A novel bifunctional wax ester synthase/acyl-CoA:diacylglycerol acyltransferase mediates wax ester and triacylglycerol biosynthesis in Acinetobacter calcoaceticus ADP1.
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TLDR
The identification of a bifunctional enzyme from this bacterium exhibiting acyl-CoA:fatty alcohol acyltransferase (wax ester synthase, WS) as well as acyl/DGAT activity that could establish novel processes for biotechnological production of jojoba-like wax esters is described.About:
This article is published in Journal of Biological Chemistry.The article was published on 2003-03-07 and is currently open access. It has received 431 citations till now. The article focuses on the topics: Acyltransferase & Wax ester.read more
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Biodiesel production from oleaginous microorganisms
TL;DR: Regulation mechanism of oil accumulation in microorganism and approach of making microbial diesel economically competitive with petrodiesel are discussed in this review.
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Thematic review series: glycerolipids. DGAT enzymes and triacylglycerol biosynthesis.
TL;DR: The genes encoding two DGAT enzymes, DGAT1 and DGAT2, were identified in the past decade, and the use of molecular tools, including mice deficient in either enzyme, has shed light on their functions.
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Enzymes of triacylglycerol synthesis and their regulation
TL;DR: De novo biosynthesis of triacylglycerol in eukaryotic cells, the isoenzymes that are involved, their subcellular locations, how they are regulated, and their putative individual roles in glycerolipid biosynthesis are focused on.
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Oils and Fats as Renewable Raw Materials in Chemistry
TL;DR: The remarkable advances made during the last decade in organic synthesis, catalysis, and biotechnology using plant oils and the basic oleochemicals derived from them will be reported, including, for example, ω-functionalization of fatty acids containing internal double bonds, application of the olefin metathesis reaction, and de novo synthesis of fatty fatty acids from abundantly available renewable carbon sources.
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Microdiesel: Escherichia coli engineered for fuel production.
TL;DR: Biodiesel-adequate FAEEs are established, referred to as Microdiesel, in metabolically engineered Escherichia coli by employing engineered micro-organisms, enabling a broader use of biodiesel-like fuels in the future.
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TL;DR: A new criterion for triggering the extension of word hits, combined with a new heuristic for generating gapped alignments, yields a gapped BLAST program that runs at approximately three times the speed of the original.
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Clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
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A simple method for displaying the hydropathic character of a protein
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TL;DR: A computer program that progressively evaluates the hydrophilicity and hydrophobicity of a protein along its amino acid sequence has been devised and its simplicity and its graphic nature make it a very useful tool for the evaluation of protein structures.
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Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence
Stewart T. Cole,Roland Brosch,Julian Parkhill,Thierry Garnier,Carol Churcher,David Harris,Stephen V. Gordon,Karin Eiglmeier,S. Gas,Clifton E. Barry,Fredj Tekaia,K. Badcock,D. Basham,D. Brown,Tracey Chillingworth,R. Connor,Robert L. Davies,K. Devlin,Theresa Feltwell,S. Gentles,N. Hamlin,S. Holroyd,T. Hornsby,Kay Jagels,Anders Krogh,J. McLean,Sharon Moule,Lee Murphy,K. Oliver,J. Osborne,Michael A. Quail,Marie-Adèle Rajandream,Jane Rogers,S. Rutter,K. Seeger,Jason Skelton,Rob Squares,S. Squares,John Sulston,K. Taylor,Sally Whitehead,Bart Barrell +41 more
TL;DR: The complete genome sequence of the best-characterized strain of Mycobacterium tuberculosis, H37Rv, has been determined and analysed in order to improve the understanding of the biology of this slow-growing pathogen and to help the conception of new prophylactic and therapeutic interventions.