Journal ArticleDOI
Bioprinting of Stem Cells: Interplay of Bioprinting Process, Bioinks, and Stem Cell Properties.
TLDR
This work reviews the recent development of stem cell bioprinting technology and concludes on the major factors regulating stem cell viability, proliferation, differentiation, and function from the aspects of the choice of biopprinting techniques, the modulation of biOPrinting parameters, and the regulation of the stem cell niche in the whole lifespan of biopsying practices.Abstract:
Combining the advantages of 3D bioprinting technology and biological characteristics of stem cells, bioprinting of stem cells is recognized as a novel technology with broad applications in biological study, drug testing, tissue engineering, regenerative medicine, etc However, the biological performance and functional reconstruction of stem cells are greatly influenced by both the bioprinting process and post-bioprinting culture conditions, which are critical factors to consider for further applications Here we review the recent development of stem cell bioprinting technology and conclude on the major factors regulating stem cell viability, proliferation, differentiation, and function from the aspects of the choice of bioprinting techniques, the modulation of bioprinting parameters, and the regulation of the stem cell niche in the whole lifespan of bioprinting practices We aim to provide a comprehensive consideration and guidance regarding the bioprinting of stem cells for optimization of this promisingread more
Citations
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Organ Printing: Tissue Spheroids as Building Blocks
Vladimir Mironov,Jing Zhang,Carmine Gentile,K Brakke,Thomas C. Trusk,Karoly Jakab,Gabor Forgacs,Vladimirs Kasjanovs,Richard P. Visconti,Roger R. Markwald +9 more
TL;DR: Organ printing can be defined as layer-by-layer additive robotic biofabrication of three-dimensional functional living macrotissues and organ constructs using tissue spheroids as building blocks.
Journal ArticleDOI
A Review on the Adaption of Alginate-Gelatin Hydrogels for 3D Cultures and Bioprinting.
Magdalena Beata Łabowska,Karolina Cierluk,Agnieszka M. Jankowska,Julita Kulbacka,Jerzy Detyna,Izabela Michalak +5 more
TL;DR: An overview of the materials used for 3D cell cultures, which are mainly alginate-gelatin hydrogels, including their properties and potential applications, can be found in this paper.
Journal ArticleDOI
Synthetic Biodegradable Aliphatic Polyester Nanocomposites Reinforced with Nanohydroxyapatite and/or Graphene Oxide for Bone Tissue Engineering Applications.
TL;DR: Review updates on the current development of bionanocomposites with polymeric matrices consisting of synthetic biodegradable aliphatic polyesters reinforced with nanohydroxyaptite (nHA) and/or graphene oxide (GO) nanofillers for bone tissue engineering applications are provided.
Journal ArticleDOI
Peptide Chitosan/Dextran Core/Shell Vascularized 3D Constructs for Wound Healing.
TL;DR: C/s 3D bioprinted, peptide-CD/GelMA constructs provided the appropriate microenvironment for in vitro stem and endothelial cell viability, delivery, and differentiation and are foresee as representing a fundamental step toward engineering larger scale regenerative, pre-vascularized tissues.
Journal ArticleDOI
Fast Stereolithography Printing of Large-Scale Biocompatible Hydrogel Models.
Nanditha Anandakrishnan,Hang Ye,Zipeng Guo,Zhaowei Chen,Kyle I Mentkowski,Kyle I Mentkowski,Jennifer K Lang,Jennifer K Lang,Nika Rajabian,Stelios T. Andreadis,Zhen Ma,Joseph A. Spernyak,Jonathan F. Lovell,Depeng Wang,Jun Xia,Chi Zhou,Ruogang Zhao +16 more
TL;DR: In this article, a fast hydrogel stereolithography printing (FLOAT) method is presented that allows the creation of a centimeter-sized, multiscale solid hydrogels model within minutes.
References
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Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Journal ArticleDOI
Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
Journal ArticleDOI
Stem cells, cancer, and cancer stem cells
TL;DR: Stem cell biology has come of age: Unequivocal proof that stem cells exist in the haematopoietic system has given way to the prospective isolation of several tissue-specific stem and progenitor cells, the initial delineation of their properties and expressed genetic programmes, and the beginnings of their utility in regenerative medicine.