Showing papers in "Genomics in 2000"

TL;DR: In this paper, the authors developed models that account for heterogeneous omega ratios among amino acid sites and applied them to phylogenetic analyses of protein-coding DNA sequences, which are useful for testing for adaptive molecular evolution and identifying amino acid points under diversifying selection.

TL;DR: A gene family of small membrane proteins, represented by phospholemman and the gamma subunit of Na,K-ATPase, was defined and characterized by the analysis of more than 1000 related ESTs (expressed sequence tags), named FXYD (pronounced fix-id) in recognition of invariant amino acids in its signature motif.

TL;DR: This paper describes the characterization and cloning of a gene that is a candidate for the tumor-secreted phosphaturic factor and has major similarities to a group of bone-tooth mineral matrix phospho-glycoproteins, including MEPE, which all map to a defined region in chromosome 4q.

TL;DR: The DNMT3L gene that is related to the cytosine-5-methyltransferase 3 (DNMT3) family is isolated and expressed at low levels in several tissues including testis, ovary, and thymus.

TL;DR: The cloning and characterization of three members of the fatty acid desaturase (FADS) gene family in humans are reported and are likely to have arisen evolutionarily from gene duplication as they share a remarkably similar exon/intron organization.

TL;DR: Data suggest that NPC1L1 and NPC1 form part of a family of related proteins that may have similar functions at different subcellular locations, perhaps at sequential steps of the same cholesterol transport pathway.

TL;DR: Fluorescence in situ hybridization and characterization of BAC clones revealed four full-length DAZ genes on the human Y chromosome, and can reconstruct several steps in the evolution of theDAZ genes in the ancestral Y-chromosomal DAZ gene.

TL;DR: It is proposed that a high level of XI AP to XAF1 expression in cancer cells may provide a survival advantage through the relative increase of XIAP anti-apoptotic function.

TL;DR: It is shown that the same Chromosome 10 gene (Ahl) is a major contributor to AHL in nine other inbred mouse strains-129P1/ReJ, A/J, BALB/cByJ, BUB/BnJ, C57BR/cdJ, DBA/2J, NOD/LtJ, SKH2/j, and STOCK760.

TL;DR: GeneChip technology is reasonably reliable for quantitative analysis of expression profiling and would be appropriate as a common platform upon which to build a gene expression database.

TL;DR: The genetic architecture of variation in adult life span was examined for a population of recombinant inbred lines, each of which had been crossed to both inbred parental strains from which the lines were derived, after emergence from both high and low larval density.

TL;DR: Overall the data suggest that DDAH2 may be the more ancient of the two genes, and comparison of the gene structures indicates that the intron/exon organization is highly conserved.

TL;DR: The expansion of the SLC26 family is reported with five new members in chromosomes 3, 6, 8, 12, and 17 and mapping of SLC 26A1 to 4p16.3, which encodes a predicted 738-amino-acid transmembrane protein, and is most abundantly expressed in the kidney and pancreas.

TL;DR: It was concluded that there are multiple NRP1 and NRP2 isoforms including intact and soluble forms, and characterization of these isoforms should help to elucidate the function of NRPs in neuronal guidance and angiogenesis.

TL;DR: A human cDNA sequence corresponding to a novel member of the glucose transporter family was identified (GLUT1-5), and it encodes a putative transporter of 540 amino acids, similar to the other family members.

TL;DR: The data indicate that high-throughput screening of an arrayed protein expression library is an economical way of generating large numbers of clones producing recombinant human proteins for structural and functional analyses.

TL;DR: The present study suggests the contribution of the interleukin 12 B gene or nearby gene(s) to mite-sensitive atopic asthma and a considerable number of genetic variants common across Caucasians and Japanese populations contributing to asthma, although the relative importance of various variants may differ between the groups.

TL;DR: This baboon linkage map is the first reported for any nonhuman primate species and creates opportunities for mapping quantitative trait loci in baboons, as well as for comparative evolutionary analyses of genome structure.

TL;DR: The results demonstrate the existence of additional IL-1 gene family members within the previously definedIL-1 cluster and point to this region of chromosome 2 as an evolutionary hotspot for IL-2 gene duplication and may prove to have an important role in inflammatory responses.

TL;DR: This work has developed a sensitive and rapid flow cytometry-based assay for the multiplexed analysis of SNPs based on polymerase-mediated primer extension, or minisequencing, using microspheres as solid supports, and genomic analysis using multiplexing microsphere arrays (GAMMArrays), enables the simultaneous analysis of dozens, and potentially hundreds ofSNPs per sample.

TL;DR: Findings and the properties of δ-catenin as a neuronal-specific protein, expressed early in development and involved in cell motility, support its role in the mental retardation of CDCS when present in only one copy.

TL;DR: Of the 954 species of 5'UTR, 459 contained no in-frame terminator codon, which is against the common belief, and contrary to the typical translation initiation model, which states that translation is initiated from the "first" ATG codon.

TL;DR: The gene encoding activation-induced cytidine deaminase (AID) was isolated from a murine B cell lymphoma line, CH12F3-2, induced by combined stimulation of TGF-beta, IL-4, and CD40L and RT-PCR analysis of 15 human tissues showed that AID mRNA is expressed strongly in lymph nodes and tonsils.

TL;DR: PMEPA1, along with other highly androgen-induced prostate-specific genes, has potential to serve as an androgen signaling read-out biomarker in prostate tissue.

TL;DR: A novel protein from human cartilage is identified using a subtractive cDNA cloning approach and it is conceivable that the novel protein plays a role in the modulation of FGF receptor activity.

TL;DR: A series of novel bromodomain genes are identified by EST database and cDNA library screening and conservation of multiple domains throughout these genes with Acf1 indicates that they are likely to be components of chromatin remodeling complexes.

TL;DR: Transient transfection assays using a mPer1-luciferase fusion gene revealed that each of the conserved E-boxes additively functions as an enhancer for the transactivation of m per1 by mCLOCK and mBMAL1.

TL;DR: The cloning and characterization of the mouse homologue of human TLR5 is described and it is shown that Tlr5 is expressed predominantly in liver and lung with low-level expression in most other tissues examined, suggesting that, as with other members of this family, TLR5 may play a role in host response to bacterial gram-negative infections.