Journal ArticleDOI
A peroxidase-coupled method for the colorimetric determination of serum triglycerides.
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TLDR
This sensitive chromogen system not only permits use of unusually small sample volumes, it also facilitates a linear response to serum triglyceride concentrations up to at least 10 g/L while displaying good Ringbom (measure of accuracy) characteristics.Abstract:
We describe an enzymatic method for rapid, precise measurement of serum triglycerides with use of sample:reagent ratios as large as 1:200. Hydrolysis of triglycerides is catalyzed by lipase to produce glycerol and free fatty acids. The glycerol generated is then phosphorylated by adenosine 5'-triphosphate in the presence of glycerol kinase. Oxidation of the resulting glycerol 3-phosphate to produce hydrogen peroxide is catalyzed by L-alpha-glycerophosphate oxidase. An intense red chromogen is produced by the peroxidase-catalyzed coupling of 4-aminoantipyrene and sodium 2-hydroxy-3,5-dichlorobenzenesulfonate with hydrogen peroxide. This sensitive chromogen system not only permits use of unusually small sample volumes, it also facilitates a linear response to serum triglyceride concentrations up to at least 10 g/L while displaying good Ringbom (measure of accuracy) characteristics.read more
Citations
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Use of a nonionic detergent (Triton WR 1339) in healthy cats to assess hepatic secretion of triglyceride.
TL;DR: Triton WR 1339 can be administered IV to cats at a rate of 150 mg/kg to assess hepatic triglyceride secretion, although some cats may have increased RBC osmotic fragility.
Journal ArticleDOI
ApoE isoforms, treatment of diabetes and the risk of coronary heart disease.
Hideki Ehara,Ritsuko Yamamoto-Honda,Hiroji Kitazato,Yoshihiko Takahashi,Shoji Kawazu,Yasuo Akanuma,Mitsuhiko Noda +6 more
TL;DR: Under standard medical treatment of diabetes, including the control of LDL cholesterol levels, the apoE4 isoform was not associated with coronary heart disease among T2DM patients.
Journal ArticleDOI
Seasonal changes in morphometric and biochemical endpoints in northern pike (Esox lucius), burbot (Lota lota) and slimy sculpin (Cottus cognatus)
TL;DR: Biochemical estimates of fish growth exhibited clear seasonal differences, with consistent decreases in this endpoint for all fish species in spring relative to the previous autumn at both exposure and reference sites, however, results for total body lipids and triglycerides were inconclusive and inconsistent between species and exposure scenarios.
Journal ArticleDOI
Studies on the Development of a Non-genetic Obese Type 2 Diabetic Rat Model: Amelioration by (+)-Catechin Hydrate
TL;DR: High fat and high sucrose diet is useful in the development of an obese type 2 diabetic rat model and catechin to some extent is beneficial.
Journal ArticleDOI
Clinical Chemistry through Clinical Chemistry: A Journal Timeline
TL;DR: Specific areas of laboratory medicine particularly well represented in Clinical Chemistry include lipids, endocrinology, protein markers, quality of laboratory measurements, molecular diagnostics, and general advances in methodology and instrumentation.
References
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Journal ArticleDOI
Quantitative Determination of Serum Triglycerides by the Use of Enzymes
Giovanni Bucolo,Harold David +1 more
TL;DR: A novel method for determining serum triglycerides, in which an enzymatic hydrolysis replaces the more commonly used saponification procedure, which is simple, rapid, and requires only 50 µl or less of sample.
Journal ArticleDOI
Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide.
P Fossati,L Prencipe +1 more
TL;DR: In this direct colorimetric procedure, serum triglycerides are hydrolyzed by lipase, and the released glycerol is assayed in a reaction catalyzed by glycersol kinase and L-alpha-glycerol-phosphate oxidase in a system that generates hydrogen peroxide.
Journal ArticleDOI
Micromethod for the direct determination of serum triglycerides.
E. Van Handel,D.B. Zilversmit +1 more
TL;DR: A microprocedure for the direct determination of triglyceride concentrations in biologic specimens is presented and depends on the quantitative removal of phosphatides from the sample and the subsequent determination of esterified glycerol.
Journal ArticleDOI
Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine.
TL;DR: A new direct colorimetric procedure for uric acid assay in serum or urine is described, utilizing a 3,5-dichloro-2-hydroxybenzene sulfonic acid/4-aminophenazone chromogenic system in the presence of horseradish peroxidase and uricase from Aspergillus flavus, affording useful results with sample/reagent volume ratios as low as 0.025.
Book
Instrumental methods of chemical analysis
TL;DR: In this article, the authors present a course to make the student understand the advanced instrumentation available for chemical analysis, and the student would be able to choose the instrument needed for analysis.