Showing papers in "Bioconjugate Chemistry in 1993"

TL;DR: It is shown, using luciferase and beta-galactosidase containing plasmids, that dendrimers mediate high efficiency transfection of a variety of suspension and adherent cultured mammalian cells.

TL;DR: A series of new fluorescent labeling reagents based on sulfoindocyanine dyes contain succinimidyl ester reactive groups and can be readily conjugated to antibodies, avidin, DNA, lipids, polymers, and other amino-group-containing materials.

TL;DR: DTT reduction of disulfides present in the mAb was a reliable and general method for generating a consistent number of reactive SH groups and BR96 conjugates showed antigen-specific cytotoxicity.

TL;DR: These OIPAAm-biomolecule conjugates maintain their temperature responses, are soluble in cold water, and precipitate over a range of temperatures related to oligomer content, indicating the potential of strategy in reversible bioreactors and protein separations.

TL;DR: Synthesis of a distearoylphosphatidylethanolamine-polyethylene glycol-PEG conjugate, bearing a hydrazide group at the unattached end of the polymer chain, was achieved using a new heterobifunctional polymeric reagent.

TL;DR: The preparation and in vitro characterization of tumor-specific superparamagnetic particles (SMP) are described and the polypeptide coat chosen provides an ideal platform for the attachment of biological modifiers needed for the reduction of the antigenicity and blood clearance rate of anti-CEA SMP.

TL;DR: The major modified PEG--papain variant was demonstrated to be 5000 Da larger than the unreacted papain, and characterized by matrix-assisted laser desorption mass spectrometry, SDS-PAGE, and high performance gel filtration chromatography.

TL;DR: Preliminary studies show that the conjugated enzyme exhibits very high retention of activity, even higher than native enzyme, and shows improved thermal stability compared to native enzyme.

TL;DR: Temperature-responsive OIPAAm-collagen conjugates are expected to maintain native collagen functionality in the solution state, react at lower temperatures, and be easily removed from the system with small temperature increases.

TL;DR: Results indicate that poly(PEG-Lys), a new, water-soluble poly(ether urethane), derived from L-lysine and poly(ethylene glycol), is a promising precursor for the preparation of soluble drug conjugates.

TL;DR: The results demonstrate that biologically active antibody/cytokine fusion proteins can be constructed by genetic engineering and may require constant infusion rather than bolus injection in order to achieve clinical efficacy.

TL;DR: It was determined that two fluorine substituents, ortho and ortho' substituted relative to the azide group, are required to retard ring expansion and allow bimolecular capture of the singlet nitrene.

TL;DR: An improved method for the activation of polyethylene glycol with commercially available succinimidyl carbonate with high yield was described and was coupled to proteins in high yield.

TL;DR: Light-dependent oriented and covalent immobilization of target molecules has been achieved by combining two modification procedures: light-dependent coupling of target molecule to inert surfaces and thiol-selective reactions occurring at macromolecule or substrate surfaces.

TL;DR: This study presents the specific hybridization of complementary DNA fragments as a novel recognition mechanism in pretargeting and demonstrates that the radiolabeled DNA is still capable of hybridizing selectively with the oligonucleotides of the immunoconjugate, when the latter is bound to its antigen.

TL;DR: Three bisacridine intercalators containing a galactose residue(s) to target DNA to cell surface receptors for use in gene-delivery systems and binding of the (Gal-6)3Lys2-bA-DNA to the hepatocytes could be blocked by asialoorosomucoid.

TL;DR: A novel DOTA derivative is synthesized by directly coupling a tetrapeptide to DOTA through an amide bond, and it is shown that the radiolabeled conjugates are kinetically inert under physiological conditions.

TL;DR: A totally new pathway for the introduction of rhenium in the form of low oxidation state organometallic complexes covalently attached to various proteins is described and response was found to be related to the coupling extent of the conjugate.

TL;DR: To study the importance of the disulfide bonds of the toxin to the activity of single-chain immunotoxins, mutants in which either the cysteines in the toxin were changed to alanines or the amino acids 365-380 of PE were deleted were constructed.

TL;DR: IL6-PE4E purified in this manner was fully cytotoxic toward human multiple myeloma, hepatoma, epidermoid carcinoma, and prostate carcinoma cell lines and found the dose-limiting toxicity to be to the liver, by measurement of serum transaminases and histologic evaluation of the liver.

TL;DR: Full enzymatic activity was regained after the immediate addition of hydroxylamine to the inactivated chymotrypsin and PPE derivatives, which indicated that a simple acyl enzyme derivative is formed initially in the inhibition reaction.

TL;DR: PMPI is a heterobifunctional cross-linking agent useful for thiol to hydroxyl coupling and activation of vitamin B12, digoxigenin, digitoxigen in, estradiol, progesterone, and some serine-containing peptides.

TL;DR: Under optimal conditions the efficiency of cell transformation with IPECs is several fold higher than that observed during standard calcium phosphate precipitation.

TL;DR: The X-ray diffraction pattern of PEG1-SF indicated that the PEG molecules covalently bonding to SF narrowed the spacing of the interchain periodicity and promoted the formation of theinterchain beta-sheet.

TL;DR: Several fluorinated aryl azides were studied by laser flash photolysis techniques to determination of the rate constant for ring expansion of the singlet nitrene to the dehydroazepine and measurement of the absolute rate constants for reaction of the singinglet nitrenes with typical quenchers.

TL;DR: A cluster of five drug molecules may thus be attached to a single site on a protein, giving a relatively homogeneous product in spite of the high drug conjugation ratio.

TL;DR: The results obtained indicate that substrate-size-dependent specificity of PEG-modified enzymes cannot be explained solely by steric hindrance considerations and the decreased activity of P EG-enzymes toward protein substrates is consistent with the well-documented ability of PPD to exclude proteins from its surroundings.

TL;DR: The covalent attachment of MTX to branched polypeptides results in a reduction of drug in vitro cytotoxicity influenced by the carrier structure, andjugation to amphoteric polymers, depending on the configuration and position of glutamic acid resulted in a decrease of anti-791T cell activity.

TL;DR: In vivo experiments in nude mice demonstrated that CCM was less toxic than CM, and that both prodrugs were much less Toxic than PDM, and neither CCM nor PDM exerted antitumor activity on subcutaneous H2981 tumors in vivo.