A Virus with a Bisegmented Double-stranded RNA Genome in Rat (Oryzomys nigripes) Intestines
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TLDR
Examination of the intestinal contents of free-living Oryzomys nigripes rats by PAGE revealed two sharply defined bands that could be stained by ethidium bromide or by silver nitrate with comparable intensities, suggesting that these particles represent an as yet undescribed virus with a bisegmented double-stranded RNA genome, for which the name 'picobirnavirus' is proposed.Abstract:
Examination of the intestinal contents of free-living Oryzomys nigripes rats by PAGE revealed two sharply defined bands that could be stained by ethidium bromide or by silver nitrate with comparable intensities. The molecules forming these bands were susceptible to digestion by pancreatic RNase A but not by RNase T1 or by DNase I. Their lengths were estimated to be about 2.6 and 1.5 kbp, respectively, by comparison with rotavirus SA11 genome segments. They cosedimented in CsCl gradients at a density of 1.39 to 1.40 g/ml, together with uniform particles approximately 35 nm in diameter with indistinct surface structure. It is suggested that these particles represent an as yet undescribed virus with a bisegmented double-stranded RNA genome, for which the name 'picobirnavirus' is proposed.read more
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Divergent Picobirnaviruses in Human Feces
Terry Fei Fan Ng,Terry Fei Fan Ng,Everardo Vega,Nikola O. Kondov,Christopher Markey,Xutao Deng,Xutao Deng,Nicole Gregoricus,Jan Vinjé,Eric Delwart,Eric Delwart +10 more
TL;DR: The near-complete genomes of two picobirnaviruses (PBVs) in diarrheal stool samples, human picobiraNaviruses D and E (HuPBV-D and -E), were genetically characterized and their RNA-dependent RNA polymerase (RdRp) protein sequences had <66% identities to known PBVs.
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Biophysical and biochemical properties of an unusual birnavirus pathogenic for rotifers.
TL;DR: All the other biochemical and biophysical characters indicate that RBV is a member of the Birnaviridae family with, for the moment, a unique position in this group.
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Detection and Molecular Characterization of Porcine Picobirnavirus in Feces of Domestic Pigs from Kolkata, India
Balasubramanian Ganesh,Krisztián Bányai,Suman Kanungo,Deepika Sur,Yashpal Singh Malik,Nobumichi Kobayashi +5 more
TL;DR: In this article, the authors reported the first detection and molecular characterization of porcine Picobirnaviruses (PBV) in domestic pigs from India using the human PBV genogroup I specific primer pair: PicoB25(+) and picoB43(−).
Journal ArticleDOI
Molecular epidemiology and characterization of picobirnaviruses in small ruminant populations in India.
Yashpal Singh Malik,Shubhankar Sircar,Kuldeep Dhama,Rashmi Singh,Souvik Ghosh,Krisztián Bányai,Anastasia N. Vlasova,Touil Nadia,Raj Kumar Singh +8 more
TL;DR: In this article, the authors reported that Picobirnavirus (PBV) infection in small ruminants (ovine and caprine), in India was reported in 2015 to 2017.
Journal ArticleDOI
Maintenance of picobirnavirus (PBV) infection in an adult orangutan (Pongo pygmaeus) and genetic diversity of excreted viral strains during a three-year period.
Gisela Masachessi,Gisela Masachessi,Balasubramanian Ganesh,Laura C. Martinez,Miguel O. Giordano,Patricia A. Barril,Patricia A. Barril,María Beatriz Isa,Giorgio V. Paván,Carlos A. Mateos,Silvia Nates +10 more
TL;DR: The results obtained allowed to arrive to a deeper understanding of the natural history of PBV infection, which seems to be characterized by new-born, juvenile and adult asymptomatic hosts which persistently excrete closely related strains in their feces.
References
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TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
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Journal ArticleDOI
Biophysical and biochemical characterization of five animal viruses with bisegmented double-stranded RNA genomes.
TL;DR: Infectious pancreatic necrosis virus of fish, infectious bursal disease virus of chickens, Tellina virus and oyster virus of bivalve molluscs, and drosophila X virus of Drosophila melanogaster are naked icosahedral viruses with an electron microscopic diameter of 58 to 60 nm as mentioned in this paper.
Biophysical andBiochemical Characterization ofFiveAnimal Viruses withBisegmented Double-Stranded RNA Genomes
TL;DR: Tryptic peptide comparisons of 125I-labeled virion proteins showed that five viruses are different from each other, although there was considerable overlap in the peptide maps of the three aquatic viruses, indicting a degree of relatedness.