Journal ArticleDOI
Biofilm formation and antibiotic resistance in Klebsiella pneumoniae urinary strains.
Claudia Vuotto,Francesca Longo,Chiara Pascolini,Gianfranco Donelli,Maria Pia Balice,Maria Francesca Libori,Valentina Tiracchia,Antonino Salvia,Pietro E. Varaldo +8 more
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TLDR
The aim of this study was to analyse some virulence factors of K. pneumoniae isolates, and to evaluate possible correlations between their antibiotic resistance profile and ability to form biofilm.Abstract:
Aims
Multi-drug resistant Klebsiella pneumoniae has become a relevant healthcare-associated pathogen. Capsule, type 1 and 3 fimbriae (mrkA gene), type 2 quorum-sensing system (luxS), synthesis of D-galactan I (wbbM), LPS transport (wzm) and poly-beta-1,6-N-acetyl-D-glucosamine (pgaA) seem involved in K. pneumoniae biofilm. Non-enzymatic antibiotic resistance is related to non-expression or mutation of porins (OmpK35 and OmpK36), and efflux pump (acrB) over-expression. The aim of this study was to analyse some virulence factors of K. pneumoniae isolates, and to evaluate possible correlations between their antibiotic resistance profile and ability to form biofilm.
Methods and Results
Quantitative biofilm production assay, congo red agar test and string test were performed on 120 isolates clustered in 56 extensively drug resistant (XDR, 40 MDR and 24 susceptible (S)). Nine representative strains were analyzed by real-time RT-PCR for the expression of antibiotic resistance (OmpK35, OmpK36, acrB) and biofilm production genes (mrkA, luxS,, pga, wbbM, wzm) during planktonic and sessile growth. XDR isolates showed a higher ability to form biofilm (91.07%) and to produce polysaccharides (78.57%) when compared to MDR and S strains. In biofilm-growing XDR strains, 7 out of 8 genes were upregulated, with the only exception of OmpK36.
Conclusions
XDR strains exhibited phenotypic and genotypic features supporting a significant growth as biofilm.
Significance and Impact of Study
this study produces new findings that highlight a positive correlation between antibiotic resistance profile and biofilm-forming ability in XDR K. pneumoniae strains. These new evidences might contribute to the progress in selection of therapeutic treatments of infections caused by Klebsiella pneumoniae resistant also to the “last line of defense” antibiotics, i.e. carbapenems.
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Citations
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Emerging Strategies to Combat ESKAPE Pathogens in the Era of Antimicrobial Resistance: A Review.
TL;DR: The objective of this review is to describe the alternative therapies reported to treat ESKAPE infections, their advantages and limitations, potential application in vivo, and status in clinical trials.
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Interference in Bacterial Quorum Sensing: A Biopharmaceutical Perspective.
TL;DR: The efficacy of QSIs and QQ enzymes has been demonstrated in various animal models and are now considered in the development of new medical devices against bacterial infections, including dressings, and catheters for enlarging the therapeutic arsenal against bacteria.
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Biofilms as Promoters of Bacterial Antibiotic Resistance and Tolerance.
TL;DR: A deep understanding of the mechanisms by which biofilms cause tolerance/resistance to antibiotics helps to develop novel strategies to fight these infections as discussed by the authors, which is a global threat for human and animal health.
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Synthetic small molecules as anti-biofilm agents in the struggle against antibiotic resistance.
Barbara Parrino,Domenico Schillaci,Ilaria Carnevale,Elisa Giovannetti,Elisa Giovannetti,Patrizia Diana,Girolamo Cirrincione,Stella Cascioferro +7 more
TL;DR: The most relevant literature of the last decade is selected, focusing on the development of synthetic small molecules able to prevent bacterial biofilm formation or to eradicate pre-existing biofilms of clinically relevant Gram-positive and Gram-negative pathogens.
Journal ArticleDOI
Biofilm formation and antibiotic resistance of Klebsiella pneumoniae isolated from clinical samples in a tertiary care hospital, Klaten, Indonesia
Hera Nirwati,Kian Sinanjung,Fahrina Fahrunissa,Fernando Wijaya,Sarastia Napitupulu,Vania P. Hati,Mohamad S. Hakim,Andreanita Meliala,Abu Tholib Aman,Titik Nuryastuti +9 more
TL;DR: Most of the K. pneumoniae isolates demonstrated resistance to a wide range of antibiotics and are biofilm producers.
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