Journal ArticleDOI
Au Nanoparticle-Based Surface Energy Transfer Probe for Conformational Changes of BSA Protein
Reads0
Chats0
TLDR
Results indicate that Au nanoparticle quenches BSA fluorescence mainly through a static quenching mechanism, and analysis suggests that binding constant and bound/unbound ratio varies with changing the conformation of protein.Abstract:
In the present study, Au nanoparticle based surface energy transfer (SET) has been used to measure conformational changes in proteins. A significant photoluminescence (PL) quenching (91−97%) of tryptophan intensities of bovine serum albumin (BSA) protein is observed in the presence of Au nanoparticles, and the measured distances (r) between the donor (tryptophan) and the acceptor (Au nanoparticle) are 27.0, 22.9, and 25.7 A for E, N, and B forms of BSA protein, respectively. Results indicate that Au nanoparticle quenches BSA fluorescence mainly through a static quenching mechanism. Analysis suggests that binding constant and bound/unbound ratio varies with changing the conformation of protein. The PL quenching of dye varies from 47.2 to 86.6% with changing the conformation of protein without changing the radiative rate of dye. The measured distances (d) between the donor (dye) and the acceptor (Au nanoparticle) are 116.5, 76.1, and 86.4 A for E, N, and B forms of BSA protein, respectively, using the effic...read more
Citations
More filters
Journal ArticleDOI
Analyzing nanomaterial bioconjugates: a review of current and emerging purification and characterization techniques.
Journal ArticleDOI
Gold nanoparticle probes for the detection of mercury, lead and copper ions.
TL;DR: The roles of several factors affecting the selectivity and sensitivity of the nanosensors toward the analytes are discussed: the size of the Au nanomaterial, the length and sequence of the DNA or the nature of the thiol, the surface density of the recognition ligand, the ionic strength and pH of the buffer solution.
Journal ArticleDOI
Nanoparticle-protein interactions: a thermodynamic and kinetic study of the adsorption of bovine serum albumin to gold nanoparticle surfaces.
Stefano P. Boulos,Tyler A. Davis,Jie An Yang,Samuel E. Lohse,Alaaldin M. Alkilany,Lisa A. Holland,Catherine J. Murphy +6 more
TL;DR: A library of spherical and rod-shaped gold nanoparticles (GNPs) was used to evaluate the process of protein adsorption to their surfaces, and equilibrium binding constant determinations indicated that BSA has a comparable binding affinity to all of the GNPs tested, regardless of surface charge.
Journal ArticleDOI
Involvement of the LSPR spectral overlap for energy transfer between a dye and Au nanoparticle.
TL;DR: The results show that the LSPR plays a very important role in the observed quenching of excited-state donors at the surface of the nanometal, and the correlation to the NSET model allows a compilation of the necessary biophysical constants for application within the toolbox of biophysics.
Journal ArticleDOI
Systematic investigation on the interaction of bovine serum albumin with ZnO nanoparticles using fluorescence spectroscopy
A. Bhogale,Nainesh Patel,Padmanabh S. Sarpotdar,Jessy Mariam,P. M. Dongre,Antonio Miotello,D.C. Kothari +6 more
TL;DR: The synchronous fluorescence spectra reveal that the microenvironment close to both the tyrosine and tryptophan residues of BSA is perturbed and that the hydrophobicity of both the residues is increased in the presence of ZnO NPs.
References
More filters
Book
Principles of fluorescence spectroscopy
TL;DR: This book describes the fundamental aspects of fluorescence, the biochemical applications of this methodology, and the instrumentation used in fluorescence spectroscopy.
Book ChapterDOI
Structure of serum albumin.
Daniel C. Carter,Joseph X. Ho +1 more
TL;DR: This chapter provides an insight of the findings of past significant papers with the current knowledge of the recently determined high resolution X-ray structure of serum albumin and suggests that AFP may have a higher affinity for some unknown ligands important for fetal development.
Journal ArticleDOI
10th Spiers Memorial Lecture. Transfer mechanisms of electronic excitation
Journal ArticleDOI
Self-assembled nanoscale biosensors based on quantum dot FRET donors
Igor L. Medintz,Aaron R. Clapp,Hedi Mattoussi,Ellen R. Goldman,Brent Fisher,J. Matthew Mauro,J. Matthew Mauro +6 more
TL;DR: The design, formation and testing of QD–protein assemblies that function as chemical sensors that overcomes inherent QD donor–acceptor distance limitations are reported.
Journal ArticleDOI
Fluorescence quenching of dye molecules near gold nanoparticles: radiative and nonradiative effects.
E. Dulkeith,A.C. Morteani,T. Niedereichholz,Thomas A. Klar,Jochen Feldmann,Stefano Levi,F.C.J.M. van Veggel,David N. Reinhoudt,Martin Möller,David I. Gittins +9 more
TL;DR: The radiative and nonradiative decay rates of lissamine dye molecules, chemically attached to differently sized gold nanoparticles, are investigated by means of time-resolved fluorescence experiments and theoretical results derived from the Gersten-Nitzan model are compared.