The emerging biofuel crop Camelina sativa retains a highly undifferentiated hexaploid genome structure
Sateesh Kagale,Sateesh Kagale,Chushin Koh,John Nixon,Venkatesh Bollina,Wayne E. Clarke,Reetu Tuteja,Charles Spillane,Stephen J. Robinson,Matthew G. Links,Carling Clarke,Erin E. Higgins,Terry Huebert,Andrew G. Sharpe,Isobel A. P. Parkin +14 more
Reads0
Chats0
TLDR
The first chromosome-scale high-quality reference genome sequence is generated for C. sativa and annotated 89,418 protein-coding genes, representing a whole-genome triplication event relative to the crucifer model Arabidopsis thaliana.Abstract:
Camelina sativa is an oilseed with desirable agronomic and oil-quality attributes for a viable industrial oil platform crop. Here we generate the first chromosome-scale high-quality reference genome sequence for C. sativa and annotated 89,418 protein-coding genes, representing a whole-genome triplication event relative to the crucifer model Arabidopsis thaliana. C. sativa represents the first crop species to be sequenced from lineage I of the Brassicaceae. The well-preserved hexaploid genome structure of C. sativa surprisingly mirrors those of economically important amphidiploid Brassica crop species from lineage II as well as wheat and cotton. The three genomes of C. sativa show no evidence of fractionation bias and limited expression-level bias, both characteristics commonly associated with polyploid evolution. The highly undifferentiated polyploid genome of C. sativa presents significant consequences for breeding and genetic manipulation of this industrial oil crop.read more
Citations
More filters
Journal Article
The Design and Analysis of Experiments
TL;DR: This book by a teacher of statistics (as well as a consultant for "experimenters") is a comprehensive study of the philosophical background for the statistical design of experiment.
Journal ArticleDOI
Sequencing of allotetraploid cotton ( Gossypium hirsutum L. acc. TM-1) provides a resource for fiber improvement
Tianzhen Zhang,Yan Hu,Wenkai Jiang,Lei Fang,Xueying Guan,Jiedan Chen,Jinbo Zhang,Christopher A. Saski,Brian E. Scheffler,David M. Stelly,Amanda M. Hulse-Kemp,Qun Wan,Bingliang Liu,Chunxiao Liu,Sen Wang,Mengqiao Pan,Yangkun Wang,Dawei Wang,Wenxue Ye,Lijing Chang,Wenpan Zhang,Qingxin Song,Ryan C. Kirkbride,Xiao-Ya Chen,Elizabeth S. Dennis,Danny J. Llewellyn,Daniel G. Peterson,Peggy Thaxton,Don C. Jones,Qiong Wang,Xiaoyang Xu,Hua Zhang,Huaitong Wu,Lei Zhou,Gaofu Mei,Shuqi Chen,Yue Tian,Dan Xiang,Xinghe Li,Jian Ding,Qiyang Zuo,Linna Tao,Yunchao Liu,Ji Li,Yu Lin,Yuanyuan Hui,Zhisheng Cao,Caiping Cai,Xiefei Zhu,Zhi Jiang,Baoliang Zhou,Wangzhen Guo,Ruiqiang Li,Z. Jeffrey Chen +53 more
TL;DR: Genomic signatures of selection and domestication are associated with positively selected genes (PSGs) for fiber improvement in the A subgenome and for stress tolerance in the D subgenomes, suggesting asymmetric evolution.
Journal ArticleDOI
Gene duplication and evolution in recurring polyploidization-diploidization cycles in plants.
Xin Qiao,Qionghou Li,Hao Yin,Kaijie Qi,Leiting Li,Runze Wang,Shaoling Zhang,Andrew H. Paterson +7 more
TL;DR: A comprehensive landscape of different modes of gene duplication across the plant kingdom is identified by comparing 141 genomes, which provides a solid foundation for further investigation of the dynamic evolution of duplicate genes.
Journal ArticleDOI
Transcriptome and methylome profiling reveals relics of genome dominance in the mesopolyploid Brassica oleracea.
Isobel A. P. Parkin,Chushin Koh,Haibao Tang,Stephen J. Robinson,Sateesh Kagale,Sateesh Kagale,Wayne E. Clarke,Christopher D. Town,John Nixon,Vivek Krishnakumar,Shelby L. Bidwell,Harry Belcram,Matthew G. Links,Jérémy Just,Carling Clarke,Tricia Bender,Terry Huebert,Annaliese S. Mason,Chris J Pires,Guy C. Barker,Jonathan D. Moore,Peter Glen Walley,Sahana Manoli,Jacqueline Batley,David Edwards,Matthew N. Nelson,Xiyin Wang,Andrew H. Paterson,Graham J.W. King,Ian Bancroft,Boulos Chalhoub,Andrew G. Sharpe +31 more
TL;DR: Differential expression of the triplicated syntelogs and cytosine methylation levels across the sub-genomes suggest residual marks of the genome dominance that led to the current genome architecture, and epigenetic mechanisms play a role in the functional diversification of duplicate genes.
