Showing papers in "Enzyme and Microbial Technology in 2017"

TL;DR: Results suggested that CALB molecules immobilized on octyl agarose may be closely packed together due to the high immobilization rate and this produced some negative interactions between immobilized enzyme molecules during enzyme thermal inactivation, so slowing-down the immobilization rates may be a solution for this unexpected problem.

TL;DR: Results indicate that TCM might be used as a complementary and alternative approach to the primary and secondary prevention of cardiovascular disease, however, further rigorously designed randomized controlled trials are warranted to assess the efficacy and safety of TCM on longterm hard endpoints in patients with cardiovascular disease.

TL;DR: The results show that the detergent properties of some reaction products increase the enzyme release from the hydrophobic support, and this problem increased if the concentration of the reactants increased.

TL;DR: The biosynthesis of Ag NPs is reported, for the first time, using identified antimicrobial molecules ( gallic acid+apocynin) and (gallic Acid+apOCynin+quercetin) from the medicinal plant Pelargonium endlicherianum Fenzl.

TL;DR: The fabricated immunosensor demonstrated excellent sensitivity, stability and reproducibility and therefore was successfully applied to analyse PSA in biological samples and indicates that the proposed immunos sensor has potential in clinical screening of cancer biomarkers.

TL;DR: This study provides a comprehensive insight into the community structure and diversity of the SNPR system, and will provide foundation for optimal operation of nutrient removal systems.

TL;DR: The findings strongly indicated that the AgCl-NPs from C. vulgaris conditioned medium is a promising 'green' alternative for biomedical application as antimicrobials.

TL;DR: This result suggests that the intermediate hydrogen peroxide is not decomposed by catalase due to a short diffusion distance between GOx and HRP in the combi-CLEA, which contains two enzymes glucose oxidase and horseradish peroxidase which are cross-linked together as solid aggregates.

TL;DR: It is demonstrated that the presence of CuO bonds in NSBs could be indication of VO extract-Cu2+ complexes, and the NSBs exhibited peroxidase-like activity towards guaiacol used as a model substrate depending on Fenton-like reaction.

TL;DR: In this article, a color screening system was developed and adopted for directed evolution of β-carotene ketolase (OBKT), generating a triple mutant OBKTM (H165R/V264D/F298Y) with 2.4-fold improved activity.

TL;DR: A simple but efficient method to synthesize highly robust enzyme-inorganic hybrid nanoflowers, which relies on further crosslinking of the enzyme molecules entrapped in the hybrid nan oflowers by treatment with glutaraldehyde by employing lipase from Candida rugosa as a model enzyme.

TL;DR: In general, PEI treatment was the most effective in increasing enzyme stability, while glutaraldehyde had a milder stabilization effect, and crowded enzyme preparations were intermolecular crosslinked using Glu plus PEI, but some molecules still remained non-crosslinked.

TL;DR: It is suggested that while pH control in the cathode chamber is important, it does not guarantee sustained laccase activity; as salinity increases affect the activity and it could be mitigated using a cation selective membrane.

TL;DR: An improved amperometric l-lactate biosensor was constructed based on covalent immobilization of lactate oxidase (LOx) from Pediococcus species onto carboxylated multiwalled carbon nanotubes/copper nanoparticles/polyaniline hybrid film electrodeposited on the surface of a pencil graphite electrode (PGE).

TL;DR: In this work, physical adsorption was used for immobilization of proline dehydrogenase onto a magnetic mesoporous silica nanomaterial, which led to better resistance to temperature and pH inactivation in comparison to the free enzyme.

TL;DR: This review exposed knowledge gaps that should be covered in future work in order to fully understand how enzyme synergism works and the effect of lignocellulose degradation products of one enzyme on the behaviour of the other enzyme and ultimately their synergistic relationship.

TL;DR: The phylogenetic analysis showed that LipJ2 clusters apart from other members of known lipase families, indicating that the newly isolated Janibacter esterase LipJ 2 would be the first characterized member of a new family of bacterial lipases.

TL;DR: Factors that affect the performance of MECs are discussed and critically analysed, and the potential for scale-up of H2 bioelectrosynthesis is also discussed.

TL;DR: The first report on association of lignin peroxidase in the synthesis of AuNP and SeNP from Acinetobacter sp.

TL;DR: The enzymatic characteristics research showed that ToGI had higher catalytic efficiency and superior thermostability toward d-glucose among the screened GIs, which might be promising for realization of future industrial production of HFCS at elevated temperature.

TL;DR: This laccase, CueO from Escherichia coli K12 in Pichia pastoris GS115, was applied on the decolorization of wastewater from a textile printing factory and showed an obvious bleaching effect.

TL;DR: Thermal stability, resistance to pH, recycling and storage capacity were improved as compare to free lipase, and the two kinds of yolk/shells composites were obtained.

TL;DR: A mind-body program for chronic LBP improved short-term function and long-term current and most severe pain and the functional improvement was not sustained, suggesting that future development of the intervention could focus on durability.

TL;DR: This paper describes the recombinant expression and characterization of a new and highly active cold-adapted xylanase from the GH-family 10 (Xyl-L), and the use of a novel one step combined directed evolution technique that comprises saturation mutagenesis and focused epPCR as a feasible semi-rational strategy to improve the thermostability.

TL;DR: It was observed that the investigated support materials influenced the immobilization of hydrogen producing bacteria and consequently hydrogen production performance as well as composition of soluble metabolites.

TL;DR: The use of biosynthesized AgNPs as effective growth inhibitors for various biomedical applications is suggested.

TL;DR: The final strain (WTXB) was able to reach up to 98% of the theoretical yield (25% higher as compared to the first study), the highest reported value for EG production from d-xylose.

TL;DR: An improved amperometric triglyceride (TG) biosensor was fabricated and employed for determination of TG in the serum of apparently healthy subject and persons suffering from hypertriglyceridemia, and lost 20% of its initial activity after its continued uses over a period of 240days.

TL;DR: It was showed that butanol addition had multiple effects on Schizochytrium limacinum B4D1, and when cells were treated with 8g/L butanol, the astaxanthin content increased to 245 times than that of the untreated control.

TL;DR: Once hydrolyzed into fermentable sugars, soybean carbohydrate can find more value-added applications and further improve the overall economics of soybean processing.