A detailed study of the periodate oxidation of sialic acids in glycoproteins.
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Periodate oxidation of terminalN-acetyl- andN-glycoloylneuraminic acid residues in the mucins from edible bird nest substance and pig submandibular gland can be carried out under conditions which exclusively give rise to the formation of the C-7 analogues of these sialic acids.Abstract:
Periodate oxidation of terminalN-acetyl- andN-glycoloylneuraminic acid residues in the mucins from edible bird nest substance and pig submandibular gland, respectively, can be carried out under conditions which exclusively give rise to the formation of the C-7 analogues of these sialic acids. In contrast, the C-8 compounds can be obtained in a maximum yield of about 40%. Under identical conditions,N-glycoloylneuraminic acid is oxidized about 1.5 times faster than theN-acetylated derivative. After release of the sialic acids by acid hydrolysis, the characterization of the oxidation products was carried out by TLC, by GLC and GLC-MS of the corresponding pertrimethylsilyl derivatives, and by 500-MHz1H-NMR spectroscopy. In addition, molar response factors for GLC analysis and extinction coefficients in the orcinol/Fe3+/HCl assay were determined.read more
Citations
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Journal ArticleDOI
P-type lectins.
TL;DR: The I- type lectins are a subset of the immunoglobulin superfamily that are capable of carbohydrate-protein interactions and there are I-type lectins recognizing sialic acids, other sugars and glycosaminoglycans.
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Chapter 11 – Chemistry, biochemistry and biology of sialic acids ☆
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Immunogenicity in animals of a polysaccharide-protein conjugate vaccine against type III group B Streptococcus.
Michael R. Wessels,Lawrence C. Paoletti,Dennis L. Kasper,J L DiFabio,F Michon,K Holme,Harold J. Jennings +6 more
TL;DR: The results suggest that this method of conjugation to a carrier protein may be a useful strategy to improve the immunogenicity of the type III group B Streptococcus polysaccharide in human subjects.
Book ChapterDOI
Exploration of the Sialic Acid World.
TL;DR: Sialidases (neuraminidases), which liberate sialic acids from cellular compounds, had been known from very early on from studies with influenza viruses, and are studied because of their significance in development and, for instance, in cancer.
Journal ArticleDOI
I-type lectins.
TL;DR: The I- type lectins are a subset of the immunoglobulin superfamily that are capable of carbohydrate-protein interactions and there are I-type lectins recognizing sialic acids, other sugars and glycosaminoglycans.
References
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Journal ArticleDOI
The role of membrane sialyl and galactosyl residues in regulation of the life-time of rabbit erythrocytes.
Book ChapterDOI
Colorimetry and Thin-Layer Chromatography of Sialic Acids
TL;DR: Colorimetry provides a routine and accurate assay for sialic acids if properly controlled and cases may arise where purification is still required (e.g. the assay of tissue and cell homogenates).
Journal ArticleDOI
Sialoglycoproteins and sialic acids of plasmodium knowlesi schizont-infected erythrocytes and normal rhesus monkey erythrocytes
TL;DR: The effects of malaria infection on RBC sialic acids and sialoglycoproteins were studied with asexual blood-stage infections of Plasmodium knowlesi in rhesus monkeys and found that infected RBC infected with ring-stage P. knowlesi had the same content as normal RBC.
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