Journal ArticleDOI
Advances in the understanding of cuticular waxes in Arabidopsis thaliana and crop species
Saet Buyl Lee,Mi Chung Suh +1 more
TL;DR: Recent advances in the understanding of the biological functions of genes involved in cuticular wax biosynthesis, transport, and regulation of wax deposition from Arabidopsis and crop species are discussed, information on cuticular Wax amounts and composition in various organs of nine representative plant species are provided, and the important issues that need to be investigated are suggested.
References
More filters
Journal ArticleDOI
Basic Local Alignment Search Tool
TL;DR: A new approach to rapid sequence comparison, basic local alignment search tool (BLAST), directly approximates alignments that optimize a measure of local similarity, the maximal segment pair (MSP) score.
Journal ArticleDOI
Full-length transcriptome assembly from RNA-Seq data without a reference genome.
Manfred Grabherr,Brian J. Haas,Moran Yassour,Moran Yassour,Joshua Z. Levin,Dawn Thompson,Ido Amit,Xian Adiconis,Lin Fan,Raktima Raychowdhury,Qiandong Zeng,Zehua Chen,Evan Mauceli,Nir Hacohen,Andreas Gnirke,Nicholas Rhind,Federica Di Palma,Bruce W. Birren,Chad Nusbaum,Kerstin Lindblad-Toh,Kerstin Lindblad-Toh,Nir Friedman,Aviv Regev +22 more
TL;DR: The Trinity method for de novo assembly of full-length transcripts and evaluate it on samples from fission yeast, mouse and whitefly, whose reference genome is not yet available, providing a unified solution for transcriptome reconstruction in any sample.
Journal Article
The Design and Analysis of Experiments
TL;DR: This book by a teacher of statistics (as well as a consultant for "experimenters") is a comprehensive study of the philosophical background for the statistical design of experiment.
Journal ArticleDOI
Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks
Cole Trapnell,Adam Roberts,Loyal A. Goff,Loyal A. Goff,Loyal A. Goff,Geo Pertea,Daehwan Kim,Daehwan Kim,David R. Kelley,David R. Kelley,Harold Pimentel,Steven L. Salzberg,John L. Rinn,John L. Rinn,Lior Pachter +14 more
TL;DR: This protocol begins with raw sequencing reads and produces a transcriptome assembly, lists of differentially expressed and regulated genes and transcripts, and publication-quality visualizations of analysis results, which takes less than 1 d of computer time for typical experiments and ∼1 h of hands-on time.
Journal ArticleDOI
Versatile and open software for comparing large genomes
Stefan Kurtz,Adam M. Phillippy,Arthur L. Delcher,Michael E. Smoot,Martin Shumway,Corina Antonescu,Steven L. Salzberg +6 more
TL;DR: The newest version of MUMmer easily handles comparisons of large eukaryotic genomes at varying evolutionary distances, as demonstrated by applications to multiple genomes.
Related Papers (5)
Generation of transgenic plants of a potential oilseed crop Camelina sativa by Agrobacterium-mediated transformation
Chaofu Lu,Jinling Kang +1 more
The Brassica oleracea genome reveals the asymmetrical evolution of polyploid genomes
