Camelina sativa, an oilseed at the nexus between model system and commercial crop.
Malik Meghna,Jihong Tang,Nirmala Sharma,Claire Burkitt,Yuanyuan Ji,Marie Mykytyshyn,Karen Bohmert-Tatarev,Oliver P. Peoples,Kristi D. Snell +8 more
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TLDR
The ability to quickly engineer Camelina with novel traits, advance generations, and bulk up homozygous lines for small-scale field tests in less than a year, in the authors' opinion, far outweighs the complexities associated with the crop.Abstract:
The rapid assessment of metabolic engineering strategies in plants is aided by crops that provide simple, high throughput transformation systems, a sequenced genome, and the ability to evaluate the resulting plants in field trials. Camelina sativa provides all of these attributes in a robust oilseed platform. The ability to perform field evaluation of Camelina is a useful, and in some studies essential benefit that allows researchers to evaluate how traits perform outside the strictly controlled conditions of a greenhouse. In the field the plants are subjected to higher light intensities, seasonal diurnal variations in temperature and light, competition for nutrients, and watering regimes dictated by natural weather patterns, all which may affect trait performance. There are difficulties associated with the use of Camelina. The current genetic resources available for Camelina pale in comparison to those developed for the model plant Arabidopsis thaliana; however, the sequence similarity of the Arabidopsis and Camelina genomes often allows the use of Arabidopsis as a reference when additional information is needed. Camelina’s genome, an allohexaploid, is more complex than other model crops, but the diploid inheritance of its three subgenomes is straightforward. The need to navigate three copies of each gene in genome editing or mutagenesis experiments adds some complexity but also provides advantages for gene dosage experiments. The ability to quickly engineer Camelina with novel traits, advance generations, and bulk up homozygous lines for small-scale field tests in less than a year, in our opinion, far outweighs the complexities associated with the crop.read more
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The metabolic origins of non-photorespiratory CO2 release during photosynthesis: a metabolic flux analysis.
TL;DR: In this paper, the authors mapped metabolic fluxes in photosynthesizing source leaves of the oilseed crop and model plant camelina (Camelina sativa), and performed a flux analysis using isotopic labeling patterns of central metabolites during 13CO2 labeling time course, gas exchange and carbohydrate production rate experiments.
Journal ArticleDOI
Metabolic Engineering a Model Oilseed Camelina sativa for the Sustainable Production of High-Value Designed Oils.
Lixia Yuan,Runzhi Li +1 more
TL;DR: This review mainly highlights the latest advance in metabolic engineering towards the predictive manipulation of metabolism for commercial production of desirable bio-based products using camelina as an ideal platform and deeply analysis camelina seed metabolic engineering strategy and its promising achievements.
Journal ArticleDOI
Increasing Monounsaturated Fatty Acid Contents in Hexaploid Camelina sativa Seed Oil by FAD2 Gene Knockout Using CRISPR-Cas9.
Kyeong-Ryeol Lee,Inhwa Jeon,Hami Yu,Sang-Gyu Kim,Hyunsung Kim,Sung-Ju Ahn,Juho Lee,Seon-Kyeong Lee,Hyun Uk Kim +8 more
TL;DR: In this article, the authors used CRISPR-Cas9-mediated gene editing to increase the MUFA contents of Camelina seed oil, which is used as a basis for the metabolic engineering of genes that affect growth in polyploid crops through genome editing.
Journal ArticleDOI
High level accumulation of EPA and DHA in field‐grown transgenic Camelina – a multi‐territory evaluation of TAG accumulation and heterogeneity
Lihua Han,Sarah Usher,Sjur Sandgrind,Kirsty L. Hassall,Olga Sayanova,Louise V. Michaelson,Richard P. Haslam,Johnathan A. Napier +7 more
TL;DR: Examination of non‐seed tissues for the unintended accumulation of EPA and DHA failed to identify their presence in leaf, stem, flower, anther or capsule shell material, confirming the seed‐specific accumulation of these novel fatty acids.
Journal ArticleDOI
Acclimation in plants – the Green Hub consortium
Tatjana Kleine,Thomas Nägele,H. Ekkehard Neuhaus,Christian Schmitz-Linneweber,Alisdair R. Fernie,Peter Geigenberger,Bernhard Grimm,Kerstin Kaufmann,Edda Klipp,Jörg Meurer,Torsten Möhlmann,Timo Mühlhaus,Belén Naranjo,Joerg Nickelsen,Andreas Richter,Hannes Ruwe,Michael Schroda,Serena Schwenkert,Oliver Trentmann,Felix Willmund,Reimo Zoschke,Dario Leister +21 more
TL;DR: In this paper, the authors highlight the current state of research on the role of gene expression, metabolism and signalling in acclimation, with a focus on chloroplast-related processes.
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