Showing papers in "Fungal Genetics and Biology in 2012"

TL;DR: An approach was initiated to establish an expression system for B. cinerea possessing the following features: the targeted integration of constructs at defined gene loci which are dispensable under standard growth conditions, the use of promoter and terminator sequences allowing optimal gene expression, and the incorporation of a highly efficient cloning system.

TL;DR: One of the functions of BglR is to up-regulate specific β-glucosidase genes (with the exception of bgl1, which is seemingly under the direct control of Xyr1).

TL;DR: Microarray analysis of RNA derived from culture conditions that induce differential gene expression can be an effective tool for identifying SM biosynthetic gene clusters in F. verticillioides.

TL;DR: Putative homologs of the bacterial IaaM and IaaH genes in four Fusarium species are identified and the regulation of the IAM genes and the limiting factors of IAA production via the I AM pathway are discussed.

TL;DR: Of the 30 species in China, 20 appear to be endemic, nine were also represented in Europe, and four putatively fire-adapted species have disjunct distributions inChina, Europe and western North America.

TL;DR: Gen deletion analysis was employed to assess the effect of loss of a F. verticillioides laeA orthologue, LAE1, on genome-wide gene expression and secondary metabolite production and revealed that, in contrast to a previously described Fusarium fujikuroi lae1 mutant, bikaverin production is reduced.

TL;DR: An initial clonal distribution through asexually produced conidiospores as observed for other fungal pathogens holds not true for H. pseudoalbidus, and the data is highly supportive for a distribution through ascospores.

TL;DR: Phylogenetic analyses of a 71-protein dataset support the position of Wallemia as the earliest diverging lineage of Agaricomycotina, which is confirmed by septal pore ultrastructure that shows the septAL pore apparatus as a variant of the Tremella-type.

TL;DR: Using live-cell imaging, non-lytic expulsion/exocytosis of C. albicans from macrophages is demonstrated for the first time and following complete expulsion, both the phagocyte and pathogen remain intact and viable.

TL;DR: The data indicate that endogenous EGT help protect conidia during the quiescent period between conidiogenesis and germination, and that EGT helps protect conidium during the germination process from the toxicity of peroxide but not from superoxide or Cu(2+).

TL;DR: Results indicate that Bbslt2 plays an important role in conidiation, viability, cell wall integrity and virulence in B. bassiana.

TL;DR: The majority of species lost their ability to sexual reproduction and the K/θ method is a useful measure to delineate species in the Longibrachiatum clade.

TL;DR: Results from the present work suggest a linkage between VGB and VdPKAC1 signaling pathways in regulating virulence, hormone production and development in V. dahliae.

TL;DR: ManR is a positive regulator of the β-mannan utilization system in Aspergillus oryzae, the first study to identify a transcriptional regulator of this system in eukaryotic cells.

TL;DR: It is demonstrated that NO could effectively alleviate oxidative damage induced by heat stress in mycelia of Pleurotus eryngii var.

TL;DR: Results of this study indicated that FgVelB plays a critical role in the regulation of various cellular processes in F. graminearum.

TL;DR: Data suggest a role in cell wall remodeling during hyphal growth for chit-1, and connection between gh18-10 ENGase activity and the endoplasmic reticulum associated protein degradation process, a stringent quality control of glycoprotein maturation, is discussed.

TL;DR: An endophyte such as F. verticillioides may function as both a defensive mutualist and a parasite, and express nutritional modes that depend on ecological context, as well as co-inoculation of the endophytes with the pathogen.

TL;DR: Simultaneous examination of morphological development and stage-specific gene expression suggests that degeneration of the paraphyses during sexual development is an apoptotic process, and differences in gene expression reflect the greater role of fruiting bodies in the life cycle and ecology of F. verticillioides.

TL;DR: DNA repeats associated with the uORFs suggest that 3' gene duplication may be an adaptive mechanism to modify coding regions or generate new ORFs, and contrasts to the wide-scale size reduction experienced by fungal mt genomes.

TL;DR: Heterochromatin protein characterized in F. graminearum regulates secondary metabolism in this fungus and could be conserved mechanism in ascomycetes.

TL;DR: The results indicate that G. marginata biosynthesizes amatoxins on ribosomes by a pathway similar to Amanita species, involving a genetically encoded proprotein of 35 amino acids that is post-translationally processed by a POP.

TL;DR: In liquid culture further pellet formation is unaffected by altered spore-spore interactions, indicating that germ tube and hyphal adherence can compensate for deficiencies in the initial step of spore attachment.

TL;DR: Phylogenetic analysis of PKS genes suggests that all main types of fungal iterative PKS had already evolved before the Ascomycota and Basidiomycota diverged and will serve as a guide for future genomic mining projects to discover structurally diverse mushroom-derived polyketides.

TL;DR: In this paper, the effect of exogenous gliotoxin on Aspergillus fumigatus and other fungi has been investigated for its antifungal properties, but little is actually known about the effects of gliotoxins on fungal transformation.

TL;DR: Genotyping of sunflower downy mildew pathogen samples in France demonstrates that multiple introductions have aided the establishment of this pathogen in France, and suggests recombination facilitated by these introductions is driving the emergence of new and endemic races in response to host resistance.

TL;DR: Independent and shared functions of the SKN7-mediated signaling pathway with HSK1 and HOG1 are reported here and it is concluded that SKN 7 is likely involved in resistance to sugar-induced osmotic stress via the HSK 1 signaling pathway.

TL;DR: A novel function for NMRA is proposed, the modulation of AREA and AREB activity in response to the carbon status of the cell, which is most probably dependent on NMRA.

TL;DR: Overall this work suggests that D. septosporum has a veA ortholog that is involved in the control of both developmental and secondary metabolite biosynthetic pathways.

TL;DR: The first successful expression of a FRET (Förster Resonance Energy Transfer)-based Ca(2+) biosensor in fungi is reported, and time-lapse imaging of Magnaporthe oryzae, FUSarium oxysporum, and Fusarium graminearum expressing this sensor showed that instead of a continuous gradient, the cytoplasmic Ca( 2+) change occurred in a pulsatile manner with no discernable gradient between pulses, and each species