Journal ArticleDOI
Abundant alkali-sensitive sites in DNA of human and mouse sperm☆
Narendra P. Singh,David B. Danner,Raymond R. Tice,Michael T. McCoy,Gary D. Collins,Edward L. Schneider +5 more
TLDR
The high frequency of single-strand DNA breaks in sperm from healthy mouse and human donors suggests that they represent a functional characteristic of condensed chromatin rather than DNA damage.About:
This article is published in Experimental Cell Research.The article was published on 1989-10-01. It has received 257 citations till now. The article focuses on the topics: DNA damage & Agarose gel electrophoresis.read more
Citations
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Journal ArticleDOI
The comet assay: a comprehensive review
TL;DR: The principles of strand break detection using both the alkaline and neutral versions of the technique are discussed, and a basic methodology with currently used variations is presented.
Journal ArticleDOI
Sperm chromatin structure assay: its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques.
Journal ArticleDOI
The single cell gel electrophoresis assay (comet assay): A European review
Valerie J. McKelvey-Martin,Michael H.L. Green,P. Schmezer,Beatrice L. Pool-Zobel,M.P. De Méo,Andrew Collins +5 more
TL;DR: The development of the SCGE assay, existing protocols for the detection and analysis of comets, the relevant underlying principles determining the behaviour of DNA, and the potential applications of the technique are reviewed.
Journal ArticleDOI
Relative Impact of Oxidative Stress on the Functional Competence and Genomic Integrity of Human Spermatozoa
TL;DR: Human spermatozoa exposed to increasing levels of oxidative stress achieved through the stimulation of endogenous oxidant generation with NADPH or direct exposure to hydrogen peroxide exhibited significantly elevated levels of DNA damage and yet continued to express an enhanced capacity for sperm-oocyte fusion.
Journal ArticleDOI
DNA Integrity in Human Spermatozoa: Relationships With Semen Quality
TL;DR: It is concluded that a significant proportion of infertile men have elevated levels of DNA damage in their ejaculated spermatozoa.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
A simple technique for quantitation of low levels of DNA damage in individual cells
TL;DR: Human lymphocytes were exposed to X-irradiation or treated with H2O2 and the extent of DNA migration was measured using a single-cell microgel electrophoresis technique under alkaline conditions and this technique appears to be sensitive and useful for detecting damage and repair in single cells.
Journal ArticleDOI
The glomerular permeability determined by dextran clearance using Sephadex gel filtration.
TL;DR: A method is described for measuring glomerular permeability by dextran clearance using Sephadex gel filtration technique, and clearance is shown to be a function of molecular size, decreasing with increasing molecular weight.
Journal ArticleDOI
Fractionation of DNA from mammalian cells by alkaline elution
TL;DR: The method of alkaline elution provides a sensitive measure of DNA single-strand length distribution in mamalian cells and is applicable to a variety of problems concerning DNA damage, repair, and replication.
Related Papers (5)
Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells.
O. Ostling,K.J. Johanson +1 more