Artificial Biocatalytic Linear Cascades for Preparation of Organic Molecules
TLDR
The review introduces a systematic classification of the cascades according to the number of enzymes in the linear sequence and differentiates between cascades involving exclusively enzymes and combinations of enzymes with non-natural catalysts or chemical steps.Abstract:
The review compiles artificial cascades involving enzymes with a focus on the last 10 years. A cascade is defined as the combination of at least two reaction steps in a single reaction vessel without isolation of the intermediates, whereby at least one step is catalyzed by an enzyme. Additionally, cascades performed in vivo and in vitro are discussed separately, whereby in vivo cascades are defined here as cascades relying on cofactor recycling by the metabolism or on a metabolite from the living organism. The review introduces a systematic classification of the cascades according to the number of enzymes in the linear sequence and differentiates between cascades involving exclusively enzymes and combinations of enzymes with non-natural catalysts or chemical steps. Since the number of examples involving two enzymes is predominant, the two enzyme cascades are further subdivided according to the number, order, and type of redox steps. Furthermore, this classification differentiates between cascades where al...read more
Citations
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Biocatalysis: Enzymatic Synthesis for Industrial Applications
TL;DR: A review of biocatalysis with a special focus on scalable chemical production using enzymes discusses the opportunities and limitations of enzymatic syntheses using distinct examples and provides an outlook on emerging enzyme classes.
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Design of an in vitro biocatalytic cascade for the manufacture of islatravir
Mark A. Huffman,Anna Fryszkowska,Oscar Alvizo,Margie Borra-Garske,Kevin R. Campos,Canada Keith A,Paul N. Devine,Da Duan,Jacob Forstater,Shane T. Grosser,Holst M. Halsey,Gregory Hughes,Junyong Jo,Leo A. Joyce,Joshua N. Kolev,Jack Liang,Kevin M. Maloney,Benjamin F. Mann,Nicholas M. Marshall,Mark McLaughlin,Jeffrey C. Moore,Grant S. Murphy,Christopher C. Nawrat,Jovana Nazor,Scott J. Novick,Niki R. Patel,Agustina Rodriguez-Granillo,Sandra A. Robaire,Edward C. Sherer,Matthew D. Truppo,Aaron M. Whittaker,Deeptak Verma,Li Xiao,Yingju Xu,Hao Yang +34 more
TL;DR: An in vitro biocatalytic cascade synthesis of the investigational HIV treatment islatravir is reported, requiring fewer than half the number of steps of the previously reported routes.
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Biocatalytic Oxidation Reactions: A Chemist's Perspective
JiaJia Dong,Elena Fernández-Fueyo,Frank Hollmann,Caroline E. Paul,Milja Pesic,Sandy Schmidt,Yonghua Wang,Sabry H. H. Younes,Wuyuan Zhang +8 more
TL;DR: The most important recent developments in the field of biocatalytic oxidation chemistry are critically summarised and the most pressing bottlenecks as well as promising solutions are identified.
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Biomimetic enzyme cascade reaction system in microfluidic electrospray microcapsules.
TL;DR: A novel multienzyme system based on hollow hydrogel microcapsules with flexible enzymatic inverse opal particles that could act as a cascade biocatalyst and reduce alcohol levels in media, providing an alternative antidote and prophylactic for alcohol intoxication.
Journal ArticleDOI
Extending the application of biocatalysis to meet the challenges of drug development
Paul N. Devine,Roger M. Howard,Rajesh Kumar,Matthew P. Thompson,Matthew D. Truppo,Matthew D. Truppo,Nicholas J. Turner +6 more
TL;DR: Significant advances in enzyme discovery, high-throughput screening and protein engineering have substantially expanded the available biocatalysts, and consequently, many more synthetic transformations are now possible, leading to greater predictability and confidence when scaling up these processes.
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