjective ratings had a lower effect size than objective cognitive test scores but were higher than comparable objective performance indices. The implications of the results for personnel research practices were discussed and the need for a better understanding of the constructs underlying criterion measures was emphasized. A Study of Race Effects
TL;DR: Chromosome aberration frequencies in 61 employees potentially exposed to ethylene oxide (ETO) were compared with those in unexposed control groups and significant increases in aberrations associated with smoking and with increasing age were found.
Abstract: Chromosome aberration frequencies in 61 employees potentially exposed to ethylene oxide (ETO) were compared with those in unexposed control groups. We studied 3 worksites with differing historical ambient levels of ETO. Within worksites, groups were classified as high potential exposed, low potential exposed, or controls. Further control groups including an off-site community control group were added to give a total of 304 control individuals. Blood samples were drawn several times over a 24-month period. Aberrations were analyzed in 100 cells per sample after culture for 48–51 h. Worksites I, II and III respectively represented increasing levels of potential ETO exposure. At worksites I and II, no consistent differences in aberration frequencies were found among groups. At worksite III aberration frequencies in potentially exposed individuals were significantly increased compared with controls. The frequencies of cells with aberrations were 5.6% for the 2 individuals in the high potential exposure category and 2.6% for 23 persons in the low potential exposure group. The overall frequency of cells with aberrations in the matched control individuals was 1.4%. In the total control group of 304 individuals we found significant increases in aberrations associated with smoking and with increasing age. We have also reported previously an association between sister-chromatid exchange (SCE) frequency and ETO exposure (Stolley et al., 1984). When aberration frequencies were compared with levels of SCEs there was only a weak overall association. The correlation was found in potentially exposed but not in control groups, and for any individual, one observation could not be used to predict the other.
TL;DR: Five of seven acutely manic patients improved significantly when taking verapamil but not placebo in a double-blind crossover study, adding support to two other formal studies of verAPamil and several case reports of verabamil in manic patients.
Abstract: Five of seven acutely manic patients improved significantly when taking verapamil but not placebo in a double-blind crossover study. This finding adds support to two other formal studies of verapamil, several case reports of verapamil in manic patients, and one small study of nifedipine suggesting that some calcium antagonists may have antimanic properties. Indirect evidence links this antimanic action to correction of a disturbance of intracellular calcium dynamics in affective disorders. However, the effectiveness of calcium antagonist drugs could also be related to some property other than interference with the action of calcium within brain neurons.
TL;DR: Data suggest that the pattern of infections manifested in AIDS could provide clues about transmission and that there may be a hierarchy of reactivation of latent infections in which populations with exposure to multiple agents manifest these preferentially to Pneumocystis carinii.
Abstract: The frequency of nine reactivating or opportunistic infections and Kaposi's sarcoma among patients with the acquired immunodeficiency syndrome (AIDS) was reviewed. The diagnoses of 87 patients reported from the Colorado AIDS registry and 359 others from literature reports were abstracted, and data were placed in one of 11 categories on the basis of the risk group of the patient. Pneumocystis carinii infection was significantly commoner among blood or blood-product recipients than among natives of the tropics (P < .001). Tuberculosis and toxoplasmosis each were significantly commoner among natives of the tropics than natives of developed countries (P < .001), whereas disseminated Mycobacterium avium-Mycobacterium intracellulare infections were present more often in the latter group. Among natives of the tropics treated in developed countries, cytomegaloviral infection was diagnosed significantly less often (22%) than among persons from developed countries in whom sexual transmission was presumed (47%; P = .0005). These data suggest that the pattern of infections manifested in AIDS could provide clues about transmission and that there may be a hierarchy of reactivation of latent infections in which populations with exposure to multiple agents manifest these preferentially to Pneumocystis carinii.
TL;DR: The coding region for the small-subunit rRNA gene from Paramecium tetraurelia is sequenced, indicating that the Ciliophora diverged from the eukaryotic line of descent as a loose phylogenetic grouping during a radiative period that gave rise to the Fungi, the Plantae and the Metazoa.
Abstract: We have sequenced the coding region for the small-subunit rRNA gene fromParamecium tetraurelia. Similarity comparisons between smallsubunit rRNAs from representative of the Metazoa, the Plantae, the Fungi and four other members of the Ciliophora were used to construct phylogenetic trees. In these phylogenies the Ciliophora diverged from the eukaryotic line of descent as a loose phylogenetic grouping during a radiative period that gave rise to the Fungi, the Plantae and the Metazoa.
