Journal ArticleDOI
A peroxidase-coupled method for the colorimetric determination of serum triglycerides.
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TLDR
This sensitive chromogen system not only permits use of unusually small sample volumes, it also facilitates a linear response to serum triglyceride concentrations up to at least 10 g/L while displaying good Ringbom (measure of accuracy) characteristics.Abstract:
We describe an enzymatic method for rapid, precise measurement of serum triglycerides with use of sample:reagent ratios as large as 1:200. Hydrolysis of triglycerides is catalyzed by lipase to produce glycerol and free fatty acids. The glycerol generated is then phosphorylated by adenosine 5'-triphosphate in the presence of glycerol kinase. Oxidation of the resulting glycerol 3-phosphate to produce hydrogen peroxide is catalyzed by L-alpha-glycerophosphate oxidase. An intense red chromogen is produced by the peroxidase-catalyzed coupling of 4-aminoantipyrene and sodium 2-hydroxy-3,5-dichlorobenzenesulfonate with hydrogen peroxide. This sensitive chromogen system not only permits use of unusually small sample volumes, it also facilitates a linear response to serum triglyceride concentrations up to at least 10 g/L while displaying good Ringbom (measure of accuracy) characteristics.read more
Citations
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Triglyceride measurements: a review of methods and interferences.
S G Klotzsch,J R McNamara +1 more
TL;DR: The major triglyceride quantification methods in the literature are reviewed, some of the potential interference problems, and the limitations regarding standardization that should be addressed when establishing such guidelines are addressed are reviewed.
Journal ArticleDOI
Alternative growth and energy storage responses to mortality threats in damselflies
TL;DR: Examination of effects of predation risk on relationships between growth and storage molecules in Enallagma aspersum and Ischnura verticalis damselfly larvae found species differences at the physiological level to the presence of mortality threats may be crucial to understanding patterns in metamorphic and post-metamorphic traits.
Journal ArticleDOI
The effect of dietary arachidonic acid on plasma lipoprotein distributions, apoproteins, blood lipid levels, and tissue fatty acid composition in humans.
G. J. Nelson,Perla C. Schmidt,G. Bartolini,Darshan S. Kelley,Stephen D. Phinney,David Kyle,Steven Silbermann,Ernst J. Schaefer +7 more
TL;DR: The results indicate that dietary AA is incorporated into tissue lipids, but selectively into different tissues and lipid classes, and demonstrates that dietaryAA does not alter blood lipids or lipoprotein levels or have obvious adverse health effects at this level and duration of feeding.
Journal ArticleDOI
Lipoprotein Lipase Binds to Low Density Lipoprotein Receptors and Induces Receptor-mediated Catabolism of Very Low Density Lipoproteins in Vitro
Jheem D. Medh,Susan L. Bowen,Glenna L. Fry,Stacie Ruben,Mark E. Andracki,Ituro Inoue,Jean-Marc Lalouel,Dudley K. Strickland,David A. Chappell +8 more
TL;DR: It is shown that LDL receptors also bind LPL and mediate LPL-dependent catabolism of large VLDL with Sf 100-400, and it is suggested that the carboxyl-terminal domain of LPL contributes to this interaction.
Journal ArticleDOI
Protein C and S deficiency, thrombophilia, and hypofibrinolysis: pathophysiologic causes of Legg-Perthes disease.
Charles J. Glueck,Helen I. Glueck,David Greenfield,Richard A. Freiberg,Alfred Kahn,T. Hamer,Davis Stroop,Trent Tracy +7 more
TL;DR: Of the eight patients with Legg-Perthes disease, four had thrombophilia and one had hypofibrinolysis, disorders that are believed to have contributed toThrombotic venous occlusion of the femur with subsequent venous hypertension and bone death that characterize Legg.
References
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Journal ArticleDOI
Quantitative Determination of Serum Triglycerides by the Use of Enzymes
Giovanni Bucolo,Harold David +1 more
TL;DR: A novel method for determining serum triglycerides, in which an enzymatic hydrolysis replaces the more commonly used saponification procedure, which is simple, rapid, and requires only 50 µl or less of sample.
Journal ArticleDOI
Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide.
P Fossati,L Prencipe +1 more
TL;DR: In this direct colorimetric procedure, serum triglycerides are hydrolyzed by lipase, and the released glycerol is assayed in a reaction catalyzed by glycersol kinase and L-alpha-glycerol-phosphate oxidase in a system that generates hydrogen peroxide.
Journal ArticleDOI
Micromethod for the direct determination of serum triglycerides.
E. Van Handel,D.B. Zilversmit +1 more
TL;DR: A microprocedure for the direct determination of triglyceride concentrations in biologic specimens is presented and depends on the quantitative removal of phosphatides from the sample and the subsequent determination of esterified glycerol.
Journal ArticleDOI
Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine.
TL;DR: A new direct colorimetric procedure for uric acid assay in serum or urine is described, utilizing a 3,5-dichloro-2-hydroxybenzene sulfonic acid/4-aminophenazone chromogenic system in the presence of horseradish peroxidase and uricase from Aspergillus flavus, affording useful results with sample/reagent volume ratios as low as 0.025.
Book
Instrumental methods of chemical analysis
TL;DR: In this article, the authors present a course to make the student understand the advanced instrumentation available for chemical analysis, and the student would be able to choose the instrument needed for analysis.