Shengyi Liu,Yumei Liu,Xinhua Yang,Chaobo Tong,David Edwards,Isobel A. P. Parkin,Meixia Zhao,Jianxin Ma,Jingyin Yu,Shunmou Huang,Xiyin Wang,Junyi Wang,Kun Lu,Zhiyuan Fang,Ian Bancroft,Tae-Jin Yang,Qiong Hu,Xinfa Wang,Zhen Yue,Haojie Li,Linfeng Yang,Jian Wu,Qing Zhou,Wanxin Wang,Graham J.W. King,J. Chris Pires,Changxin Lu,Zhangyan Wu,Perumal Sampath,Zhuo Wang,Hui Guo,Shengkai Pan,Limei Yang,Jiumeng Min,Dong Zhang,Dianchuan Jin,Wanshun Li,Harry Belcram,Jinxing Tu,Mei Guan,Cunkou Qi,Dezhi Du,Jiana Li,Liangcai Jiang,Jacqueline Batley,Andrew G. Sharpe,Beom Seok Park,Pradeep Ruperao,Feng Cheng,Nomar Espinosa Waminal,Yin Huang,Caihua Dong,Li Wang,Jingping Li,Zhiyong Hu,Mu Zhuang,Yi Huang,Junyan Huang,Jiaqin Shi,Desheng Mei,Jing Liu,Tae-Ho Lee,Jinpeng Wang,Huizhe Jin,Zaiyun Li,Xun Li,Jiefu Zhang,Lu Xiao,Yongming Zhou,Zhongsong Liu,Xuequn Liu,Rui Qin,Xu Tang,Wenbin Liu,Yupeng Wang,Yangyong Zhang,Jonghoon Lee,Hyun Hee Kim,Xun Xu,Xinming Liang,Wei Hua,Xiaowu Wang,Jun Wang,Boulos Chalhoub,Andrew H. Paterson +84 more
The genome of the mesopolyploid crop species Brassica rapa
Xiaowu Wang,Hanzhong Wang,Jun Wang,Jun Wang,Jun Wang,Rifei Sun,Jian Wu,Shengyi Liu,Yinqi Bai,Jeong-Hwan Mun,Ian Bancroft,Feng Cheng,Sanwen Huang,Xixiang Li,Wei Hua,Junyi Wang,Xiyin Wang,Xiyin Wang,Michael Freeling,J. Chris Pires,Andrew H. Paterson,Boulos Chalhoub,Bo Wang,Alice Hayward,Alice Hayward,Andrew G. Sharpe,Beom-Seok Park,Bernd Weisshaar,Binghang Liu,Bo Li,Bo Liu,Chaobo Tong,Chi Song,Chris Duran,Chris Duran,Chunfang Peng,Geng Chunyu,Chushin Koh,Chuyu Lin,David Edwards,David Edwards,Desheng Mu,Di Shen,Eleni Soumpourou,Fei Li,Fiona Fraser,Gavin C. Conant,Gilles Lassalle,Graham J.W. King,Guusje Bonnema,Haibao Tang,Haiping Wang,Harry Belcram,Heling Zhou,Hideki Hirakawa,Hiroshi Abe,Hui Guo,Hui Wang,Huizhe Jin,Isobel A. P. Parkin,Jacqueline Batley,Jacqueline Batley,Jeong-Sun Kim,Jérémy Just,Jianwen Li,Jiaohui Xu,Jie Deng,Jin A Kim,Jingping Li,Jingyin Yu,Jinling Meng,Jinpeng Wang,Jiumeng Min,Julie Poulain,Katsunori Hatakeyama,Kui Wu,Li Wang,Lu Fang,Martin Trick,Matthew G. Links,Meixia Zhao,Mina Jin,Nirala Ramchiary,Nizar Drou,Paul J. Berkman,Paul J. Berkman,Qingle Cai,Quanfei Huang,Ruiqiang Li,Satoshi Tabata,Shifeng Cheng,Shu Zhang,Shujiang Zhang,Shunmou Huang,Shusei Sato,Silong Sun,Soo-Jin Kwon,Su-Ryun Choi,Tae-Ho Lee,Wei Fan,Xiang Zhao,Xu Tan,Xun Xu,Yan Wang,Yang Qiu,Ye Yin,Yingrui Li,Yongchen Du,Yongcui Liao,Yong Pyo Lim,Yoshihiro Narusaka,Yupeng Wang,Zhenyi Wang,Zhenyu Li,Zhiwen Wang,Zhiyong Xiong,Zhonghua Zhang +116 more
Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome
Boulos Chalhoub,Shengyi Liu,Isobel A. P. Parkin,Haibao Tang,Haibao Tang,Xiyin Wang,Julien Chiquet,Harry Belcram,Chaobo Tong,Birgit Samans,Margot Correa,Corinne Da Silva,Jérémy Just,Cyril Falentin,Chu Shin Koh,Isabelle Le Clainche,Maria Bernard,Pascal Bento,Benjamin Noel,Karine Labadie,Adriana Alberti,Mathieu Charles,Dominique Arnaud,Hui Guo,Christian Daviaud,Salman Alamery,Kamel Jabbari,Kamel Jabbari,Meixia Zhao,Patrick P. Edger,Houda Chelaifa,David C. Tack,Gilles Lassalle,Imen Mestiri,Nicolas Schnel,Marie-Christine Le Paslier,Guangyi Fan,Victor Renault,Philippe E. Bayer,Agnieszka A. Golicz,Sahana Manoli,Tae-Ho Lee,Vinh Ha Dinh Thi,Smahane Chalabi,Qiong Hu,Chuchuan Fan,Reece Tollenaere,Yunhai Lu,Christophe Battail,Jinxiong Shen,Christine Sidebottom,Xinfa Wang,Aurélie Canaguier,Aurélie Chauveau,Aurélie Bérard,G. Deniot,Mei Guan,Zhongsong Liu,Fengming Sun,Yong Pyo Lim,Eric Lyons,Christopher D. Town,Ian Bancroft,Xiaowu Wang,Jinling Meng,Jianxin Ma,J. Chris Pires,Graham J.W. King,Dominique Brunel,Régine Delourme,Michel Renard,Jean-Marc Aury,Keith L. Adams,Jacqueline Batley,Jacqueline Batley,Rod J. Snowdon,Jörg Tost,David Edwards,David Edwards,Yongming Zhou,Wei Hua,Andrew G. Sharpe,Andrew H. Paterson,Chunyun Guan,Patrick Wincker,Patrick Wincker,Patrick Wincker +86 more