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Journal ArticleDOI

A probable lipid transfer protein gene is induced by NaCl in stems of tomato plants.

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TLDR
The high similarity between the T SW12-deduced amino acid sequence and reported lipid transfer proteins suggests that TSW12 encodes a lipid transfer protein.
Abstract
A full-length tomato cDNA clone, TSW12, which is developmentally and environmentally regulated, has been isolated and characterized. TSW12 mRNA is accumulated during tomato seed germination and its level increases after NaCl treatment or heat shock. In mature plants, TSW12 mRNA is only detected upon treatment with NaCl, mannitol or ABA and its expression mainly occurs in stems. The nucleotide sequence of TSW12 includes an open reading frame coding for a basic protein of 114 amino acids; the first 23 amino acids exhibit the sequence characteristic of a signal peptide. The high similarity between the TSW12-deduced amino acid sequence and reported lipid transfer proteins suggests that TSW12 encodes a lipid transfer protein.

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Journal ArticleDOI

Salt tolerance and salinity effects on plants: a review.

TL;DR: The ability of plants to tolerate salt is determined by multiple biochemical pathways that facilitate retention and/or acquisition of water, protect chloroplast functions, and maintain ion homeostasis as mentioned in this paper.
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The molecular basis of dehydration tolerance in plants

TL;DR: A large number of genes with a potential role in drought tolerance have been described, and major themes in the molecular response have been established.
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Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray.

TL;DR: A full-length cDNA microarray containing approximately 7000 independent, full- length cDNA groups is prepared to analyse the expression profiles of genes under drought, cold (low temperature) and high-salinity stress conditions over time, suggesting that various transcriptional regulatory mechanisms function in the drought,cold or high- salinity stress signal transduction pathways.
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The effect of drought and heat stress on reproductive processes in cereals.

TL;DR: The results achieved so far indicate that various plant organs, in a definite hierarchy and in interaction with each other, are involved in determining crop yield under stress.
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Monitoring Expression Profiles of Rice Genes under Cold, Drought, and High-Salinity Stresses and Abscisic Acid Application Using cDNA Microarray and RNA Gel-Blot Analyses

TL;DR: In this article, a rice cDNA microarray was used to identify cold-, drought-, high-salinity-, and/or abscisic acid (ABA)-inducible genes in rice (Oryza sativa).
References
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Book

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TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI

DNA sequencing with chain-terminating inhibitors

TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
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Rapid isolation of high molecular weight plant DNA

TL;DR: A method is presented for the rapid isolation of high molecular weight plant DNA which is free of contaminants which interfere with complete digestion by restriction endonucleases, and which yields total cellular DNA.
Journal ArticleDOI

Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.

TL;DR: The algorithm is shown to be at least as good as, and usually superior to, the reported prediction methods assessed in the same way and the implication in protein folding is discussed.
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