Showing papers in "Analytical Biochemistry in 2001"

TL;DR: This forward-looking article describes a new opportunity in fluorescence, radiative decay engineering (RDE), and predicts that nearby metal surfaces can be used to increase the low intrinsic quantum yields of nucleic acids and make unlabeled DNA detectable using its intrinsic metal-enhanced fluorescence.

TL;DR: Aptamer beacon can be a sensitive tool for detecting proteins and other chemical compounds and a fluorescence-quenching pair is used to report changes in conformation induced by ligand binding.

TL;DR: The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has recently leapt to prominence within numerous biological fields and has become a truly versatile marker for visualizing physiological processes, monitoring subcel-based detection, and more.

TL;DR: D duplex relative RT-PCR procedure including ribosomal 18S RNA as internal standard and competimer technology is precise for RNA quantification and is tailored for cDNA array validation, providing molecular evidence that cellular subpopulations of the same pathological origin are highly heterogeneous.

TL;DR: An algorithm was developed which identifies sensitivity factors, thereby allowing the rapid quantitation and molecular species fingerprinting of TAG molecular species directly from chloroform extracts of biological samples.

TL;DR: In this study a ninhydrin method for measuring the total protein content of tissue hydrolysates is presented, which was several times more sensitive than the Coomassie reaction and linear over a greater range of protein concentration.

TL;DR: Preliminary assays that use up-converting phosphor labels, including tests for a drugs of abuse panel and Escherichia coli O157:H7, have been developed and demonstrate performance comparable with lab-based, commercially available EIAs.

TL;DR: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry is an established tool for the analysis of proteins, whereas it gained by far less interest in the field of lipid analysis, but this method works well with phospholipids as well as organic cell extracts and provides high sensitivity and reproducibility.

TL;DR: This nondestructive method is completely general, enabling precise analysis of reactions in spectroscopically opaque solutions, using physiological substrates, and may have wide applicability in functional genomics.

TL;DR: The use of infrared-mediated temperature control to accurately thermocycle microliter volumes in microchips fabricated from polyimide is demonstrated and adequate amounts of PCR product are observed after only 15 cycles.

TL;DR: UCP lateral flow assays can be used for applications that are currently limited by assay sensitivity, and they can increase the probability of a diagnosis by verifying the presence of several analytes in the same sample.

TL;DR: Real-time quantification and mutation detection with a simple single labeled probe is demonstrated by using the inherent quenching of deoxyguanosine nucleotides in the amplicon, complicated probe designs involving internal quenched can be avoided.

TL;DR: These studies demonstrate that CTD110.6 is highly specific toward O-GlcNAc, with no cross-reactivity toward similar carbohydrate antigens or toward peptide determinants.

TL;DR: The results indicate that the expression level of a constitutively expressed gene may change during the cell culture in vitro, which emphasizes again the importance of carefully validating endogenous control genes for comparative quantification.

TL;DR: Using warfarin as a test system, the application of BIACORE SPR biosensors are validated to reliably determine binding constants for drug/HSA interactions and it is shown that the % bound values determined by SPR correlate with thevalues determined by solution-based methods.

TL;DR: A stable-isotope dilution, liquid chromatography-mass spectrometry assay for the direct quantitative analysis of 1-acyl-LPA, using a deuterium-labeled internal standard, LPA, and a single liquid-liquid extraction with acidic butanol that allows >95% recovery of LPA.

TL;DR: A novel technique for high-throughput simultaneous screening of multiple cytokine expression based on a protein array system that has the advantage of showing the specificity of enzyme-linked immunosorbent assays, sensitivity of enhanced chemiluminescence (ECL), and high-Throughput of microspot.

TL;DR: The following lysophospholipid species are significantly increased in ascites from patients with ovarian cancer, compared to patients with nonmalignant diseases (e.g., liver failure): LPA (including acyl-, alkyl-, and alkenyl-LPA species), lysphosphatidylinositol, and sphingosylphosphorylcholine.

TL;DR: Quantification of intracellular concentrations of glycolytic intermediates and nucleotides in Escherichia coli K12 using a perchloric acid extraction and an LC-ESI-MS method was achieved and it is thus possible to identify and quantify more than 15 intrACEllular metabolites in parallel with a minimal amount of sample volume.

TL;DR: It is revealed that the amide I, II, and III bands differ in their sensitivities to changes in protein conformation: For example, strong bands in the region 1620-1630 and 1685-1695 cm(-1) were seen in the amid I region of aggregated protein spectra, while absorbance of such magnitudes was not observed in theAmide II and III region.

TL;DR: The newly developed HPAEC method could provide valuable information necessary for generating sialylated complex-type N-glycans in insect or other cells, either native or genetically manipulated.

TL;DR: The systematic evaluation of important parameters of the labeling protocol is described and optimized labeling conditions using the optimized conditions specific and quantitative labeling was achieved on standard proteins as well as in complex protein mixtures such as a yeast cell lysate.

TL;DR: This is the first time that a hormonal cycle has been described for individuals based on NMR spectroscopic and multivariate analysis of metabolic data and shows the value of metabonomic methods in the investigation of physiological variation and rhythms.

TL;DR: TRAP-ELIDA is a simple and sensitive method to quantify telomerase activity without time-consuming gel electrophoresis and could be applied to a large number of clinical samples at the same time.

TL;DR: Based on molecular weights and the molar ratio, an immune complex, consisting of alternating three D2E7 and three TNFalpha molecules, is proposed as the most stable complex.

TL;DR: A new colorimetric detection method is presented which is intented to make the use of microarray a powerful procedure and a low-cost tool in research and clinical settings.

TL;DR: A real-time PCR assay to detect the presence of Escherichia coli O157:H7 using molecular beacons, designed to recognize a 26-bp region of the rfbE gene, coding for an enzyme necessary for O-antigen biosynthesis, was described.

TL;DR: The Langmuir-Freundlich model appeared to be the most efficient model for explaining the interactions of proteins with IMA-M(II) gels, and was able to explain cooperativity and binding heterogeneity in quantitative terms.

TL;DR: An analytical method which enables us to perform hydroxytyrosol and tyrosol quantitative determinations in human urine is described, which was successfully used in bioavailability studies of both phenolic compounds after acute olive oil administration.

TL;DR: Pseudomonas aeruginosa secretes multiple proteases that have been implicated as virulence factors and the detection of each specific enzyme can be difficult to determine, so three specific assays for protease IV are identified, a new assay specific for alkaline protease is identified, and it is shown that proteaseIV has a distinct enzymatic specificity relative to the three other Pseudomona proteases.