A biochemical study of the intermediary carbon metabolism of Shewanella putrefaciens.
J H Scott,Kenneth H. Nealson +1 more
TLDR
In this paper, cell extracts were used to determine the enzymes involved in the intermediary carbon metabolism of several strains of Shewanella putrefaciens, including the Entner-Doudoroff pathway and the Embden-Meyerhof-Parnas pathway.Abstract:
Cell extracts were used to determine the enzymes involved in the intermediary carbon metabolism of several strains of Shewanella putrefaciens. Enzymes of the Entner-Doudoroff pathway (6-phosphogluconate dehydratase and 2-keto-3-deoxy-6-phosphogluconate aldolase) were detected, but those of the Embden-Meyerhof-Parnas pathway were not. While several tricarboxylic acid cycle enzymes were present under both aerobic and anaerobic conditions, two key enzymes (2-oxoglutarate dehydrogenase and pyruvate dehydrogenase) were greatly diminished under anaerobic conditions. Extracts of cell grown anaerobically on formate as the sole source of carbon and energy were positive for hydroxypyruvate reductase, the key enzyme of the serine pathway in other methylotrophs, while no hexulose synthase activity was seen.read more
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Book ChapterDOI
Dissimilatory Fe(III) and Mn(IV) reduction.
TL;DR: The ability to oxidize hydrogen with the reduction of Fe(III) is a highly conserved characteristic of hyperthermophilic microorganisms, most notably those in the Geobacteraceae family as mentioned in this paper.
Journal ArticleDOI
Towards environmental systems biology of Shewanella
James K. Fredrickson,Margaret F. Romine,Alexander S. Beliaev,Jennifer M. Auchtung,Michael E. Driscoll,Timothy S. Gardner,Kenneth H. Nealson,Andrei L. Osterman,Grigoriy E. Pinchuk,Jennifer L. Reed,Dmitry A. Rodionov,Jorge L. M. Rodrigues,Daad A. Saffarini,Margrethe H. Serres,Alfred M. Spormann,Igor B. Zhulin,James M. Tiedje +16 more
TL;DR: Systems-level analysis of the model species Shewanella oneidensis MR-1 and other members of this genus has provided new insights into the signal-transduction proteins, regulators, and metabolic and respiratory subsystems that govern the remarkable versatility of the shewanellae.
Journal ArticleDOI
Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis.
John F. Heidelberg,Ian T. Paulsen,Karen E. Nelson,Eric Gaidos,Eric Gaidos,William C. Nelson,Timothy D. Read,Jonathan A. Eisen,Rekha Seshadri,Naomi L. Ward,Barbara A. Methé,Rebecca A. Clayton,Terry E. Meyer,A. I. Tsapin,James Scott,Maureen J. Beanan,Lauren M. Brinkac,Sean C. Daugherty,Robert T. DeBoy,Robert J. Dodson,A. Scott Durkin,Daniel H. Haft,James F. Kolonay,Ramana Madupu,Jeremy Peterson,Lowell Umayam,Owen White,Alex M. Wolf,Jessica Vamathevan,Janice Weidman,Marjorie Impraim,K. Lee,Kristy Berry,Chris Lee,Jacob L. Mueller,Hoda Khouri,John Gill,T. Utterback,Lisa McDonald,Tamara Feldblyum,Hamilton O. Smith,J. Craig Venter,Kenneth H. Nealson,Kenneth H. Nealson,Claire M. Fraser +44 more
TL;DR: This genome sequence represents a critical step in the elucidation of the pathways for reduction (and bioremediation) of pollutants such as uranium (U) and chromium (Cr), and offers a starting point for defining this organism's complex electron transport systems and metal ion–reducing capabilities.
Journal ArticleDOI
Fractionation of Carbon and Hydrogen Isotopes in Biosynthetic Processes
TL;DR: In this article, a review of the isotopic relationships between organic compounds produced by a single organism, specifically their enrichments or depletions in 13C relative to total-biomass carbon, is presented.
Journal ArticleDOI
Isolation of the Exoelectrogenic Bacterium Ochrobactrum anthropi YZ-1 by Using a U-Tube Microbial Fuel Cell
TL;DR: A special U-tube-shaped MFC was developed to enrich exoelectrogenic bacteria with isolation based on dilution-to-extinction methods, and a pure culture identified as Ochrobactrum anthropi YZ-1 was obtained based on 16S rRNA gene sequencing and physiological and biochemical characterization.
References
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Journal ArticleDOI
Bacterial Manganese Reduction and Growth with Manganese Oxide as the Sole Electron Acceptor
TL;DR: The characteristics of this reduction are consistent with a biological, and not an indirect chemical, reduction of manganese, which suggest that this bacterium uses manganic oxide as a terminal electron acceptor.
Journal ArticleDOI
Phylogeny of the Vibrionaceae, and recommendation for two new genera, Listonella and Shewanella
M.T. MacDonell,Rita R. Colwell +1 more
TL;DR: Results of this study indicate a good correlation between the taxonomy of the Vibrionaceae established using conventional and molecular genetic data and evolutionary relationships deduced from 5S rRNA sequences, however, several species require reconsideration of their taxonomic status, based on the new information obtained in this study.
Journal ArticleDOI
Complete oxidation of solid phase sulfides by manganese and bacteria in anoxic marine sediments
Robert C. Aller,Peter D. Rude +1 more
TL;DR: In this article, it was shown that in natural sediments Mn-oxides can completely oxidize solid phase sulfides to SO4− under anoxic conditions and that the reaction is inhibited by DNP (dinitrophenol) and azide, implying biological mediation by a group of chemolithotrophic bacteria.
Microbial reduction of manganese and iron: New approaches to carbon cycling. [Shewanella putrefaciens]
K.H. Nealson,C.R. Myers +1 more
TL;DR: This brief review introduces the reader to the importance and conceptual aspects of microbial metal reduction by focusing on a single group of Mn(IV)- and Fe(III)-reducing organisms in the group Shewanella putrefaciens.
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