An estimate of the total number of true human miRNAs.
Julia Alles,Tobias Fehlmann,Ulrike Fischer,Christina Backes,Galata,Marie Minet,Martin Hart,Masood Abu-Halima,Friedrich A. Grässer,Hans-Peter Lenhof,Andreas Keller,Eckart Meese +11 more
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TLDR
The experimentally validated miRNAs will contribute to revising targetomes hypothesized by utilizing falsely annotated miRNA candidates, 1115 of which are currently annotated in miRBase V22.Abstract:
While the number of human miRNA candidates continuously increases, only a few of them are completely characterized and experimentally validated. Toward determining the total number of true miRNAs, we employed a combined in silico high- and experimental low-throughput validation strategy. We collected 28 866 human small RNA sequencing data sets containing 363.7 billion sequencing reads and excluded falsely annotated and low quality data. Our high-throughput analysis identified 65% of 24 127 mature miRNA candidates as likely false-positives. Using northern blotting, we experimentally validated miRBase entries and novel miRNA candidates. By exogenous overexpression of 108 precursors that encode 205 mature miRNAs, we confirmed 68.5% of the miRBase entries with the confirmation rate going up to 94.4% for the high-confidence entries and 18.3% of the novel miRNA candidates. Analyzing endogenous miRNAs, we verified the expression of 8 miRNAs in 12 different human cell lines. In total, we extrapolated 2300 true human mature miRNAs, 1115 of which are currently annotated in miRBase V22. The experimentally validated miRNAs will contribute to revising targetomes hypothesized by utilizing falsely annotated miRNAs.read more
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Signal differentiation models for multiple microRNA detection: a critical review
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miR-199a-5p inhibits the Expression of ABCB11 in Obstructive Cholestasis
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TL;DR: In this paper, a microRNA screen of mouse liver after common bile duct ligation (CBDL) was conducted to determine whether miR-199a-5p contributes to the depletion of ABCB11/Abcb11 in cholestasis in mice.
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Non-Coding RNAs Regulating Mitochondrial Functions and the Oxidative Stress Response as Putative Targets against Age-Related Macular Degeneration (AMD)
TL;DR: In this paper , a review of the regulation of the mitochondrial function and antioxidant stress response by non-coding RNAs (ncRNAs), newly emerged epigenetic factors, is discussed.
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Oral Delivery of miR-320-3p with Lipidic Aminoglycoside Derivatives at Mid-Lactation Alters miR-320-3p Endogenous Levels in the Gut and Brain of Adult Rats According to Early or Regular Weaning
Gabriel Araújo Tavares,Amada Torres,Gwenola Le Drean,M. Queignec,Blandine Castellano,Laurent Tesson,Séverine Remy,Ignacio Anegon,Bruno Pitard,Bertrand Kaeffer +9 more
TL;DR: In this paper , the authors investigated the effect of microRNAs on the gut and brain of young rats according to weaning and found that miR-320-3p was upregulated in the duodenum.
References
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Journal ArticleDOI
MicroRNAs: Genomics, Biogenesis, Mechanism, and Function
TL;DR: Although they escaped notice until relatively recently, miRNAs comprise one of the more abundant classes of gene regulatory molecules in multicellular organisms and likely influence the output of many protein-coding genes.
Journal ArticleDOI
MicroRNAs: Target Recognition and Regulatory Functions
TL;DR: The current understanding of miRNA target recognition in animals is outlined and the widespread impact of miRNAs on both the expression and evolution of protein-coding genes is discussed.
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The functions of animal microRNAs
TL;DR: Evidence is mounting that animal miRNAs are more numerous, and their regulatory impact more pervasive, than was previously suspected.
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Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5
TL;DR: Human embryonic kidney cells have been transformed by exposing cells to sheared fragments of adenovirus type 5 DNA, and the transformed cells exhibited many of the characteristics of transformation including the elaboration of a virus-specific tumour antigen.
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miRBase: annotating high confidence microRNAs using deep sequencing data.
Ana Kozomara,Sam Griffiths-Jones +1 more
TL;DR: An update of the miRBase database is described, including the collation and use of deep sequencing data sets to assign levels of confidence to miR base entries, and a high confidence subset of miR Base entries are provided, based on the pattern of mapped reads.