Journal ArticleDOI
3D-Bioprinted Difunctional Scaffold for In Situ Cartilage Regeneration Based on Aptamer-Directed Cell Recruitment and Growth Factor-Enhanced Cell Chondrogenesis.
Zhen Yang,Zhen Yang,Tianyuan Zhao,Tianyuan Zhao,Cangjian Gao,Cangjian Gao,Fuyang Cao,Fuyang Cao,Hao Li,Hao Li,Zhiyao Liao,Zhiyao Liao,Liwei Fu,Liwei Fu,Pinxue Li,Pinxue Li,Wei Chen,Wei Chen,Zhiqiang Sun,Zhiqiang Sun,Shuangpeng Jiang,Zhuang Tian,Guangzhao Tian,Guangzhao Tian,Kangkang Zha,Kangkang Zha,Tingting Pan,Xu Li,Xiang Sui,Zhiguo Yuan,Shuyun Liu,Quanyi Guo,Quanyi Guo +32 more
TLDR
In this article, a 3D-bioprinted difunctional scaffold was developed based on aptamer HM69-mediated MSC-specific recruitment and growth factor-enhanced cell chondrogenesis.Abstract:
Articular cartilage (AC) lesions are fairly common but remain an obstacle for clinicians and researchers due to their poor self-healing capacity. Recently, a promising therapy based on the recruitment of autologous mesenchymal stem cells (MSCs) has been developed for the regeneration of full-thickness cartilage defects in the knee joint. In this study, a 3D-bioprinted difunctional scaffold was developed based on aptamer HM69-mediated MSC-specific recruitment and growth factor-enhanced cell chondrogenesis. The aptamer, which can specifically recognize and recruit MSCs, was first chemically conjugated to the decellularized cartilage extracellular matrix and then mixed with gelatin methacrylate to form a photocrosslinkable bioink ready for 3D bioprinting. Together with the growth factor that promoted cell chondrogenic differentiation, the biodegradable polymer poly(e-caprolactone) was further chosen to impart mechanical strength to the 3D bioprinted constructs. The difunctional scaffold specifically recruited MSCs, provided a favorable microenvironment for cell adhesion and proliferation, promoted chondrogenesis, and thus greatly improved cartilage repair in rabbit full-thickness defects. In conclusion, this study demonstrated that 3D bioprinting of difunctional scaffolds could be a promising strategy for in situ AC regeneration based on aptamer-directed cell recruitment and growth-factor-enhanced cell chondrogenesis.read more
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Journal ArticleDOI
Chitosan hydrogel/3D-printed poly(ε-caprolactone) hybrid scaffold containing synovial mesenchymal stem cells for cartilage regeneration based on tetrahedral framework nucleic acid recruitment.
Pinxue Li,Liwei Fu,Zhiyao Liao,Yu Peng,Chao Ning,Cangjian Gao,Daxu Zhang,Xiang Sui,Yunfeng Lin,Shuyun Liu,Chunxiang Hao,Quanyi Guo +11 more
TL;DR: In this article, a cartilage regenerative system was developed based on a chitosan (CS) hydrogel/3D-printed poly(e-caprolactone) (PCL) hybrid containing synovial MSCs and recruiting tetrahedral framework nucleic acid (TFNA) injected into the articular cavity.
Journal ArticleDOI
Recent advances in hyaluronic acid-based hydrogels for 3D bioprinting in tissue engineering applications
TL;DR: In this article , the authors discuss the strategies adopted for the application of hyaluronic acid-based hydrogels as bioinks, including printability, improving their mechanical properties, and printing with loaded cells.
Journal ArticleDOI
Advances in Translational 3D Printing for Cartilage, Bone, and Osteochondral Tissue Engineering.
TL;DR: This review outlines the recently developed 3D printing techniques for clinical translation and specifically summarizes the applications of these approaches for the regeneration of cartilage, bone, and osteochondral tissues.
Journal ArticleDOI
Advances of Hydrogel-Based Bioprinting for Cartilage Tissue Engineering.
TL;DR: The opportunities and challenges of 3D bioprinting technique to construct complex bio-inks with adjustable mechanical and biological integrity, and meanwhile, the current possible solutions are also conducted for providing some suggestive ideas on developing more advanced biopprinting products from the bench to the clinic.
Journal ArticleDOI
Recent Advances of Self-Healing Polymer Materials via Supramolecular Forces for Biomedical Applications.
TL;DR: The different categories of supramolecular forces used in preparing self-healing materials are introduced and biological applications developed in the last 5 years are described, including antibiofouling, smart drug/protein delivery, wound healing, electronic skin, cartilage lubrication protection, and tissue engineering scaffolds.
References
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A cartilage ECM-derived 3-D porous acellular matrix scaffold for in vivo cartilage tissue engineering with PKH26-labeled chondrogenic bone marrow-derived mesenchymal stem cells
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Journal ArticleDOI
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TL;DR: A brief outlook on the promises, strategies, and current applications of endogenous stem cell homing for in situ tissue regeneration, with particular emphasis placed upon pharmacological means based on cell-instructive scaffolds and release technology to direct cell mobilization and recruitment.
Journal ArticleDOI
A comparison of different bioinks for 3D bioprinting of fibrocartilage and hyaline cartilage
TL;DR: This study demonstrates the importance of the choice of bioink when bioprinting different cartilaginous tissues for musculoskeletal applications and demonstrates that it is possible to engineer mechanically reinforced hydrogels with high cell viability by co-depositing a hydrogel bioink with polycaprolactone filaments.