An Integrated Stress Response Regulates Amino Acid Metabolism and Resistance to Oxidative Stress
Heather P. Harding,Yuhong Zhang,Huiquing Zeng,Isabel Novoa,Phoebe D. Lu,Marcella Calfon,Navid Sadri,Chi Yun,Brian Popko,Richard S. Paules,David F. Stojdl,John C. Bell,Thore Hettmann,Jeffrey M. Leiden,David Ron +14 more
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TLDR
A signaling pathway initiated by eIF2alpha phosphorylation protects cells against metabolic consequences of ER oxidation by promoting the linked processes of amino acid sufficiency and resistance to oxidative stress.About:
This article is published in Molecular Cell.The article was published on 2003-03-01 and is currently open access. It has received 2920 citations till now. The article focuses on the topics: Unfolded protein response & Amino acid import.read more
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Signal integration in the endoplasmic reticulum unfolded protein response
David Ron,Peter Walter +1 more
TL;DR: Together, at least three mechanistically distinct arms of the UPR regulate the expression of numerous genes that function within the secretory pathway but also affect broad aspects of cell fate and the metabolism of proteins, amino acids and lipids.
Journal ArticleDOI
Autophagy and the Integrated Stress Response
TL;DR: Autophagy is a cell biological process that is a central component of the integrated stress response and can be integrated with other cellular stress responses through parallel stimulation of autophagy and other stress responses by specific stress stimuli.
Journal ArticleDOI
The mammalian unfolded protein response
TL;DR: In the endoplasmic reticulum (ER), secretory and transmembrane proteins fold into their native conformation and undergo posttranslational modifications important for their activity and structure as mentioned in this paper.
Journal ArticleDOI
Roles of CHOP/GADD153 in endoplasmic reticulum stress.
TL;DR: The current understanding of the roles of C/EBP homologous protein (CHOP) and GADD153 in ER stress-mediated apoptosis and in diseases including diabetes, brain ischemia and neurodegenerative disease are summarized.
Journal ArticleDOI
IDO expression by dendritic cells: tolerance and tryptophan catabolism.
Andrew L. Mellor,David H. Munn +1 more
TL;DR: This review summarizes key recent developments and proposes a unifying model for the role of IDO in tolerance induction, including studies of mammalian pregnancy, tumour resistance, chronic infections and autoimmune diseases.
References
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Expression monitoring by hybridization to high density oligonucleotide arrays
TL;DR: In this article, the authors proposed a method for monitoring the expression levels of a multiplicity of genes by hybridizing a nucleic acid sample to a high density array of oligonucleotide probes and quantifying the hybridized nucleic acids in the array.
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Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine
TL;DR: It is reported here that 2-vinylpyridine is a much better reagent for the derivitization of glutathione, and it is demonstrated that the total glutATHione concentration in mouse plasma is substantially higher than generally reported and that glutathion disulfide constitutes less than 30% of the totalglutathione present.
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Regulated Translation Initiation Controls Stress-Induced Gene Expression in Mammalian Cells
Heather P. Harding,Isabel Novoa,Yuhong Zhang,Huiqing Zeng,Ronald C. Wek,Matthieu Schapira,David Ron +6 more
TL;DR: Protein kinases that phosphorylate the alpha subunit of eukaryotic initiation factor 2 (eIF2alpha) are activated in stressed cells and negatively regulate protein synthesis, resulting in the induction of the downstream gene CHOP (GADD153).
Journal ArticleDOI
IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA
Marcella Calfon,Huiqing Zeng,Fumihiko Urano,Jeffery H. Till,Stevan R. Hubbard,Heather P. Harding,Scott G. Clark,David Ron +7 more
TL;DR: It is demonstrated that mutations in either ire-1 or the transcription-factor-encoding xbp-1 gene abolished the UPR in Caenorhabditis elegans, suggesting that physiological ER load regulates a developmental decision in higher eukaryotes.
Book ChapterDOI
Determination of glutathione and glutathione disulfide in biological samples.
TL;DR: 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB)-GSSG reductase recycling assay for total glutathione is a specific, sensitive, rapid, and reliable procedure, however, because the method depends on an accurate standard curve, appropriate standards containing the protein precipitating agent are essential.