ABI1 and PP2CA Phosphatases Are Negative Regulators of Snf1-Related Protein Kinase1 Signaling in Arabidopsis
Americo Rodrigues,Americo Rodrigues,Mattia Adamo,Pierre Crozet,Leonor Margalha,Ana Confraria,Claudia Martinho,Alexandre Elias,Agnese Rabissi,Victoria Lumbreras,Miguel González-Guzmán,Regina Antoni,Pedro L. Rodriguez,Elena Baena-González +13 more
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TLDR
It is demonstrated that two clade A type 2C protein phosphatases, established repressors of the abscisic acid hormonal pathway, interact with the SnRK1 catalytic subunit causing its dephosphorylation and inactivation, strengthening the stress response through the cooperation of two key and complementary pathways.Abstract:
Plant survival under environmental stress requires the integration of multiple signaling pathways into a coordinated response, but the molecular mechanisms underlying this integration are poorly understood. Stress-derived energy deprivation activates the Snf1-related protein kinases1 (SnRK1s), triggering a vast transcriptional and metabolic reprogramming that restores homeostasis and promotes tolerance to adverse conditions. Here, we show that two clade A type 2C protein phosphatases (PP2Cs), established repressors of the abscisic acid (ABA) hormonal pathway, interact with the SnRK1 catalytic subunit causing its dephosphorylation and inactivation. Accordingly, SnRK1 repression is abrogated in double and quadruple pp2c knockout mutants, provoking, similarly to SnRK1 overexpression, sugar hypersensitivity during early seedling development. Reporter gene assays and SnRK1 target gene expression analyses further demonstrate that PP2C inhibition by ABA results in SnRK1 activation, promoting SnRK1 signaling during stress and once the energy deficit subsides. Consistent with this, SnRK1 and ABA induce largely overlapping transcriptional responses. Hence, the PP2C hub allows the coordinated activation of ABA and energy signaling, strengthening the stress response through the cooperation of two key and complementary pathways.read more
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Sugar signals and the control of plant growth and development
TL;DR: This review focuses on information presented in the past 2 years on key players in sugar-mediated plant growth regulation, with emphasis on trehalose 6-phosphate, target of rapamycin kinase, and Snf1-related kinase 1 regulatory systems.
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Alternative Splicing Control of Abiotic Stress Responses.
TL;DR: Current understanding of post-transcriptional control of plant stress tolerance via alternative splicing viaAlternative splicing is reviewed and research challenges for the near future are discussed.
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The role of ubiquitin and the 26S proteasome in plant abiotic stress signaling.
TL;DR: Role of the UPS in modulating protein stability during abiotic stress signaling is provided and E3 ubiquitin ligases for which stress-related substrate proteins have been identified are discussed.
Journal ArticleDOI
Degradation of the ABA co-receptor ABI1 by PUB12/13 U-box E3 ligases
Lingyao Kong,Jinkui Cheng,Yujuan Zhu,Yanglin Ding,Jingjing Meng,Zhizhong Chen,Qi Xie,Yan Guo,Jigang Li,Shuhua Yang,Zhizhong Gong +10 more
TL;DR: It is reported that ABI1 (ABA-INSENSITIVE 1) can interact with the U-box E3 ligases PUB12 and PUB13, but is ubiquitinated only when it interacts with ABA receptors in an in vitro assay.
Journal ArticleDOI
Source-Sink Communication: Regulated by Hormone, Nutrient, and Stress Cross-Signaling.
TL;DR: Recent advances in understanding of the molecular mechanisms regulating sugar mobilization during seed development and seedling establishment in cereals are discussed, which provide the majority of nutrition for humans.
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Steven K. Hanks,Tony Hunter +1 more
TL;DR: The eukaryotic protein kinases make up a large superfamily of homologous proteins, and a classification scheme can be founded on a kinase domain phylogeny, which reveals families of enzymes that have related substrate specificities and modes of regulation.
Journal ArticleDOI
Abscisic Acid: Emergence of a Core Signaling Network
TL;DR: A new model for ABA action has been proposed and validated, in which the soluble PYR/PYL/RCAR receptors function at the apex of a negative regulatory pathway to directly regulate PP2C phosphatases, which in turn directly regulate SnRK2 kinases.
Journal ArticleDOI
Abscisic Acid Inhibits Type 2C Protein Phosphatases via the PYR/PYL Family of START Proteins
Sang-Youl Park,Pauline Fung,Noriyuki Nishimura,Davin R. Jensen,Hiroaki Fujii,Yang Zhao,Shelley Lumba,Julia Santiago,Americo Rodrigues,Tsz-fung Freeman Chow,Simon E. Alfred,Dario Bonetta,Ruth R. Finkelstein,Nicholas J. Provart,Darrell Desveaux,Pedro L. Rodriguez,Peter McCourt,Jian-Kang Zhu,Julian I. Schroeder,Brian F. Volkman,Sean R. Cutler +20 more
TL;DR: PYR/PYLs are ABA receptors functioning at the apex of a negative regulatory pathway that controls ABA signaling by inhibiting PP2Cs, illustrating the power of the chemical genetic approach for sidestepping genetic redundancy.
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Abscisic Acid Inhibits Type 2C Protein Phosphatases via the PYR/PYL Family of START Proteins
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