Showing papers in "Fungal Genetics and Biology in 2018"

TL;DR: It is demonstrated that by using single-stranded oligo nucleotides for CRISPR-Cas9 mediated gene editing it is possible to introduce specific point mutations as well gene deletions at efficiencies approaching 100% and the efficiency of the system invites for multiplexing.

TL;DR: The intention of the review is to synthesize what is known about ROS metabolism and highlight knowledge gaps that could be hindering the discovery of novel means to mediate beneficial plant- microbe interactions at the expense of harmful plant-microbe interactions.

TL;DR: The mutants generated in this study displayed the strong expected phenotypes, demonstrating this F. oxysporum-optimized CRISPR/Cas9 system is stable and can efficiently disrupt the genes of interest.

TL;DR: Significant in-host microevolution was identified in 13 consecutive A. fumigatus isolates from a single patient promoting fungal persistence and 248 non-synonymous single nucleotide polymorphisms developed throughout series.

TL;DR: Results show for the first time that MAP kinase Slt2 orthologs play similar roles in regulating mycelial growth, conidiation, trap formation, stress resistance, and pathogenicity in the divergent nematode-trapping fungal species A. oligospora and M. haptotylum.

TL;DR: It is shown that creA played an important role in the morphology, pathogenicity and secondary metabolite production of A. flavus, which is responsible for the repression of secondary carbon sources.

TL;DR: Deletion of sntB increased global levels of H3K9K14 acetylation and impaired several developmental processes including sclerotia formation, heterokaryon compatibility, secondary metabolite synthesis, and ability to colonize host seeds; in contrast the overexpression strain displayed fewer phenotypes.

TL;DR: The original plant GB syntax was adapted here to incorporate specific GB structures for gene disruption through homologous recombination and dual selection and the name of FungalBraid is proposed for this new branch of the GB technology that provides open, exchangeable and collaborative resources to the fungal research community.

TL;DR: Cell wall remodeling is an important process during isotropic and polarized growth, characterized by an increase of transcripts coding for hyphal growth and cell cycle/DNA processing when polarized growth is initiated.

TL;DR: Overall, LeDnaJ isolved in fungal growth, T. atroviride resistance, and thermotolerance by regulating the IAA biosynthesis in L. edodes.

TL;DR: Wood-degrading fungi that selectively remove carbohydrates (brown rot) combine Fenton-based oxidation and enzymatic hydrolysis to degrade wood with the plausibility of a protective role for oxalate is demonstrated, to emphasize that these fungi might be optimizing oxalates at a given level to maximize Fenton reactions but to minimize oxidative damage.

TL;DR: A. flavus secondary metabolites produced during infection of maize seed are of particular interest, especially with respect to their roles in the biology of the fungus as mentioned in this paper, which can colonize important food staples and produce aflatoxins, a group of toxic and carcinogenic secondary metabolites.

TL;DR: Notably, D. septosporum has not caused heavy damage to P. sylvestris in northern Europe, which may suggest a long co-existence of the host and the pathogen.

TL;DR: Results indicate that T. arundinaceum TRI6 regulates expression of both tri and mevalonate pathway genes, and it remains to be determined whether the effects of tri6 deletion on expression of other SM clusters resulted because TRI6 can bind to promoter regions of cluster genes or because trichothecene production affects other SM pathways.

TL;DR: Findings suggest that fungal VAOs may have originated from bacterial ancestors, obtained by fungi through horizontal gene transfer, and argue for a more reliable functional prediction using critical amino acid residues.

TL;DR: A downstream target gene of the HOG1 MAPK pathways coding for an aldo-keto reductase, named Bbakr1, was characterized in the insect fungal pathogen, Beauveria bassiana, and displayed increased sensitivity to osmotic/high-salt stress with decreased compatible solute accumulation.

TL;DR: The characteristics of haplotypes distributing along the river demonstrate that the diffusion and modern distribution pattern of species was influenced by river expansion, which is critical for the protection of the diversity of truffles in this region.

TL;DR: The results corroborate that MAT locus idiomorphs are unequally distributed in the majority of the analyzed species and the ability to mate with a partner of the opposite sex is limited to a few zoophilic species and hypothesize that unisexual reproduction is the next evolutionary stage of propagation in dermatophytes which involves the extinction of one mating idiomorph.

TL;DR: Comparisons of mating-type idiomorphs in heterothallic species of Thielaviopsis showed that MAT1-1-2 is involved in fruiting body development, a role that would be unaffected by its idiomorphic position, and this notion is supported by the findings for the MAT1 locus structure in Thielavopsis species.

TL;DR: These findings provide the first evidence that CRISPR/Cas9 can be successfully used in C. orthopsilosis and demonstrate that CoALS4210 plays a direct role in the adhesion of C. Orthopsilotic edited strains to buccal cells.

TL;DR: The findings indicate that a complex PHO network existed in the ancient branches of the fungi, persists in diverse extant fungi, and that this ancient network is likely to be involved in development and cell cycle regulation.

TL;DR: Findings support AfRho1 as an essential regulator of the cell wall integrity, stress response, and pathogenesis of A. fumigatus.

TL;DR: To examine if any parameter of the ΔlaeA phenotype is due to decreased metR expression, an over-expression allele (OE::metR) was placed in a laeA background and could not significantly restore expression of MetR regulated genes in ΔlAEA but did restore sensitivity to sodium selenate.

TL;DR: The findings support the notion that exocytosis and endocytotic operate in tandem with the latter serving as a compensatory process to remove any excess of plasma membrane generated by the intense exocyTosis in the hyphal tips.

TL;DR: The study provides a foundation for comparison of mating type evolution between Botryosphaeriaceae and other fungi and also provides valuable markers for population biology studies in this family.

TL;DR: This work is the first report on the involvement of specific transporters in fungicide uptake and toxicity and their impact on fungal metabolism regulation and results might be further exploited towards the deeper understanding of fungal resistance to fungicides.

TL;DR: To investigate whether individual genes influence the function and expression of other genes, phenotypes and Ca2+ signatures of their double and triple mutants, as well as their expression patterns, were analyzed.

TL;DR: The data indicated that Vph2 and Vma6 were essential for the proper localization of V-ATPase subunits, cell wall functions, filamentous growth and C. albicans pathogenesis, and provided the potential to better exploit V- ATPase-related proteins as antifungal targets.

TL;DR: The results show that molecular triggers for galactose toxicity in U. maydis differ from yeast and mammals, and systematic deletions and double deletions of different genes involved in galactosed metabolism point to a minor role of galactOSE-1-phosphate in galACTose toxicity.

TL;DR: It is shown that these three inhibitors on their own are cytotoxic to fission yeast, suggesting that they have off-targets in vivo except for kinesin-14, and AZ82 can neutralize otherwise toxic overproduced HSET.