Showing papers in "Molecular Immunology in 2000"

TL;DR: The Jak-STAT pathway is one of the important signaling pathways downstream of cytokine receptors as discussed by the authors, and the recent discovery of the CIS/SOCS/JAB/SSI family of inhibitors has contributed to understanding how this pathway is negatively regulated.

TL;DR: The IgM-type antibodies differ from other classes of antibodies in that they are predominantly produced by B1 cells, in the absence of apparent stimulation by specific antigens.

TL;DR: Tunated glycoforms of an intact IgG1 anti-MHC Class II antibody are shown to exhibit differential functional activity for FcgammaRI and C1 ligands, relative to deglycosylated IgG 1.

TL;DR: A large (152) set of Lama glama VHH cDNAs is described and new VHH-characteristic residues at positions forming interdomain sites in conventional VH domains are identified.

TL;DR: The cloning of a novel human gene encoding the putative orphan receptor, C5L2, belonging to a subfamily of C3a, C 5a and formyl Met-Leu-Ph receptors that are related to the chemokine receptor family is reported.

TL;DR: The difference in sensitivity to various inhibitors and the influence of high ionic strength on the complexes indicate that the activation and control of the MBL pathway differ from that of the classical pathway.

TL;DR: To determine the site(s) responsible for complement activation in anti-NIP-mouse/human IgG3 antibodies, mutated amino acids Lys 276, Tyr 278, Asp 280, Glu 318, Lys 320 and Lys 322 in two beta-strands in the C(H)2 domains of human IgG 3.

TL;DR: This review, which is focused on human IgD but integrates data in the mouse and other species when needed, summarizes present data on the structure, synthesis and functions of both membrane and secIgD, IgD receptors and the involvement of IgD in various diseases, especially the hyperIg D syndrome.

TL;DR: The interaction between 2B4 on effector cells and CD48 on target cells induced NK-cell activation, as evidenced by increased cytotoxicity and secretion of IFN-gamma, demonstrating that activation signals delivered via 1B4 can be regulated by the action of certain inhibitory receptors.

TL;DR: Data confirm the findings of Glenn and colleagues (1998), who showed TCI using cholera toxin can elicit high levels of serum IgG to both the toxin and co-administered antigen and demonstrates that this route of immunization is particularly effective at eliciting humoral immunity in saliva and in the female reproductive tract.

TL;DR: The presence of CD40 on the surface of carcinoma lines was found to be an important factor in the generation of tumour-specific T cell responses.

TL;DR: Comparisons of the basic structure of the human TRAF5 and TRAF6 genes obtained by analysis of the genomic DNA database revealed the evolutionary relationship between the six known mammalian TRAFs, and the Drosophila genome was found to contain three TRAF family genes: dTRAF1, dT RAF6 and a previously undescribed member the authors have designated dT TRAF3.

TL;DR: An easy way to increase the functional affinity (avidity) of single chain variable fragments (scFvs) by tetramerization on streptavidin, following their site-specific biotinylation by the enzyme BirA is described.

TL;DR: New immunoglobulin (Ig) expression vectors are described and it is shown that secretion of recombinant Ig can reach 1.2 microg/ml per million cells per day for transfected B cells.

TL;DR: The new approach using the site-specific cleavage with two IgA1 proteases allowed precise and sensitive MALDI-TOF mass spectrometric analysis of O-glycosylation heterogeneity in IgA 1 hinge region.

TL;DR: The effect of somatic hypermutation on potential sites of N-glycosylation in IgV(H) genes used by mucosal plasma cells secreting IgM, IgA and IgG in adults and children is studied.

TL;DR: The first marsupial homologues of the Fc receptor heavy and light chains, FcRn and beta-2 microglobulin, from the brushtail possum have been cloned and characterised and suggest that expression of theFc receptor in the mammary gland is controlled by the expression of beta- 2 microglOBulin and that its expression is upregulated during the period of highest IgG-transfer into milk.

TL;DR: V(D)J recombination frequencies and the quality of signal and coding joining are comparable to wild-type controls, as analyzed by a cellular V(D),J recombinations assay.

TL;DR: Results show that the humanized KM8969, having the optimized immune effector functions and theoretically minimal immunogenicity, is an ideal candidate to test the effectiveness of anti-GM2 MAb in human cancer therapy.

TL;DR: Monoclonal antibodies are developed that specifically inhibit alternative pathway function and suggest that the alternative pathway contributes significantly to the formation of complement activation products in blood when the classical pathway is initially triggered.

TL;DR: The analysis indicates that retrotransposition as well as single and multiple exon duplication events are likely to have been involved in SCR radiation and RCA gene evolution, facilitated by conservation of splice-phasing and the single-exon, single-SCR nature of the encoded domains.

TL;DR: The data suggests that increases in intracellular calcium induce the activation of ERK1 and 2 in human T lymphocytes via sequential activation of CaM-kinase and phosphorylation of p56(Lck).

TL;DR: Results suggested that the stoichiometry depended largely on the size of the Ag involved and that mAbs with a low affinity are more efficient at raising the binding strength through divalent interaction since the avidity of two mAbs in interactions with highly haptenated BSA was not significantly different in spite of a 10-fold difference in affinity to the monovalent NP(1)-HEL.

TL;DR: Serine phosphorylation of STAT6 was resistant to H7 and HA1004, inhibitors of many serine/threonine kinases including PKC, and coupled with previous studies showing that IL-4 does not activate MAPK pathways in lymphocytes, suggest thatIL-4 may induce serine phosphories in STAT6 by a novel-signaling pathway.

TL;DR: Computer-generated three-dimensional models provided the structural rationale for these observed effects in MHC binding and T cell responses resulted from the modifications in the AFP(542-550) sequence.

TL;DR: Two lines of evidence are reported demonstrating that this inducible lymphocyte response is genetically programmed by transcription factor NF-kappaB and contingent upon proteolytic breakdown of its cytoplasmic inhibitor IkappaBalpha.

TL;DR: The data indicate that nibrin plays no essential role in V(D)J recombination and is not required for the generation of an apparently diverse B cell repertoire.

TL;DR: It is interpreted to mean that in vivo gene conversion in this species can only be unambiguously proven when the VDJs from individual B-cells are bearing a single VDJ rearrangement amplified and sequenced or when V DJs are cloned without the use of PCR.

TL;DR: The results suggest that binding of a ligand to HveA may alter the conformation of this receptor, thereby affecting its interaction with its other ligands.

TL;DR: The relative ability of rCte f 1 to bind IgE in the serum of flea allergic animals, produced in these three expression systems, matched that of the native allergen.