Journal ArticleDOI
A peroxidase-coupled method for the colorimetric determination of serum triglycerides.
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TLDR
This sensitive chromogen system not only permits use of unusually small sample volumes, it also facilitates a linear response to serum triglyceride concentrations up to at least 10 g/L while displaying good Ringbom (measure of accuracy) characteristics.Abstract:
We describe an enzymatic method for rapid, precise measurement of serum triglycerides with use of sample:reagent ratios as large as 1:200. Hydrolysis of triglycerides is catalyzed by lipase to produce glycerol and free fatty acids. The glycerol generated is then phosphorylated by adenosine 5'-triphosphate in the presence of glycerol kinase. Oxidation of the resulting glycerol 3-phosphate to produce hydrogen peroxide is catalyzed by L-alpha-glycerophosphate oxidase. An intense red chromogen is produced by the peroxidase-catalyzed coupling of 4-aminoantipyrene and sodium 2-hydroxy-3,5-dichlorobenzenesulfonate with hydrogen peroxide. This sensitive chromogen system not only permits use of unusually small sample volumes, it also facilitates a linear response to serum triglyceride concentrations up to at least 10 g/L while displaying good Ringbom (measure of accuracy) characteristics.read more
Citations
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Journal ArticleDOI
Development of a normothermic extracorporeal liver perfusion system toward improving viability and function of human extended criteria donor livers.
Maria Franzini,Davide Ghinolfi,Daniele Pezzati,Franco Filipponi,Vanna Fierabracci,Aldo Paolicchi,Laura Caponi +6 more
TL;DR: Franzini et al. as discussed by the authors assessed coagulation factors and TG production during a procedure of extracorporeal normothermic perfusion that was conducted using a LiverAssist machine (Organ Assist, the Netherlands) on a liver procured from a 74year-old donor, that was eventually implanted in a male patient aged 53 years.
Journal ArticleDOI
Properties and stability of glycerophosphate oxidase isolated from a mutant strain of Aerococcus viridans.
TL;DR: The properties of microbial l‐α‐glycerophosphate oxidase isolated from a mutant strain of Aerococcus viridans DBM 1509 were estimated and the stability at different temperatures and pH were detected.
Journal ArticleDOI
Association between erythrocyte Na+K+-ATPase activity and some blood lipids in type 1 diabetic patients from Lagos, Nigeria.
TL;DR: It can be concluded that poor glycemic control evokes greater reduction in erythrocyte Na+K+-ATPase activity and promote enzyme-blood atherogenic lipid relationships in Type 1 diabetic Nigerian patients.
Journal ArticleDOI
Dietary fat and vitamin E have differential effects on serum lipid levels
TL;DR: The experiment was designed to study the effects of dietary fat and vitamin E on serum lipid status, an important indicator of risk for atherosclerosis, and found that serum vitamin E level was significantly greater in the corn oil group with 100 ppm vitamin E than in the lard group with 200 ppm.
Journal ArticleDOI
Antihyperglycemic and lipid lowering activities of ethanolic extract of eriobotrya japonica seeds in alloxan induced diabetic rats
Sabeeha Shafi,Nahida Tabassum +1 more
TL;DR: Ethanolic extract of EBJS is effective in controlling blood glucose levels and in improving lipid profile in diabetic rats and shows highly significant reduction in blood glucose and serum lipid profile levels in alloxan induced diabetic rats.
References
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Journal ArticleDOI
Quantitative Determination of Serum Triglycerides by the Use of Enzymes
Giovanni Bucolo,Harold David +1 more
TL;DR: A novel method for determining serum triglycerides, in which an enzymatic hydrolysis replaces the more commonly used saponification procedure, which is simple, rapid, and requires only 50 µl or less of sample.
Journal ArticleDOI
Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide.
P Fossati,L Prencipe +1 more
TL;DR: In this direct colorimetric procedure, serum triglycerides are hydrolyzed by lipase, and the released glycerol is assayed in a reaction catalyzed by glycersol kinase and L-alpha-glycerol-phosphate oxidase in a system that generates hydrogen peroxide.
Journal ArticleDOI
Micromethod for the direct determination of serum triglycerides.
E. Van Handel,D.B. Zilversmit +1 more
TL;DR: A microprocedure for the direct determination of triglyceride concentrations in biologic specimens is presented and depends on the quantitative removal of phosphatides from the sample and the subsequent determination of esterified glycerol.
Journal ArticleDOI
Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine.
TL;DR: A new direct colorimetric procedure for uric acid assay in serum or urine is described, utilizing a 3,5-dichloro-2-hydroxybenzene sulfonic acid/4-aminophenazone chromogenic system in the presence of horseradish peroxidase and uricase from Aspergillus flavus, affording useful results with sample/reagent volume ratios as low as 0.025.
Book
Instrumental methods of chemical analysis
TL;DR: In this article, the authors present a course to make the student understand the advanced instrumentation available for chemical analysis, and the student would be able to choose the instrument needed for analysis.