Journal ArticleDOI
Agrobacterium tumefaciens-mediated transformation of filamentous fungi.
TLDR
It is reported that A. tumefaciens can also transfer its T-DNA efficiently to the filamentous fungus Aspergillus awamori, demonstrating DNA transfer between a prokaryote and a filamentous fungi.Abstract:
Agrobacterium tumefaciens transfers part of its Ti plasmid, the T-DNA, to plant cells during tumorigenesis. It is routinely used for the genetic modification of a wide range of plant species. We report that A. tumefaciens can also transfer its T-DNA efficiently to the filamentous fungus Aspergillus awamori, demonstrating DNA transfer between a prokaryote and a filamentous fungus. We transformed both protoplasts and conidia with frequencies that were improved up to 600-fold as compared with conventional techniques for transformation of A. awamori protoplasts. The majority of the A. awamori transformants contained a single T-DNA copy randomly integrated at a chromosomal locus. The T-DNA integrated into the A. awamori genome in a manner similar to that described for plants. We also transformed a variety of other filamentous fungi, including Aspergillus niger, Fusarium venenatum, Trichoderma reesei, Colletotrichum gloeosporioides, Neurospora crassa, and the mushroom Agaricus bisporus, demonstrating that transformation using A. tumefaciens is generally applicable to filamentous fungi.read more
Citations
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Journal ArticleDOI
Agrobacterium-Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool
TL;DR: Knowledge of fundamental biological principles embracing both the host and the pathogen have been and will continue to be key to extending the utility of Agrobacterium for genetic engineering purposes.
Journal ArticleDOI
The Genome of the Natural Genetic Engineer Agrobacterium tumefaciens C58
Derek W. Wood,João C. Setubal,Rajinder Kaul,Dave E. Monks,João Paulo Kitajima,Vagner K. Okura,Yang Zhou,Lishan Chen,Gwendolyn E. Wood,Nalvo F. Almeida,Lisa Woo,Yuching Chen,Ian T. Paulsen,Jonathan A. Eisen,Peter D. Karp,Donald Bovee,Peter Chapman,James B. Clendenning,Glenda Deatherage,Will Gillet,Charles E. Grant,Tatyana Kutyavin,Ruth Levy,Meng-Jin Li,Erin K. McClelland,Anthony Palmieri,Christopher K. Raymond,Gregory Rouse,Channakhone Saenphimmachak,Zaining Wu,Pedro Romero,David E. Gordon,Shiping Zhang,Heayun Yoo,Yumin Tao,Phyllis Biddle,Mark Timothy Jung,William Krespan,Michael Perry,Bill Gordon-Kamm,Li Liao,Sun Kim,Carol A. Hendrick,Zuo-Yu Zhao,Maureen Dolan,Forrest Chumley,Scott V. Tingey,Jean-Francois Tomb,Milton P. Gordon,Maynard V. Olson,Eugene W. Nester +50 more
TL;DR: The 5.67-megabase genome of the plant pathogen Agrobacterium tumefaciens C58 consists of a circular chromosome, a linear chromosome, and two plasmids that suggest a recent evolutionary divergence.
Journal ArticleDOI
Bacterial-Fungal Interactions: Hyphens between Agricultural, Clinical, Environmental, and Food Microbiologists
Pascale Frey-Klett,P. Burlinson,Aurélie Deveau,Matthieu Barret,Mika T. Tarkka,Alain Sarniguet +5 more
TL;DR: It is shown that in many cases, parallels can be drawn between different scenarios in which bacterial-fungal interactions are important, and how new avenues of investigation may enhance the ability to combat, manipulate, or exploit bacterial- fungal complexes for the economic and practical benefit of humanity.
Journal ArticleDOI
Agrobacterium-mediated transformation of Fusarium oxysporum: An efficient tool for insertional mutagenesis and gene transfer
TL;DR: The construction of four novel binary vectors for fungal transformation and the optimization of an ATMT protocol for insertional mutagenesis, which permits an efficient genetic manipulation of Fusarium oxysporum and other phytopathogenic fungi to be achieved.
Journal ArticleDOI
Bacterial type IV secretion: conjugation systems adapted to deliver effector molecules to host cells.
TL;DR: Several bacterial pathogens utilize conjugation machines to export effector molecules during infection, including Bordetella pertussis, Legionella pneumophila, Brucellaspp.
References
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Journal ArticleDOI
Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA.
TL;DR: A large number of morphologically normal, fertile, transgenic rice plants were obtained by co-cultivation of rice tissues with Agrobacterium tumefaciens, and sequence analysis revealed that the boundaries of the T-DNA in transgenic Rice plants were essentially identical to those intransgenic dicotyledons.
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Binary Agrobacterium vectors for plant transformation
TL;DR: A vector molecule for the efficient transformation of higher plants has been constructed with several features that make it efficient to use.
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High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens
TL;DR: Transformants of maize inbred A188 were efficiently produced from immature embryos cocultivated with Agrobacterium tumefaciens that carried “super-binary” vectors that carried stable integration, expression, and inheritance of the transgenes.
Journal ArticleDOI
Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli.
TL;DR: A new, heterologous, dominant marker for selection of Aspergillus transformants is described, based on the Escherichia coli hygromycin B (HmB) phosphotransferase gene (hph), which confers HmB resistance to As pergillus species.
Journal ArticleDOI
Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae.
TL;DR: The results showed that T‐DNA circles were formed in yeast with precise fusions between the left and right borders, suggesting that the process of T‐ DNA integration is predominantly determined by host factors.