An estimate of the total number of true human miRNAs.
Julia Alles,Tobias Fehlmann,Ulrike Fischer,Christina Backes,Galata,Marie Minet,Martin Hart,Masood Abu-Halima,Friedrich A. Grässer,Hans-Peter Lenhof,Andreas Keller,Eckart Meese +11 more
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TLDR
The experimentally validated miRNAs will contribute to revising targetomes hypothesized by utilizing falsely annotated miRNA candidates, 1115 of which are currently annotated in miRBase V22.Abstract:
While the number of human miRNA candidates continuously increases, only a few of them are completely characterized and experimentally validated. Toward determining the total number of true miRNAs, we employed a combined in silico high- and experimental low-throughput validation strategy. We collected 28 866 human small RNA sequencing data sets containing 363.7 billion sequencing reads and excluded falsely annotated and low quality data. Our high-throughput analysis identified 65% of 24 127 mature miRNA candidates as likely false-positives. Using northern blotting, we experimentally validated miRBase entries and novel miRNA candidates. By exogenous overexpression of 108 precursors that encode 205 mature miRNAs, we confirmed 68.5% of the miRBase entries with the confirmation rate going up to 94.4% for the high-confidence entries and 18.3% of the novel miRNA candidates. Analyzing endogenous miRNAs, we verified the expression of 8 miRNAs in 12 different human cell lines. In total, we extrapolated 2300 true human mature miRNAs, 1115 of which are currently annotated in miRBase V22. The experimentally validated miRNAs will contribute to revising targetomes hypothesized by utilizing falsely annotated miRNAs.read more
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Non-Coding RNAs Improve the Predictive Power of Network Medicine
TL;DR: Barabasi et al. as discussed by the authors proposed a network science Institute and Department of Physics, Northeastern University, Boston, MA 02115, USA;2 Channing Division of Network Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston University, Massachusetts, USA.
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The effects of all trans retinoic acid, vitamin D3 and their combination on plasma levels of miRNA-125a-5p, miRNA-34a, and miRNA-126in an experimental model of diabetes
Mohammad Sharifzadeh,Aghil Esmaeili-Bandboni,Mohammad Reza Emami,Fatemeh Naeini,Meysam Zarezadeh,Mohammad Hassan Javanbakht +5 more
TL;DR: Vitamin D3+ATRA can be considered a new therapeutic agent that can elevate miR-126 expression and prevent diabetes-related cardiovascular complications and reduce the circulating levels of miR (MicroRNAs) in diabetic rats.
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Noncoding-RNA-Based Therapeutics with an Emphasis on Prostatic Carcinoma—Progress and Challenges
TL;DR: The role of ncRNA therapeutics in prostatic carcinoma is focused on while exploring basic biological aspects of these molecules that represent about 97% of the transcriptome in humans.
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Plasma microRNA-320a as a Potential Biomarker of Physiological Changes during Training in Professional Volleyball Players
Rafał Podgórski,Marek Cieśla,D. Podgórska,Wojciech Bajorek,Artur Płonka,Wojciech Czarny,R. Trybulski,Paweł Król +7 more
TL;DR: Future studies are required to define whether these miRNAs, especially miR-320a, as well as IGF1R expression could be useful biomarkers of physiological changes during exercise and to discover their detailed biological roles in mode-specific exercise training adaptations of professional athletes.
Journal ArticleDOI
The effects of all trans retinoic acid, vitamin D3 and their combination on plasma levels of miRNA-125a-5p, miRNA-34a, and miRNA-126 in an experimental model of diabetes.
TL;DR: In this article , the effects of ATRA (all trans retinoic acid), vitamin D3, and their combination on circulating levels of miR (MicroRNA) -125a-5p, miR-126, and miR34a in diabetic rats were evaluated.
References
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MicroRNAs: Genomics, Biogenesis, Mechanism, and Function
TL;DR: Although they escaped notice until relatively recently, miRNAs comprise one of the more abundant classes of gene regulatory molecules in multicellular organisms and likely influence the output of many protein-coding genes.
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MicroRNAs: Target Recognition and Regulatory Functions
TL;DR: The current understanding of miRNA target recognition in animals is outlined and the widespread impact of miRNAs on both the expression and evolution of protein-coding genes is discussed.
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The functions of animal microRNAs
TL;DR: Evidence is mounting that animal miRNAs are more numerous, and their regulatory impact more pervasive, than was previously suspected.
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Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5
TL;DR: Human embryonic kidney cells have been transformed by exposing cells to sheared fragments of adenovirus type 5 DNA, and the transformed cells exhibited many of the characteristics of transformation including the elaboration of a virus-specific tumour antigen.
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miRBase: annotating high confidence microRNAs using deep sequencing data.
Ana Kozomara,Sam Griffiths-Jones +1 more
TL;DR: An update of the miRBase database is described, including the collation and use of deep sequencing data sets to assign levels of confidence to miR base entries, and a high confidence subset of miR Base entries are provided, based on the pattern of mapped reads.