Journal ArticleDOI
Anchorage-independent growth of primary rat embryo cells is induced by platelet-derived growth factor and inhibited by type-beta transforming growth factor.
Reads0
Chats0
TLDR
The results suggest that the transforming potential of PDGF in an appropriate responsive cell can be controlled not only through its interaction with its own receptor, but also by the presence of inhibitory factors such as TGF‐β.Abstract:
Several growth factors implicated in the process of cellular transformation were tested for their ability to induce anchorage-independent (AI) growth of primary rat embryo (RE) cells. Our results show that in the presence of 10% calf serum, platelet-derived growth factor (PDGF), 1-30 ng/ml, has the strongest effect of all growth factors tested on AI growth. Type-beta transforming growth factor (TGF-beta), by itself, does not stimulate AI growth, and it inhibits the PDGF-induced colony formation in a dose-dependent manner (ED50 approximately 0.03 ng/ml). Qualitatively similar responses are obtained by using an established line of fibroblasts, NIH 3T3 cells; the principal difference between the response of the primary cells and the established cell line is in colony-forming efficiency in soft agar culture (15% and 90%, respectively, for growth of colonies greater than 1,500 micron2 diameter in the presence of 10 ng/ml PDGF). Since AI growth has been shown to correlate well with tumorigenicity in vivo, our results suggest that the transforming potential of PDGF in an appropriate responsive cell can be controlled not only through its interaction with its own receptor, but also by the presence of inhibitory factors such as TGF-beta.read more
Citations
More filters
Journal ArticleDOI
The biology of platelet-derived growth factor
TL;DR: The biology of platelet derived growth factor, it will really give you the good idea to be successful.
Journal ArticleDOI
Some recent advances in the chemistry and biology of transforming growth factor-beta.
TL;DR: It is clear that there is no one principal action for TGF-beta; moreover, the almost universal cellular distribution of its receptor encompasses a very broad spectrum of target tissues.
Journal ArticleDOI
Transforming growth factor-beta: biological function and chemical structure
TL;DR: TGF-beta's marked ability to enhance formation of connective tissue in vivo suggests several therapeutic applications.
Journal ArticleDOI
Transforming Growth Factor-β
TL;DR: Transforming growth factor-β (TGF-β) as mentioned in this paper is the cytokine with the broadest range of activities in repair of injured tissue, based both on the variety of cell types that produce and/or respond to it and on the spectrum of its cellular responses.
Book ChapterDOI
The Transforming Growth Factor-βs
A. B. Roberts,M. B. Sporn +1 more
TL;DR: This chapter summary of current knowledge of the chemistry and complex biology of the growing family of TGF-βs suggests that it has a pivotal control function in many physiological and pathological processes.
References
More filters
Journal ArticleDOI
Tumorigenic conversion of primary embryo fibroblasts requires at least two cooperating oncogenes.
TL;DR: The embryo fibroblasts become tumorigenic if a second oncogene such as a viral or cellular myc gene or the gene for the polyoma large-T antigen is introduced together with the ras gene.
Journal ArticleDOI
Close similarity of epidermal growth factor receptor and v- erb-B oncogene protein sequences
Julian Downward,Yosef Yarden,E. Mayes,G. Scrace,Nick Totty,P. Stockwell,Axel Ullrich,Joseph Schlessinger,Mike Waterfield +8 more
TL;DR: Six peptides derived from the human epidermal growth factor receptor very closely matches a part of the deduced sequence of the v-erb-B transforming protein of avian erythroblastosis virus (AEV).
Journal ArticleDOI
Transforming growth factor-beta in human platelets. Identification of a major storage site, purification, and characterization.
TL;DR: The results show that platelets contain a type beta transforming growth factor, which is distinct from platelet-derived growth factor and elicits 50% of its maximal biological response at concentrations less than 5 x 10(-12) M.
Journal ArticleDOI
Platelet-derived growth factor is structurally related to the putative transforming protein p28sis of simian sarcoma virus.
Michael D. Waterfield,Geoffrey T. Scrace,Nigel Whittle,Paul Stroobant,Ann Johnsson,Åke Wasteson,Bengt Westermark,Carl-Henrik Heldin,Jung San Huang,Thomas F. Deuel +9 more
TL;DR: A partial amino acid sequence of human platelet-derived growth factor, the major mitogen in serum for cells of mesenchymal origin, shows virtual identity with the predicted sequence of p28sis, the putative transforming protein of simian sarcoma virus (SSV).
Journal ArticleDOI
Simian sarcoma virus onc gene, v-sis, is derived from the gene (or genes) encoding a platelet-derived growth factor
Russell F. Doolittle,Michael W. Hunkapiller,Leroy Hood,Sushilkumar G. Devare,Keith C. Robbins,Stuart A. Aaronson,Harry N. Antoniades +6 more
TL;DR: The demonstrating of extensive sequence similarity between the transforming protein derived from the simian sarcoma virus onc gene, v-sis, and a human platelet-derived growth factor shows that this protein could be a factor active transiently during normal cell growth.
Related Papers (5)
Transforming growth factor-beta stimulates the expression of fibronectin and collagen and their incorporation into the extracellular matrix.
Ronald A. Ignotz,Joan Massagué +1 more