Showing papers in "Molecular and Biochemical Parasitology in 1988"

TL;DR: A monoclonal antibody raised against a rhoptry enriched subcellular fraction of Toxoplasma gondii tachyzoites reacted in immunofluorescence studies with rod-like organelles located in the anterior part of the organisms and gave specific labeling of rhoptries in immunoelectron microscopy.

TL;DR: From comparisons of the Plasmodium genes to that of humans, it was possible to design genus-specific as well as species-specific oligonucleotide probes that can be used to distinguish the parasite 18S ribosomal RNA from that of its host.

TL;DR: It is found that HeLa cell nuclear extracts are able to accurately splice a Toxoplasma pre-mRNA construct and Trans-splicing, as demonstrated in the Kinetoplastida, is not involved in the formation of the mature α-tubulin transcript in T. gondii.

TL;DR: An immunodominant species-specific surface glycoprotein antigen was purified from procyclic culture forms of Trypanosoma brucei rhodesiense using lectin affinity chromatography and a monoclonal antibody immunoadsorbent, revealing an unusual acidic polypeptide with an amino-terminal amino acid sequence which matched portions of previously published sequences predicted from two different cDNAs obtained using mRNA from pro cyclic trypanosomes.

TL;DR: Nucleotide sequence analysis showed that seven out of the nine isolated clones code for antigens which contain tandemly repeated amino acid sequence motifs which are shared between different strains of T. cruzi.

TL;DR: The codon frequencies used in 7874 codons from 17 sequences of Plasmodium falciparum have been examined, finding that A and C occur with similar frequency in all positions but G is predominantly in the first base and T in the last position.

TL;DR: Monoclonal antibodies designated 8G10/48 and 9E3/48 raised against mature asexual blood stages of Plasmodium falciparum inhibit parasite growth in vitro.

TL;DR: It is concluded that Pf EMP 1 is either directly responsible for the cytoadherence phenomenon, or is very closely associated with another as yet unidentified functional molecule that must have a structural property/function that is important under the host cell membrane.

TL;DR: Several lines of evidence indicate that this acid protease activity is expressed by the major surface glycoprotein (gp63) of L. m.

TL;DR: The abundance of conserved minirepeat sequences containing invariant restriction sites in T. cruzi cells may prove valuable for the development of new direct diagnostic methods for Chagas' disease based on DNA probe technology.

TL;DR: Six strains of those Leishmania species less sensitive to the azole drugs could be subcultured indefinitely at reduced growth rates in the presence of a ketoconazole concentration causing the same extraordinary alterations in sterol composition, suggesting that the bulk membrane functions of sterols in leishmanias can be served by 14 alpha-methyl sterols and cholesterol, albeit imperfectly, while traces of 14alpha-des methyl sterols are needed for uncharacterized metabolic functions.

TL;DR: It is shown, by cDNA nucleotide sequence analysis, that this antigen gene encodes a 989 amino acid protein that contains a potential signal peptide, but not a membrane anchor domain, and is proposed to identity by the acronym SERA, serine-repeat antigen based on its complete structure.

TL;DR: Variation between different geographic isolates of P. falciparum in their dependency on NeuNAc for invasion of host erythrocytes is reported and it is illustrated that the negatively charged COOH group of NeUNAc is not the important group in the interaction of the merozoite with the Neu NAc.

TL;DR: In contrast to oral infection, intraperitoneal exposure of rats with living parasites induced a strong response to both ES and somatic antigen preparations, which might have application in the estimation of the age and degree of penetration by the nematodes in human infection.

TL;DR: Adult Onchocerca volvulus was isolated from nodules removed from onchocerciasis patients at four locations--two in the West African Sudan-savanna region (near Bamako, Mali, and Touboro, Cameroon), one in a West African forest region (Kumba, Cameroon) and one near Guatemala City, Guatemala.

TL;DR: A variant cell line of Leishmania donovani (named R2D2) has been selected for resistance to the cytotoxic lectin ricin agglutinin and shown to be defective in the synthesis of its major glycoconjugate lipophosphoglycan.

TL;DR: The tissue-invasive infective and lung-stage larvae of the nematode Ascaris suum were found to release proteinases during culture in vitro, which contained multiple proteolytic enzyme activities, as defined by pH optima, substrate specificities, and inhibitor profiles.

TL;DR: Four monoclonal antibodies recognise an antigen localised in the rhoptries of Plasmodium falciparum merozoites using both indirect immunofluorescence assay and immunoelectron microscopy with immunogold labeling.

TL;DR: Pulse chase analysis of [35S]methionine biosynthetic labeling of P. falciparum culture demonstrated that the 240 kDa molecule was the precursor of the 225 kDa and that its processing occurred between 0 and 4 h after synthesis.

TL;DR: The NH2-terminal amino acid sequence of the Mr 26 000 glutathione S-transferase of Schistosoma japonicum has been deduced by RNA and protein sequence analysis and a bacterial plasmid has been constructed that directs the synthesis of the entire Sj26 molecule in Escherichia coli.

TL;DR: Evidence is presented that the genome of this human malaria parasite encodes for still another actin protein (pf-actin II), and the amino acid sequence of this protein is very diverged from the actins known thus far.

TL;DR: In situ hybridisation shows that the eggshell precursor gene is only transcribed in immature vitelline cells and has a short half-life, and observations provide persuasive evidence that the 0.9 kb mRNA codes for an eggshell precursors.

TL;DR: Electron microscopic examination of thin sections of parasites immunolabeled with these monoclonal antibodies and colloidal gold anti-mouse conjugate has confirmed that this antigen is localised in the rhoptry organelles of mature schizonts and free merozoites.

TL;DR: A cDNA library derived from mRNA of metacestodes from Echinococcus multilocularis was constructed in the Escherichia coli expression vector lambda gt11 and screened with human patients' sera suggesting the usefulness of the recombinant II/3-antigen for immunodiagnosis of human alveolar echinococcosis.

TL;DR: Constitutively expressed schistosome homologues of heat-shock protein Hsp70 elicit a dominant antibody response in humans infected with either Schistosoma japonicum or Schistosa mansoni; in each case the parasite antigens are immunologically distinct and noncrossreactive.

TL;DR: Variable sequences fall into two distinct types, indicating that the PMMSA is encoded by dimorphic alleles that undergo recombination within conserved blocks at the 5' end of the gene.

TL;DR: The nucleotide sequence of one 936 bp fragment of the P. falciparum molecule was determined and identified, by a data base homology search, as part of a mitochondrial small rRNA subunit, thus confirming the mitochondrial origin of the circular DNAs of both malarial species.

TL;DR: Comparison of RFLPs with monoclonal antibody profiles suggested that neither technique yet provides discrimination between all the isolates which may comprise a strain.

TL;DR: Three oxidant defense enzymes in early and late intraerythrocytic stages of the human malarial parasite, Plasmodium falciparum, are measured and characterized, suggesting some dependence of the parasite on host SOD.

TL;DR: Parasmodium-induced changes in the kinetics of cellular uptake of the nucleosides and in the appearance in infected cells of a nucleoside permeation route of low sensitivity to the classical inhibitor of erythrocytic nucleosid transport, nitrobenzylthioinosine (NBMPR) are reported.