Advances in multiphoton microscopy technology
Erich Hoover,Jeff Squier +1 more
TLDR
The basic architecture of a multiphoton microscope capable of real-time analysis of cellular structure and function is discussed and the state-of-the-art technologies for the quantitative imaging of biological phenomena are summarized.Abstract:
The ability to dynamically image features deep within living organisms, permitting real-time analysis of cellular structure and function, is important for biological science. This Review article discusses multiphoton microscopy capable of such analysis, along with technologies that are pushing the limits of phenomena that can be quantitatively imaged.read more
Citations
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Fluorescent Sensors for Measuring Metal Ions in Living Systems
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Near-infrared fluorophores for biomedical imaging
TL;DR: This Review covers recent progress on near-infrared fluorescence imaging for preclinical animal studies and clinical diagnostics and interventions.
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Two-Photon-Pumped Perovskite Semiconductor Nanocrystal Lasers.
Yanqing Xu,Qi Chen,Chunfeng Zhang,Rui Wang,Hua Wu,Xiaoyu Zhang,Guichuan Xing,William W. Yu,Xiaoyong Wang,Yu Zhang,Min Xiao +10 more
TL;DR: Stable two-photon-pumped lasing is demonstrated at a remarkable low threshold by coupling CsPbBr3 nanocrystals with microtubule resonators and these findings suggest perovskite nanocry crystals can be used as excellent gain medium for high-performance frequency-up-conversion lasers toward practical applications.
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3D cell culture systems modeling tumor growth determinants in cancer target discovery
TL;DR: This review discusses the cellular, chemical and physical factors contributing to zonation and cellular crosstalk within tumor masses and describes 3D cell culture technologies for growth of MCTS as advanced tools for exploring molecular tumor growth determinants and facilitating drug discovery efforts.
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Recent advances in fibre lasers for nonlinear microscopy
Chris Xu,Frank W. Wise +1 more
TL;DR: Recent advances are leading to high-performance sources with the potential for robust, inexpensive, integrated instruments, and challenges and opportunities for fiber lasers in nonlinear bioimaging are identified.
References
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Two-Photon Laser Scanning Fluorescence Microscopy
TL;DR: The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation.
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Imaging intracellular fluorescent proteins at nanometer resolution.
Eric Betzig,George H. Patterson,Rachid Sougrat,O. Wolf Lindwasser,Scott G. Olenych,Juan S. Bonifacino,Michael W. Davidson,Jennifer Lippincott-Schwartz,Harald F. Hess +8 more
TL;DR: This work introduced a method for optically imaging intracellular proteins at nanometer spatial resolution and used this method to image specific target proteins in thin sections of lysosomes and mitochondria and in fixed whole cells to image retroviral protein Gag at the plasma membrane.
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Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy
Stefan W. Hell,Jan Wichmann +1 more
TL;DR: A new type of scanning fluorescence microscope capable of resolving 35 nm in the far field is proposed, overcome the diffraction resolution limit by employing stimulated emission to inhibit the fluorescence process in the outer regions of the excitation point-spread function.
Journal Article
Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy
Stefan W. Hell,Jan Wichmann +1 more
TL;DR: In this paper, the authors proposed a new type of scanning fluorescence microscope capable of resolving 35 nm in the far field by employing stimulated emission to inhibit the fluorescence process in the outer regions of the excitation point spread function.
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Deep tissue two-photon microscopy
Fritjof Helmchen,Winfried Denk +1 more
TL;DR: Fundamental concepts of nonlinear microscopy are reviewed and conditions relevant for achieving large imaging depths in intact tissue are discussed.