Journal ArticleDOI
A peroxidase-coupled method for the colorimetric determination of serum triglycerides.
Reads0
Chats0
TLDR
This sensitive chromogen system not only permits use of unusually small sample volumes, it also facilitates a linear response to serum triglyceride concentrations up to at least 10 g/L while displaying good Ringbom (measure of accuracy) characteristics.Abstract:
We describe an enzymatic method for rapid, precise measurement of serum triglycerides with use of sample:reagent ratios as large as 1:200. Hydrolysis of triglycerides is catalyzed by lipase to produce glycerol and free fatty acids. The glycerol generated is then phosphorylated by adenosine 5'-triphosphate in the presence of glycerol kinase. Oxidation of the resulting glycerol 3-phosphate to produce hydrogen peroxide is catalyzed by L-alpha-glycerophosphate oxidase. An intense red chromogen is produced by the peroxidase-catalyzed coupling of 4-aminoantipyrene and sodium 2-hydroxy-3,5-dichlorobenzenesulfonate with hydrogen peroxide. This sensitive chromogen system not only permits use of unusually small sample volumes, it also facilitates a linear response to serum triglyceride concentrations up to at least 10 g/L while displaying good Ringbom (measure of accuracy) characteristics.read more
Citations
More filters
Journal ArticleDOI
Dietary intake and plasma concentrations of vitamin E, vitamin C, and beta carotene in patients with coronary artery disease.
Journal ArticleDOI
Plasma lipid effects of corn oil and extra-virgin olive oil in hypercholesterolaemic subjects: a randomised, controlled trial
TL;DR: In this paper, the metabolic effect of monounsaturated fatty acid rich oils and polyunsaturated fatty acids rich oils on the plasma lipid pattern of moderately hypercholesterolaemic subjects under controlled isocaloric condition was directly compared.
Correlation of Serum Lipids and Lipoproteins with Trace Elements in Water Buffalo (Bubalus bubalis)
Javad Tajik,Saeed Nazifi +1 more
TL;DR: In this article, the correlations of the serum concentrations of trace elements with lipids and lipoproteins in water buffalo (Bubalus bubalis) were measured in 100 clinically healthy water buffaloes.
Journal ArticleDOI
Arbutin prevents alterations in mitochondrial and lysosomal enzymes in isoproterenol-induced myocardial infarction: An in vivo study.
TL;DR: It is demonstrated that the 50 mg/kg bw of ARB shows higher protection against ISO-induced damage and on the DNA damage in rats with isoproterenol (ISO)-induced myocardial infarction.
Journal ArticleDOI
Hypolipidaemic Effect of Hericium erinaceum Grown in Artemisia capillaris on Obese Rats.
TL;DR: In this paper, the authors used ethanolic extracts from Hericium erinaceum (HEAC) and Artemisia capillaris (CAPillaris) to lower the cholesterol levels of male Sprague-Dawley rats fed a high-fat diet.
References
More filters
Journal ArticleDOI
Quantitative Determination of Serum Triglycerides by the Use of Enzymes
Giovanni Bucolo,Harold David +1 more
TL;DR: A novel method for determining serum triglycerides, in which an enzymatic hydrolysis replaces the more commonly used saponification procedure, which is simple, rapid, and requires only 50 µl or less of sample.
Journal ArticleDOI
Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide.
P Fossati,L Prencipe +1 more
TL;DR: In this direct colorimetric procedure, serum triglycerides are hydrolyzed by lipase, and the released glycerol is assayed in a reaction catalyzed by glycersol kinase and L-alpha-glycerol-phosphate oxidase in a system that generates hydrogen peroxide.
Journal ArticleDOI
Micromethod for the direct determination of serum triglycerides.
E. Van Handel,D.B. Zilversmit +1 more
TL;DR: A microprocedure for the direct determination of triglyceride concentrations in biologic specimens is presented and depends on the quantitative removal of phosphatides from the sample and the subsequent determination of esterified glycerol.
Journal ArticleDOI
Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine.
TL;DR: A new direct colorimetric procedure for uric acid assay in serum or urine is described, utilizing a 3,5-dichloro-2-hydroxybenzene sulfonic acid/4-aminophenazone chromogenic system in the presence of horseradish peroxidase and uricase from Aspergillus flavus, affording useful results with sample/reagent volume ratios as low as 0.025.
Book
Instrumental methods of chemical analysis
TL;DR: In this article, the authors present a course to make the student understand the advanced instrumentation available for chemical analysis, and the student would be able to choose the instrument needed for analysis.