A Virus with a Bisegmented Double-stranded RNA Genome in Rat (Oryzomys nigripes) Intestines
TLDR
Examination of the intestinal contents of free-living Oryzomys nigripes rats by PAGE revealed two sharply defined bands that could be stained by ethidium bromide or by silver nitrate with comparable intensities, suggesting that these particles represent an as yet undescribed virus with a bisegmented double-stranded RNA genome, for which the name 'picobirnavirus' is proposed.Abstract:
Examination of the intestinal contents of free-living Oryzomys nigripes rats by PAGE revealed two sharply defined bands that could be stained by ethidium bromide or by silver nitrate with comparable intensities. The molecules forming these bands were susceptible to digestion by pancreatic RNase A but not by RNase T1 or by DNase I. Their lengths were estimated to be about 2.6 and 1.5 kbp, respectively, by comparison with rotavirus SA11 genome segments. They cosedimented in CsCl gradients at a density of 1.39 to 1.40 g/ml, together with uniform particles approximately 35 nm in diameter with indistinct surface structure. It is suggested that these particles represent an as yet undescribed virus with a bisegmented double-stranded RNA genome, for which the name 'picobirnavirus' is proposed.read more
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Detection of Genogroup I and II human picobirnaviruses showing small genomic RNA profile causing acute watery diarrhoea among children in Kolkata, India.
R. Bhattacharya,Ganesh Chandra Sahoo,Mukti Kant Nayak,Krishnan Rajendran,P. Dutta,Utpala Mitra,M. K. Bhattacharya,T. N. Naik,S. K. Bhattacharya,Triveni Krishnan +9 more
TL;DR: The short genome profile PBVs associated with acute watery diarrhoea may be another emerging diarrhoeagenic virus in Kolkata, India, where four PBV positives of Genogroup II were detected during this study.
Journal ArticleDOI
Identification in porcine faeces of a novel virus with a bisegmented double stranded RNA genome.
TL;DR: The results confirm the circulation among pigs of a novel virus, possibly of vertebrates, with a bisegmented double stranded RNA genome, similar to viruses previously described in humans, wild rats, guinea pigs, pigs, and chickens, for which the name “picobirnavirus” has been proposed.
Journal ArticleDOI
Picobirnavirus infections: viral persistence and zoonotic potential
Balasubramanian Ganesh,Krisztián Bányai,Vito Martella,Ferenc Jakab,Gisela Masachessi,Nobumichi Kobayashi +5 more
TL;DR: The public health aspects of PBV infection, especially its possible association with zoonosis is analyzed, as well as evidence has been found for genetic relatedness between human and animalPBV strains, suggesting extant crossing points in the ecology and evolution of heterologous PBV strains.
Short Communication Genogroup I picobirnaviruses in pigs: evidence for genetic diversity and relatedness to human strains
TL;DR: The molecular analysis of porcine PBVs identified in the intestinal content of dead pigs suggested that PBVs exist as quasispecies in the swine alimentary tract and indicated that infection with genogroup I PBVs is common in pigs.
Journal ArticleDOI
Viruses with bisegmented double-stranded RNA in pig faeces.
Maria Silvia Viccari Gatti,A. F. Pestana de Castro,M. M. G. Ferraz,A.M. Fialfio,H.G. Pereira +4 more
TL;DR: Viruses similar to the bisegmented double-stranded (ds) RNA picobirnaviruses described in human faeces and the intestinal contents of Oryzomys nigripes rats and guinea pigs were isolated from the Faeces of pigs taken from several areas in the state of Sao Paulo, Brazil.
References
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Journal Article
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Journal ArticleDOI
Biophysical and biochemical characterization of five animal viruses with bisegmented double-stranded RNA genomes.
TL;DR: Infectious pancreatic necrosis virus of fish, infectious bursal disease virus of chickens, Tellina virus and oyster virus of bivalve molluscs, and drosophila X virus of Drosophila melanogaster are naked icosahedral viruses with an electron microscopic diameter of 58 to 60 nm as mentioned in this paper.
Biophysical andBiochemical Characterization ofFiveAnimal Viruses withBisegmented Double-Stranded RNA Genomes
TL;DR: Tryptic peptide comparisons of 125I-labeled virion proteins showed that five viruses are different from each other, although there was considerable overlap in the peptide maps of the three aquatic viruses, indicting a degree of relatedness.