Journal ArticleDOI
Advances in genetic improvement of Camelina sativa for biofuel and industrial bio-products
Manish Sainger,Anjali Jaiwal,Poonam Ahlawat Sainger,Darshna Chaudhary,Ranjana Jaiwal,Pawan K. Jaiwal +5 more
Reads0
Chats0
TLDR
This review extensively analyses the recent advances and challenges in using molecular markers, genomics, transcriptomics, miRNAs and transgenesis for improvement in biotic and abiotic stresses, carbon assimilation capabilities, seed yield, oil content and composition in camelina for biodiesel fuel properties, nutrition and high value-added industrial products like bioplastics, wax esters and terpenoids.Abstract:
Ever-increasing global energy demand, diminishing fossil fuel reserves and environmental concerns have forced to look for renewable and sustainable alternative energy sources preferentially from non-food crops. Camelina being a short-duration, low-cost, non-food oilseed crop with high content of oil (45%) rich in unsaturated fatty acids and capable of growing in marginal lands has emerged as a potential alternative for biofuel (with low carbon emission) and industrial bio-products. However, the fatty acid profile needs to be refined to make it more efficient for biodiesel and bio-products. Attempts to improve crop yield, oil content and composition through conventional and mutation breeding have been limited due to inadequate genetic diversity and availability of mutants. Simple and easy transformation and recent upsurge in ‘omics’ data (trancriptomics and genomics) has resulted in better understanding of lipid biosynthesis and its regulation, and thus has made it possible to produce unusual lipids with modified fatty acids for new functionalities. However, further improvement is still awaited for carbon assimilation efficiency, resistance to various abiotic and biotic stresses, seed yield, oil content and composition. This review extensively analyses the recent advances and challenges in using molecular markers, genomics, transcriptomics, miRNAs and transgenesis for improvement in biotic and abiotic stresses, carbon assimilation capabilities, seed yield, oil content and composition in camelina for biodiesel fuel properties, nutrition and high value-added industrial products like bioplastics, wax esters and terpenoids.read more
Citations
More filters
Journal ArticleDOI
Genetic Diversity and Population Structure of a Camelina sativa Spring Panel.
Zinan Luo,Jordan R. Brock,John M. Dyer,Toni M. Kutchan,Daniel P. Schachtman,Megan M. Augustin,Yufeng Ge,Noah Fahlgren,Hussein Abdel-Haleem +8 more
TL;DR: Findings provide important information for future allele/gene identification using genome-wide association studies (GWAS) and marker-assisted selection (MAS) to enhance genetic gain in C. sativa breeding programs.
Journal ArticleDOI
Camelina sativa, an oilseed at the nexus between model system and commercial crop.
Malik Meghna,Jihong Tang,Nirmala Sharma,Claire Burkitt,Yuanyuan Ji,Marie Mykytyshyn,Karen Bohmert-Tatarev,Oliver P. Peoples,Kristi D. Snell +8 more
TL;DR: The ability to quickly engineer Camelina with novel traits, advance generations, and bulk up homozygous lines for small-scale field tests in less than a year, in the authors' opinion, far outweighs the complexities associated with the crop.
Journal ArticleDOI
Analysis of yield and genetic similarity of Polish and Ukrainian Camelina sativa genotypes
Danuta Kurasiak-Popowska,Agnieszka Tomkowiak,Magdalena Człopińska,Jan Bocianowski,Dorota Weigt,Jerzy Nawracała +5 more
TL;DR: The yield from currently grown Polish spring cultivars is much higher than the yield from Ukrainian cultivars and the genetic similarity of the Polish and Ukrainian spring genotypes was greater than the similarity ofThe winter genotypes and the camelina mutation lines.
Journal ArticleDOI
Fueling the future; plant genetic engineering for sustainable biodiesel production
TL;DR: The intent of the present review paper is to review and critically discuss the recent genetic and metabolic engineering strategies developed to overcome the shortcoming faced in nonedible plants, including Jatropha curcas and Camelina sativa, as emerging platforms for biodiesel production.
Journal ArticleDOI
Seed yield and oil quality as affected by camelina cultivar and planting date.
Eric Obeng,Eric Obeng,Augustine K. Obour,Nathan O. Nelson,Jose A. Moreno,Ignacio A. Ciampitti,Donghai Wang,Timothy P. Durrett +7 more
TL;DR: Early- to mid-April is the best-planting window for optimum spring camelina stands and seed yield in this environment after heat stress in the growing season and increase in precipitation amounts improved seed yield, oil, PUFA, and linolenic acid concentrations.
References
More filters
Journal ArticleDOI
Purification of a Jojoba Embryo Wax Synthase, Cloning of its cDNA, and Production of High Levels of Wax in Seeds of Transgenic Arabidopsis
Kathryn D. Lardizabal,James G. Metz,Tetsuo Sakamoto,William C. Hutton,Michael R. Pollard,Michael Lassner +5 more
TL;DR: Wax synthase (WS, fatty acyl-coenzyme A [coA]: fatty alcohol acyltransferase) catalyzes the final step in the synthesis of linear esters (waxes) that accumulate in seeds of jojoba (Simmondsia chinensis).
Journal ArticleDOI
The very-long-chain hydroxy fatty acyl-CoA dehydratase PASTICCINO2 is essential and limiting for plant development.
Liên Bach,Louise V. Michaelson,Richard P. Haslam,Yannick Bellec,Lionel Gissot,Jessica Marion,Marco Da Costa,Jean Pierre Boutin,Martine Miquel,Frédérique Tellier,Frédéric Domergue,Jonathan E. Markham,Frédéric Beaudoin,Johnathan A. Napier,Jean-Denis Faure +14 more
TL;DR: It is demonstrated by in vivo bimolecular fluorescence complementation that PAS2 was specifically associated in the endoplasmic reticulum with the enoyl-CoA reductase CER10, the fourth enzyme of the elongase complex, indicating premature termination of fatty acid elongation and confirming the role of PAS 2 in this process.
Journal ArticleDOI
Extending the story of very-long-chain fatty acid elongation.
Tegan M. Haslam,Ljerka Kunst +1 more
TL;DR: Identification and characterization of enzymes in Arabidopsis thaliana has revealed that three of the four enzymes act as generalists, contributing to all metabolic pathways that require VLCFAs.
Journal ArticleDOI
The compatibility of potential bioderived fuels with Jet A-1 aviation kerosene.
TL;DR: In this paper, nine potential bio-fuels derived from sustainable sources were tested for their compatibility with Jet A-1 aviation kerosene, and the effect of each fuel on the range vs. the payload, relative to Jet A -1 was determined using a simplified model in order to ascertain likely impact of adoption upon airline operations.
Journal ArticleDOI
Functional Characterization of the Arabidopsis β-Ketoacyl-Coenzyme A Reductase Candidates of the Fatty Acid Elongase
Frédéric Beaudoin,Xianzhong Wu,Fengling Li,Richard P. Haslam,Jonathan E. Markham,Huanquan Zheng,Huanquan Zheng,Johnathan A. Napier,Ljerka Kunst +8 more
TL;DR: Morphological and biochemical characterization of transgenic lines expressing RNA interference and overexpression constructs targeted against AtKCR1 confirmed that suppressed KCR activity results in a reduction of cuticular wax load and affects VLCFA composition of sphingolipids, seed triacylglycerols, and root glycerolips, demonstrating in planta that KCR is involved in elongation reactions supplying V LCFA for all these diverse classes of lipids.