Journal ArticleDOI
Antibodies against the chemically synthesized genome-linked protein of poliovirus react with native virus-specific proteins.
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TLDR
The genome-linked protein (VPg) of poliovirus has been chemically synthesized, coupled to bovine serum albumin carrier and injected into rabbits and an antibody response was elicited not only by the full-length synthetic VPg peptide, but also by a synthetic 14-amino acid carboxy-terminal peptide.About:
This article is published in Cell.The article was published on 1982-02-01. It has received 100 citations till now. The article focuses on the topics: Blood serum & Bovine serum albumin.read more
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Book ChapterDOI
Turns in peptides and proteins.
TL;DR: The aim of this chapter is to examine structural and functional roles of turns in peptides and proteins.
Journal ArticleDOI
Three-Dimensional Structure of Poliovirus at 2.9 Å Resolution
TL;DR: The three-dimensional structure of poliovirus has been determined at 2.9 A resolution by x-ray crystallographic methods and the positions and interactions of the NH2- and COOH-terminal strands of the capsid proteins have important implications for virion assembly.
Journal ArticleDOI
Tapping the immunological repertoire to produce antibodies of predetermined specificity
TL;DR: This work has shown that chemically synthesized peptides representative of virtually any part of the surface of a protein can elicit antibodies reactive with the native molecule, and antibodies, useful in the study of changes in protein structure, can be generated.
Patent
Production of pluripotent granulocyte colony-stimulating factor.
TL;DR: Novel polypeptides possessing part or all of the primary structural conformation and one or more of the biological properties of a mammalian pluripotent granulocyte colony-stimulating factor (''hpG-CSF'') are characterized in preferred forms by being the product of procaryotic or eucaryotic host expression of an exogenous DNA sequence as mentioned in this paper.
Journal ArticleDOI
Structural factors that control conformational transitions and serotype specificity in type 3 poliovirus
TL;DR: The three‐dimensional structure of the Sabin strain of type 3 poliovirus has been determined and several newly identified components of the structure participate in assembly‐dependent interactions which are relevant to the biologically important processes of viral assembly and uncoating.
References
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Journal ArticleDOI
The preparation of 131i-labelled human growth hormone of high specific radioactivity
TL;DR: The loss of immunological reactivity at high specific radioactivities or at high levels of chemical substitution with STAI/sup 127/!iodine is demonstrated.
Journal ArticleDOI
Preparation of Iodine-131 Labelled Human Growth Hormone of High Specific Activity
W M Hunter,F. C. Greenwood +1 more
TL;DR: Current procedures for the immunological assay of protein hormones in human plasma require the routine preparation of hormones labelled with iodine-131 of high specific activity, and this work demonstrates the importance of knowing the carrier and removal status of iodine.
Journal ArticleDOI
Solid Phase Peptide Synthesis
TL;DR: This work has shown that effective methods of peptide synthesis are crucial to progress in this area, because only by synthesis can adequate amounts of important peptides be made available for chemical, biologi...
Journal ArticleDOI
The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Application to the radioimmunoassay
A. E. Bolton,William M. Hunter +1 more
TL;DR: With some antisera the immunoreactivity of the antigen was diminished by the introduction of a single I atom into the tyrosyl groups, whereas antigen containing a single (125)I-labelled 3-(4-hydroxyphenyl)propionamide group showed the same immunore activity as the unmodified antigen.
Journal Article
Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.
TL;DR: Bacterial adsorbent not only had a distinct advantage in speed of antigen isolation, but analyses by polyacrylamide gel electrophoresis in SDS also revealed consistently higher antigen recoveries, lower levels of background radioactivity, and an absence of other cell components which may nonspecifically bind to and complicate analyses using conventional immune precipitates.