TL;DR: Two groups of patients with congestive heart failure were studied, one with elevated (Group I) and another (Group II) with suppressed plasma concentrations of vasopressin, and improved cardiac performance was associated with enhanced water excretion.
TL;DR: This study was undertaken to determine the relationship of the IAW, measured between at the widest dimension of the alae of the nose, and two other measurements of maxillary anterior teeth, which are significant in the selection and arrangement of artificial teeth used in complete dentures.
Abstract: This study was undertaken to determine the relationship of the IAW, measured between at the widest dimension of the alae of the nose, and two other measurements of maxillary anterior teeth. The other measurements, both of which are significant in the selection and arrangement of artificial teeth used in complete dentures, were the ICTW and the ARCD measured from the distal surface of the maxillary canine on one side of the arch to the distal surface of the canine on the opposite side of the arch. No distinction was made between either the sex or the age of the subjects. The mean ICTW of 35.35 mm was 3% greater than the mean IAW of 34.28 mm. The mean ARCD of 44.85 mm was 31% greater than the mean IAW of 34.28 mm. This mean ARCD compares favorably (less than 1 mm difference) with a constructed ARCD of 45.8 mm from a tooth dimension study by Shillingburg et al. The ARCD was 44.85 mm. This measurement had not been made in the previous study examining the relationship of interalar distance to ICTW. When the IAW was plotted against the intercanine cusp tip width, a fairly strong correlation coefficient of 0.413 was exhibited. A weaker but definite correlation coefficient of 0.217 was observed when the interalar width plotted against the circumferential arc distance from distal surface to distal surface of the maxillary canines.
TL;DR: In this article, identical twins who have a developmental form of the syndrome were described, aged 41 years, were the product of a normal pregnancy and birth, but had subsequent delayed motor milestones, seizures, poor language development, mild mental retardation, drooling, inability to protrude the tongue, brisk jaw jerks, impaired fine finger movements, symmetrical brisk reflexes, flexor plantar responses, and mildly spastic gait.
Abstract: Foix, Chavany, and Marie described a syndrome of faciopharyngoglossomasticatory diplegia resulting from bilateral anterior opercular infarction. We describe identical twins who have a developmental form of the syndrome. The twins, aged 41 years, were the product of a normal pregnancy and birth, but had subsequent delayed motor milestones, seizures, poor language development, mild mental retardation, drooling, absent gag reflexes, inability to protrude the tongue, brisk jaw jerks, impaired fine finger movements, symmetrical brisk reflexes, flexor plantar responses, and mildly spastic gait. Magnetic resonance imaging showed bilateral perisylvian cortical dysplasia compatible with polymicrogyria and incomplete opercular formation.
TL;DR: Diffuse neonatal hemangiomatosis is an often fatal disorder characterized by widespread capillary hemangiomas of the skin and visceral organs, and the organs most commonly affected are the gastrointestinal tract, brain, liver, and lung.
Abstract: Diffuse neonatal hemangiomatosis is an often fatal disorder characterized by widespread capillary hemangiomas of the skin and visceral organs. Ultrasound and computed tomographic scans may be useful in determining the extent of visceral disease. The organs most commonly affected are the gastrointestinal tract, brain, liver, and lung. Complications include high-output cardiac failure, gastrointestinal bleeding, hydrocephalus, and consumption coagulopathy. Despite therapy with corticosteroids, the mortality rate is high.
TL;DR: Characterization of this membrane-associated protein indicated that it was soluble in solutions containing Triton X-100, was capable of binding folic acid as well as methotrexate, had an apparent Mr of 160,000 by gel filtration in the presence of Trit on X- 100, and was precipitated by antiserum to human placental folate receptor.
TL;DR: Results suggest that disinfection procedures commonly used for treatment of drinking water to remove coliform bacteria are adequate to eliminate C. jejuni and further correlate with the absence of outbreaks associated with properly treated water.
