A multi-signal fluorescent probe for simultaneously distinguishing and sequentially sensing cysteine/homocysteine, glutathione, and hydrogen sulfide in living cells
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TLDR
A multi-signal fluorescent probe was engineered for simultaneously distinguishing and sequentially sensing cysteine/homocysteine, glutathione, and hydrogen sulfide in living cells.Abstract:
Biothiols, which have a close network of generation and metabolic pathways among them, are essential reactive sulfur species (RSS) in the cells and play vital roles in human physiology. However, biothiols possess highly similar chemical structures and properties, resulting in it being an enormous challenge to simultaneously discriminate them from each other. Herein, we develop a unique fluorescent probe (HMN) for not only simultaneously distinguishing Cys/Hcy, GSH, and H2S from each other, but also sequentially sensing Cys/Hcy/GSH and H2S using a multi-channel fluorescence mode for the first time. When responding to the respective biothiols, the robust probe exhibits multiple sets of fluorescence signals at three distinct emission bands (blue-green-red). The new probe can also sense H2S at different concentration levels with changes of fluorescence at the blue and red emission bands. In addition, the novel probe HMN is able to discriminate and sequentially sense biothiols in biological environments via three-color fluorescence imaging. We expect that the development of the robust probe HMN will provide a powerful strategy to design fluorescent probes for the discrimination and sequential detection of biothiols, and offer a promising tool for exploring the interrelated roles of biothiols in various physiological and pathological conditions.read more
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TL;DR: This review focuses on recent innovations in probe design, detection mechanisms and the investigation of biological processes and the ongoing development of fluorescent probes to enable deeper insight into the physiology of bioactive analytes.
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A Multi-signal Fluorescent Probe with Multiple Binding Sites for Simultaneous Sensing of Cysteine, Homocysteine, and Glutathione.
Guoxing Yin,Tingting Niu,Yabing Gan,Ting Yu,Peng Yin,Haimin Chen,Youyu Zhang,Haitao Li,Shouzhuo Yao +8 more
TL;DR: A novel fluorescent probe developed by integrating chlorinated coumarin and benzothiazolylacetonitrile and exploited for simultaneous detection of cysteine, homocysteine (Hcy), and glutathione (GSH) exhibited rapid fluorescence turn-on for distinguishing Cys, Hcy, and GSH with 108-, 128-, 30-fold fluorescence increases at 457, 559, 529 nm, respectively.
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Simultaneous Visualization of Endogenous Homocysteine, Cysteine, Glutathione, and their Transformation through Different Fluorescence Channels
Guoxing Yin,Tingting Niu,Ting Yu,Yabing Gan,Sun Xinyu,Peng Yin,Haimin Chen,Youyu Zhang,Haitao Li,Shouzhuo Yao +9 more
TL;DR: A novel multi-signal fluorescent probe was rationally designed and exploited for the simultaneous sensing of homocysteine, cysteine (Cys), and glutathione (GSH) using different emission channels and their transformation in living cells.
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Recent advances in the development of responsive probes for selective detection of cysteine
TL;DR: In this review, advances in the development of Cys-responsive luminescence probes, including molecular probes and nanoprobes, are included and discussed in sections corresponding to their response mechanisms.
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