Journal ArticleDOI
A comparison of the properties of Sox-3 with Sry and two related genes, Sox-1 and Sox-2
Jérôme Collignon,Shanthini Sockanathan,Adam Hacker,Michel Cohen-Tannoudji,Dominic Norris,Sohaila Rastan,Milena Stevanovic,Peter N. Goodfellow,Robin Lovell-Badge +8 more
Reads0
Chats0
TLDR
It is demonstrated that Sox-3, as well as Sox-1 and Sox-2, are expressed in the urogenital ridge and that their protein products are able to bind the same DNA sequence motif as Sry in vitro, but with different affinities, suggesting an evolutionary link between the genes.Abstract:
The Sox gene family consists of a large number of embryonically expressed genes related via the possession of a 79-amino-acid DNA-binding domain known as the HMG box Partial clones for the first three Sox genes (al-a3) were isolated by homology to the HMG box of the testis-determining gene Sry and are now termed Sox-1, Sox-2 and Sox-3, Sox-3 is highly conserved amongst mammalian species and is located on the X chromosome This has led to the proposal that Sry evolved from Sox-3 We present the cloning and sequencing of Sox-1, Sox-2 and Sox-3 from the mouse and show that Sox-3 is most closely relate to Sry We also confirm that mouse Sox-3 is located on the X chromosome between Hprt and Dmd Analysis of the distribution of Sox-3 RNA shows that its main site of expression is in the developing central nervous system, suggesting a role for Sox-3 in neural development Moreover, we demonstrate that Sox-3, as well as Sox-1 and Sox-2, are expressed in the urogenital ridge and that their protein products are able to bind the same DNA sequence motif as Sry in vitro, but with different affinities These observations prompt discussion of an evolutionary link between the genes and support the model that Sry has evolved from Sox-3 However our findings imply that if this is true, then Sry has undergone concomitant changes resulting in loss of CNS expression and altered DNA-binding propertiesread more
Citations
More filters
Journal ArticleDOI
Multipotent cell lineages in early mouse development depend on SOX2 function
Ariel A. Avilion,Silvia K. Nicolis,Larysa H. Pevny,Lidia Pérez,Nigel Vivian,Robin Lovell-Badge +5 more
TL;DR: The data suggest that maternal components could be involved in establishing early cell fate decisions and that a combinatorial code, requiring SOX2 and OCT4, specifies the first three lineages present at implantation.
Journal ArticleDOI
Pten is essential for embryonic development and tumour suppression.
TL;DR: The notion that PTEN haploinsufficiency plays a causal role in CD, LDD and BZS pathogenesis is supported, and it is demonstrated that Pten is a tumour suppressor essential for embryonic development.
Journal ArticleDOI
SOX2 Functions to Maintain Neural Progenitor Identity
TL;DR: It is shown here that constitutive expression of SOX2 inhibits neuronal differentiation and results in the maintenance of progenitor characteristics, and that SOXB1 signaling is both necessary and sufficient to maintain panneural properties of neural progenitors.
Journal ArticleDOI
Phylogeny of the SOX family of developmental transcription factors based on sequence and structural indicators.
TL;DR: A robust phylogeny of SOX genes is proposed that reflects their evolutionary history in metazoans and finds support for subdivision of the family into groups A-H, as has been suggested in some previous studies, and for the assignment of two new groups, I and J.
Journal ArticleDOI
From head to toes: The multiple facets of Sox proteins
TL;DR: Sox proteins perform their function in a complex interplay with other transcription factors in a manner highly dependent on cell type and promoter context, and exhibit a remarkable crosstalk and functional redundancy among each other.
References
More filters
Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Journal ArticleDOI
A comprehensive set of sequence analysis programs for the VAX
TL;DR: A group of programs that will interact with each other has been developed for the Digital Equipment Corporation VAX computer using the VMS operating system.
Journal ArticleDOI
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
D B Smith,Kevin S. Johnson +1 more
TL;DR: Plasmid expression vectors have been constructed that direct the synthesis of foreign polypeptides in Escherichia coli as fusions with the C terminus of Sj26, a 26-kDa glutathione S-transferase (GST; EC 2.5.1.18) encoded by the parasitic helminth Schistosoma japonicum.
Journal ArticleDOI
A gene from the human sex-determining region encodes a protein with homology to a conserved DNA-binding motif
Andrew H. Sinclair,Berta P,Berta P,M. S. Palmer,Hawkins,B. Griffiths,Smith Mj,Foster Jw,Foster Jw,A.-M. Frischauf,Robin Lovell-Badge,Peter N. Goodfellow +11 more
TL;DR: A search of a 35-kilobase region of the human Y chromosome necessary for male sex determination has resulted in the identification of a new gene, termed SRY (for sex-determining region Y) and proposed to be a candidate for the elusive testis-d determining gene, TDF.
Journal ArticleDOI
Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
Charles Auffray,François Rougeon +1 more
TL;DR: A procedure is described for the large-scale purification of light (L) and heavy (H) chain mRNAs from plasmacytomas produced in mice by oligo(dT)-cellulose chromatography and either sucrose gradient centrifugation in conditions preventing aggregation or by means of high-resolution preparative gel electrophoresis under non-denaturing conditions.