Abstract: Campylobacter jejuni and closely related organisms are important bacterial causes of acute diarrheal illness in the United States. Both endemic and epidemic infections have been associated with consuming untreated or improperly treated surface water. We compared susceptibility of three C. jejuni strains and Escherichia coli ATCC 11229 with standard procedures used to disinfect water. Inactivation of bacterial preparations with 0.1 mg of chlorine and 1.0 mg of monochloramine per liter was determined at pH 6 and 8 and at 4 and 25 degrees C. Under virtually every condition tested, each of the three C. jejuni strains was more susceptible than the E. coli control strain, with greater than 99% inactivation after 15 min of contact with 1.0 mg of monochloramine per liter or 5 min of contact with 0.1 mg of free chlorine per liter. Results of experiments in which an antibiotic-containing medium was used suggest that a high proportion of the remaining cells were injured. An animal-passaged C. jejuni strain was as susceptible to chlorine disinfection as were laboratory-passaged strains. These results suggest that disinfection procedures commonly used for treatment of drinking water to remove coliform bacteria are adequate to eliminate C. jejuni and further correlate with the absence of outbreaks associated with properly treated water.
Journal Article•
TL;DR: These experiments provide evidence for a functional coupling among GABA receptors and the chloride ionophore and suggest that the GABA-activated chloride channel is a site of action for several convulsant compounds.
Abstract: gamma-Aminobutyric acid (GABA), the major inhibitory neurotransmitter in the mammalian brain, increases membrane chloride conductance. Previously, we reported that GABA increases 36Cl- uptake by membrane vesicles (microsacs) prepared from mouse brain. Employing this technique, we found that the GABAA agonists, muscimol, isoguvacine, 4,5,6,7-tetrahydroisoxazolo(5,4-C)pyridine-3-ol, and 3-amino-1-propane sulfonate, all produced a concentration-dependent increase in 36Cl- influx, but baclofen, a GABAB agonist, failed to alter 36Cl- flux. Inhibition of GABA-dependent 36Cl- influx was produced by the convulsant drugs, bicuculline, picrotoxin, and pentylenetetrazole. Ion specificity was demonstrated by a failure of GABA agonists to stimulate influx of 45Ca2+, 86Rb+, 22Na+, or 35SO4(2). GABA-stimulated uptake of 36Cl- was largest in cortex and cerebellum and smaller in hippocampus and striatum. There was little difference in sensitivity to GABA among the areas. Analysis of subcellular fractions prepared from mouse brain demonstrated that the GABA-dependent 36Cl- influx was enriched in the synaptosomal fraction. The nonspecific (GABA-independent) uptake of 36Cl- was enriched in the myelin fraction. These experiments provide evidence for a functional coupling among GABA receptors and the chloride ionophore and suggest that the GABA-activated chloride channel is a site of action for several convulsant compounds.
TL;DR: A surgical technique was developed to allow injection of B16 melanoma cells into the embryo in utero, which correlated with the time of arrival of normally migrating premelanocytes into the skin.
Abstract: This report focuses on the regulation of murine melanoma by the embryonic skin. A surgical technique was developed to allow injection of B16 melanoma cells into the embryo in utero. A significant decrease in incidence of tumors was noted, which correlated with the time of arrival of normally migrating premelanocytes into the skin. Media were conditioned from skin explanted at the time premelanocytes arrive in it; these media inhibited the growth of melanoma cells in vitro. Under optimal conditions the growth of melanoma cells ceased; the cells had altered morphology and failed to proliferate when placed in fresh growth media.
TL;DR: Phenothiazines and tricyclic antidepressants, when added to culture medium, gave rise in several types of cells, to a decrease in lysosomal sphingomyelinase activity, and the metabolic pathway implying the synthesis of phosphatidylcholine from the hydrolysis of sphedomyelin and/or transfer of phosphorylcholine to phosphatilcholine was partially reduced.
TL;DR: Evidence is presented that there is specific binding of anti-Ro (SS-A) antibodies to Ro (SS -A) antigen in the skin, and it is proposed that these antibodies may be directly involved in cutaneous disease.
Abstract: To study antibody binding in cutaneous lupus, we used human skin grafted onto nude mice. By immunofluorescence examination, mice injected with anti-Ro (SS-A) sera from subacute cutaneous or neonatal lupus erythematosus patients showed evidence of human IgG deposited in the skin, while mice injected with anti-native DNA or normal sera did not. We present evidence that there is specific binding of anti-Ro (SS-A) antibodies to Ro (SS-A) antigen in the skin, and we propose that these antibodies may be directly involved in cutaneous disease.
TL;DR: Comparing icosanoid measurements obtained either with EIA or RIA with those obtained by gas chromatography/mass spectrometry (GC/MS) found that they can give accurate and reproductive results in lung tissue, although LTC 4 and 5-HETE must be purified prior to analysis.