Journal ArticleDOI
A peroxidase-coupled method for the colorimetric determination of serum triglycerides.
Reads0
Chats0
TLDR
This sensitive chromogen system not only permits use of unusually small sample volumes, it also facilitates a linear response to serum triglyceride concentrations up to at least 10 g/L while displaying good Ringbom (measure of accuracy) characteristics.Abstract:
We describe an enzymatic method for rapid, precise measurement of serum triglycerides with use of sample:reagent ratios as large as 1:200. Hydrolysis of triglycerides is catalyzed by lipase to produce glycerol and free fatty acids. The glycerol generated is then phosphorylated by adenosine 5'-triphosphate in the presence of glycerol kinase. Oxidation of the resulting glycerol 3-phosphate to produce hydrogen peroxide is catalyzed by L-alpha-glycerophosphate oxidase. An intense red chromogen is produced by the peroxidase-catalyzed coupling of 4-aminoantipyrene and sodium 2-hydroxy-3,5-dichlorobenzenesulfonate with hydrogen peroxide. This sensitive chromogen system not only permits use of unusually small sample volumes, it also facilitates a linear response to serum triglyceride concentrations up to at least 10 g/L while displaying good Ringbom (measure of accuracy) characteristics.read more
Citations
More filters
Journal ArticleDOI
Cholesterol ester transfer protein, apolipoprotein E and lipoprotein lipase genotypes in patients with coronary artery disease in the Turkish population.
TL;DR: It is demonstrated that the CETP Msp1 and Apo E gene polymorphisms are associated with variations in lipids in patients with CAD and healthy controls in Turkish population.
Journal Article
Effect of conventional antihypertensive drugs on hypolipidemic action of garlic in rats.
TL;DR: It was concluded that administration of PRO and HYD decrease the hypolipidemic effect of GH and administration of GH along with CAP augmented the hypoledepidemicEffect of GH in rats.
Journal ArticleDOI
Prevalence of Metabolic Syndrome in Psoriasis Patients and its Relation to Disease Duration: A Hospital Based Case-Control Study.
TL;DR: The findings suggest that metabolic syndrome as well as dyslipidaemia is commoner in psoriasis patients, and underlines the need for screening of all psoriiasis patients for early diagnosis and treatment of associated metabolic syndrome to reduce the high burden of morbidity and mortality.
Journal ArticleDOI
Antihyperlipidemic effect of carvacrol on D-galactosamine-induced hepatotoxic rats.
Balakrishnan Aristatile,Khalid S. Al-Numair,Chinnadurai Veeramani,Kodukkur Viswanathan Pugalendi +3 more
TL;DR: The results suggest that carvacrol affords a significant hepatoprotective and hypolipidemic effect against D-GalN-induced-rats.
Journal ArticleDOI
An experimental evaluation of the anti-atherogenic potential of the plant, Piper betle, and its active constitutent, eugenol, in rats fed an atherogenic diet
TL;DR: Results strongly suggest that the Piper betle extract and its active constituent, eugenol, exhibit anti-atherogenic effects which may be due to their anti-oxidative properties.
References
More filters
Journal ArticleDOI
Quantitative Determination of Serum Triglycerides by the Use of Enzymes
Giovanni Bucolo,Harold David +1 more
TL;DR: A novel method for determining serum triglycerides, in which an enzymatic hydrolysis replaces the more commonly used saponification procedure, which is simple, rapid, and requires only 50 µl or less of sample.
Journal ArticleDOI
Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide.
P Fossati,L Prencipe +1 more
TL;DR: In this direct colorimetric procedure, serum triglycerides are hydrolyzed by lipase, and the released glycerol is assayed in a reaction catalyzed by glycersol kinase and L-alpha-glycerol-phosphate oxidase in a system that generates hydrogen peroxide.
Journal ArticleDOI
Micromethod for the direct determination of serum triglycerides.
E. Van Handel,D.B. Zilversmit +1 more
TL;DR: A microprocedure for the direct determination of triglyceride concentrations in biologic specimens is presented and depends on the quantitative removal of phosphatides from the sample and the subsequent determination of esterified glycerol.
Journal ArticleDOI
Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine.
TL;DR: A new direct colorimetric procedure for uric acid assay in serum or urine is described, utilizing a 3,5-dichloro-2-hydroxybenzene sulfonic acid/4-aminophenazone chromogenic system in the presence of horseradish peroxidase and uricase from Aspergillus flavus, affording useful results with sample/reagent volume ratios as low as 0.025.
Book
Instrumental methods of chemical analysis
TL;DR: In this article, the authors present a course to make the student understand the advanced instrumentation available for chemical analysis, and the student would be able to choose the instrument needed for analysis.