Journal ArticleDOI
Application of yeasts in gene expression studies: a comparison of Saccharomyces cerevisiae, Hansenula polymorpha and Kluyveromyces lactis -- a review.
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TLDR
The present status of the various yeast systems is discussed, with Kluyveromyces lactis, and the methylotrophs Hansenula polymorpha and Pichia pastoris having been proven to offer significant advantages over the traditional baker's yeast for the production of certain proteins.About:
This article is published in Gene.The article was published on 1997-01-01. It has received 219 citations till now. The article focuses on the topics: Kluyveromyces lactis & Pichia pastoris.read more
Citations
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Journal ArticleDOI
Recombinant protein expression in Pichia pastoris.
TL;DR: Major advances in the development of new strains and vectors, improved techniques, and the commercial availability of these tools coupled with a better understanding of the biology of Pichia species have led to this microbe’s value and power in commercial and research labs alike.
Journal ArticleDOI
The yeast Kluyveromyces marxianus and its biotechnological potential.
Gustavo Graciano Fonseca,Gustavo Graciano Fonseca,Elmar Heinzle,Christoph Wittmann,Andreas Karoly Gombert,Andreas Karoly Gombert +5 more
TL;DR: To increase knowledge on the biology of this species and to enable the potential applications to be converted into industrial practice, a more systematic approach, including the careful choice of (a) reference strain(s) by the scientific community, would certainly be of great value.
Journal ArticleDOI
Heterologous protein expression and secretion in the non-conventional yeast Yarrowia lipolytica: a review.
TL;DR: This review presents the main characteristics of Y. lipolytica, and the genetic and molecular tools available in this yeast, and a particular emphasis is given to newly developed tools such as efficient promoters, a non-homologous integration method, and an amplification system using defective selection markers.
Journal ArticleDOI
Recombinant expression systems in the pharmaceutical industry.
TL;DR: To fully exploit the secretory capacity of fungal species, a deeper understanding of their posttranslational modification physiology will be necessary to steer the degree and pattern of glycosylation, which influences both folding and secretion efficiency.
Book ChapterDOI
Recombinant Protein Production in Yeasts
TL;DR: Advantages and limitations of the main and most promising yeast hosts, including Saccharomyces cerevisiae, Pichia pastoris, and Hansenula polymorpha as those presently used in large scale production of heterologous proteins are summarized.
References
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Journal ArticleDOI
Life with 6000 Genes
André Goffeau,Bart Barrell,Howard Bussey,Ronald W. Davis,Bernard Dujon,Horst Feldmann,Francis Galibert,J D Hoheisel,Claude Jacq,Mark Johnston,Edward J. Louis,Hans-Werner Mewes,Yasufumi Murakami,Peter Philippsen,Hervé Tettelin,Stephen G. Oliver +15 more
TL;DR: The genome of the yeast Saccharomyces cerevisiae has been completely sequenced through a worldwide collaboration and provides information about the higher order organization of yeast's 16 chromosomes and allows some insight into their evolutionary history.
Book
Molecular Biology of the Gene
TL;DR: The long-awaited Fifth Edition of James D. Watson's classic text, Molecular Biology of the Gene, has been thoroughly revised and is published to coincide with the 50th anniversary of Watson and Crick's paper on the structure of the DNA double-helix as discussed by the authors.
Journal ArticleDOI
Recent advances in the expression of foreign genes in Pichia pastoris
TL;DR: The Pichia pastoris heterologous gene expression system has been utilized to produce attractive levels of a variety of intracellular and extracellular proteins of interest and improvements in understanding and application have improved its utility even further.