Journal ArticleDOI
A sensitive SDS-PAGE method separating myosin heavy chain isoforms of rat skeletal muscles reveals the heterogeneous nature of the embryonic myosin.
Ugo Carraro,Claudia Catani +1 more
TLDR
A sensitive method is described that separates in nanogram or microgram amounts the heavy chains of immature, fast and slow adult rat muscles in complex mixtures of myosins and reveals the heterogeneous nature of the embryonic myosin heavy chains.About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 1983-11-15. It has received 193 citations till now. The article focuses on the topics: Myosin.read more
Citations
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Journal ArticleDOI
Electrophoretic separation of rat skeletal muscle myosin heavy-chain isoforms
Robert J. Talmadge,Roland R. Roy +1 more
TL;DR: The percent composition of the four adult MHCs in rat soleus, medial gastrocnemius, diaphragm, and levator ani muscles by use of this procedure and Coomasie Blue staining is similar to that previously reported.
Book ChapterDOI
Cellular and molecular diversities of mammalian skeletal muscle fibers
Dirk Pette,Robert S. Staron +1 more
Journal ArticleDOI
Function, morphology and protein expression of ageing skeletal muscle: a cross-sectional study of elderly men with different training backgrounds.
H. Klitgaard,M Mantoni,Stefano Schiaffino,Simonetta Ausoni,Luisa Gorza,C Laurent-Winter,P Schnohr,Bengt Saltin +7 more
TL;DR: The results seem to suggest that strength training can counteract the age-related changes in function and morphology of the ageing human skeletal muscle.
Journal ArticleDOI
Maximum velocity of shortening in relation to myosin isoform composition in single fibres from human skeletal muscles.
Lars Larsson,Richard L. Moss +1 more
TL;DR: Maximum tension (P0) and specific tension were lower in freeze‐dried types I and IIA fibres than in chemically skinned, while no differences were observed in the IIA/B fibres.
Journal ArticleDOI
Shortening velocity in single fibers from adult rabbit soleus muscles is correlated with myosin heavy chain composition.
TL;DR: A specific contractile property, velocity of shortening, and protein subunit composition of single fibers from adult rabbit soleus muscles are characterized, suggesting that some myosin molecules consist of mixtures of slow- and fast-type subunits.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
TL;DR: A rapid and convenient method for peptide mapping of proteins has been developed that involves partial enzymatic proteolysis in the presence of sodium dodecyl sulfate and analysis of the cleavage products by polyacrylamide gel electrophoresis.
Journal ArticleDOI
Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins
TL;DR: A new silver stain for electrophoretically separated polypeptides can be rapidly and easily used and can detect as little as 0.01 nanogram of protein per square millimeter when employed with two-dimensional electrophoresis.
Journal ArticleDOI
ATPase Activity of Myosin Correlated with Speed of Muscle Shortening
TL;DR: A role for the ATPase activity of myosin in determining the speed of muscle contraction is suggested and the F-actin-binding ability of myOSin from various muscles was rather constant.