Journal ArticleDOI
A peroxidase-coupled method for the colorimetric determination of serum triglycerides.
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TLDR
This sensitive chromogen system not only permits use of unusually small sample volumes, it also facilitates a linear response to serum triglyceride concentrations up to at least 10 g/L while displaying good Ringbom (measure of accuracy) characteristics.Abstract:
We describe an enzymatic method for rapid, precise measurement of serum triglycerides with use of sample:reagent ratios as large as 1:200. Hydrolysis of triglycerides is catalyzed by lipase to produce glycerol and free fatty acids. The glycerol generated is then phosphorylated by adenosine 5'-triphosphate in the presence of glycerol kinase. Oxidation of the resulting glycerol 3-phosphate to produce hydrogen peroxide is catalyzed by L-alpha-glycerophosphate oxidase. An intense red chromogen is produced by the peroxidase-catalyzed coupling of 4-aminoantipyrene and sodium 2-hydroxy-3,5-dichlorobenzenesulfonate with hydrogen peroxide. This sensitive chromogen system not only permits use of unusually small sample volumes, it also facilitates a linear response to serum triglyceride concentrations up to at least 10 g/L while displaying good Ringbom (measure of accuracy) characteristics.read more
Citations
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Physiologic mechanisms for reduced apolipoprotein A-I concentrations associated with low levels of high density lipoprotein cholesterol in patients with normal plasma lipids.
TL;DR: For individuals with normal levels of plasma lipids, both residence times and input rates for apoA-I appeared to be important determinants of apo A-I levels, whereas input rates were highly variable among individuals.
Journal ArticleDOI
Causal Relationship between Adiponectin and Metabolic Traits: A Mendelian Randomization Study in a Multiethnic Population
Andrew Mente,Andrew Mente,David Meyre,David Meyre,Matthew B. Lanktree,Matthew B. Lanktree,Mahyar Heydarpour,A. Darlene Davis,Ruby Miller,Hertzel C. Gerstein,Hertzel C. Gerstein,Robert A. Hegele,Robert A. Hegele,Salim Yusuf,Salim Yusuf,Sonia S. Anand,Sonia S. Anand,Share-Ap Investigators +17 more
TL;DR: The association of the functional promoter polymorphism rs266729 with lower serum adiponectin and increased insulin resistance in diverse ethnic groups may suggest a causal relationship between adiponECTin level and insulin resistance.
Journal ArticleDOI
High-soluble-fiber Foods in Conjunction With a Telephone-Based, Personalized Behavior Change Support Service Result in Favorable Changes in Lipids and Lifestyles After 7 Weeks
Penny M. Kris-Etherton,Denise Shaffer Taylor,Helen Smiciklas-Wright,Diane C. Mitchell,Tanja Bekhuis,Beth H. Olson,Amy B Slonim +6 more
TL;DR: A 7-week intervention that includes both functional foods and individualized, interactive support for behavior change could be an effective model for dietitians to use with patients at risk for CVD, pending results of long-term studies.
Journal ArticleDOI
Evaluation of diagnostic reliability of DCA 2000 for rapid and simple monitoring of HbA1c.
M.P. Arsie,L. Marchioro,Annunziata Lapolla,G.F. Giacchetto,M.R. Bordin,P. Rizzotti,David A. Fedele +6 more
TL;DR: The DCA 2000 instrument (Bayer Diagnostics, Milan, Italy), based on an immunochemical technique, has been proposed for the rapid and simple evaluation of HbAlc, using even capillary blood as discussed by the authors.
Journal ArticleDOI
Alterations in gut microbiota composition and metabolic parameters after dietary intervention with barley beta glucans in patients with high risk for metabolic syndrome development.
TL;DR: The pre-intervention Gut microbiota composition showed higher abundance of health associated Bifidobacterium spp.
References
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Journal ArticleDOI
Quantitative Determination of Serum Triglycerides by the Use of Enzymes
Giovanni Bucolo,Harold David +1 more
TL;DR: A novel method for determining serum triglycerides, in which an enzymatic hydrolysis replaces the more commonly used saponification procedure, which is simple, rapid, and requires only 50 µl or less of sample.
Journal ArticleDOI
Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide.
P Fossati,L Prencipe +1 more
TL;DR: In this direct colorimetric procedure, serum triglycerides are hydrolyzed by lipase, and the released glycerol is assayed in a reaction catalyzed by glycersol kinase and L-alpha-glycerol-phosphate oxidase in a system that generates hydrogen peroxide.
Journal ArticleDOI
Micromethod for the direct determination of serum triglycerides.
E. Van Handel,D.B. Zilversmit +1 more
TL;DR: A microprocedure for the direct determination of triglyceride concentrations in biologic specimens is presented and depends on the quantitative removal of phosphatides from the sample and the subsequent determination of esterified glycerol.
Journal ArticleDOI
Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine.
TL;DR: A new direct colorimetric procedure for uric acid assay in serum or urine is described, utilizing a 3,5-dichloro-2-hydroxybenzene sulfonic acid/4-aminophenazone chromogenic system in the presence of horseradish peroxidase and uricase from Aspergillus flavus, affording useful results with sample/reagent volume ratios as low as 0.025.
Book
Instrumental methods of chemical analysis
TL;DR: In this article, the authors present a course to make the student understand the advanced instrumentation available for chemical analysis, and the student would be able to choose the instrument needed for analysis.