Journal ArticleDOI
3D bioprinting of collagen to rebuild components of the human heart
Andrew Lee,Andrew Hudson,Daniel J. Shiwarski,Joshua W. Tashman,Thomas J. Hinton,Saigopalakrishna S. Yerneni,Jacqueline M. Bliley,Phil G. Campbell,Adam W. Feinberg +8 more
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TLDR
3D-bioprinted hearts accurately reproduce patient-specific anatomical structure as determined by micro–computed tomography and showed synchronized contractions, directional action potential propagation, and wall thickening up to 14% during peak systole.Abstract:
Collagen is the primary component of the extracellular matrix in the human body. It has proved challenging to fabricate collagen scaffolds capable of replicating the structure and function of tissues and organs. We present a method to 3D-bioprint collagen using freeform reversible embedding of suspended hydrogels (FRESH) to engineer components of the human heart at various scales, from capillaries to the full organ. Control of pH-driven gelation provides 20-micrometer filament resolution, a porous microstructure that enables rapid cellular infiltration and microvascularization, and mechanical strength for fabrication and perfusion of multiscale vasculature and tri-leaflet valves. We found that FRESH 3D-bioprinted hearts accurately reproduce patient-specific anatomical structure as determined by micro-computed tomography. Cardiac ventricles printed with human cardiomyocytes showed synchronized contractions, directional action potential propagation, and wall thickening up to 14% during peak systole.read more
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3D printing of hydrogels: Rational design strategies and emerging biomedical applications
TL;DR: A review of hydrogel-based biomaterial inks and bioinks for 3D printing can be found in this paper, where the authors provide a comprehensive overview and discussion of the tailorability of material, mechanical, physical, chemical and biological properties.
Journal ArticleDOI
Printability and Shape Fidelity of Bioinks in 3D Bioprinting.
Andrea Schwab,Riccardo Levato,Matteo D'Este,Susanna Piluso,Susanna Piluso,David Eglin,Jos Malda +6 more
TL;DR: The physicochemical parameters influencing shape fidelity are discussed, together with their importance in establishing new models, predictive tools and printing methods that are deemed instrumental for the design of next-generation bioinks, and for reproducible comparison of their structural performance.
Journal ArticleDOI
Engineered biomaterials for in situ tissue regeneration
TL;DR: Two biomaterials approaches to control the regenerative capacity of the body for tissue-specific regeneration by modulating the extracellular microenvironment or driving cellular reprogramming are outlined.
Journal ArticleDOI
Soft Materials by Design: Unconventional Polymer Networks Give Extreme Properties.
TL;DR: In this paper, a review aimed at synergistically reporting: (i) general design principles for hydrogels to achieve extreme mechanical and physical properties, (ii) implementation strategies for the design principles using unconventional polymer networks, and (iii) future directions for the orthogonal design of hydrogel to achieve multiple combined mechanical, physical, chemical, and biological properties.
References
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Journal ArticleDOI
The extracellular matrix at a glance
TL;DR: The extracellular matrix is the non-cellular component present within all tissues and organs, and provides not only essential physical scaffolding for the cellular constituents but also initiates crucial biochemical and biomechanical cues that are required for tissue development.
Journal ArticleDOI
VEGF receptor signalling - in control of vascular function.
TL;DR: Recent insights have shed light onto VEGFR signal transduction and the interplay between different V EGFRs and VEGF co-receptors in development, adult physiology and disease.
Journal ArticleDOI
Basic and Therapeutic Aspects of Angiogenesis
TL;DR: The emerging principles of vascular growth provide exciting new perspectives, the translation of which might overcome the current limitations of pro- and antiangiogenic medicine.
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A 3D bioprinting system to produce human-scale tissue constructs with structural integrity
TL;DR: An integrated tissue–organ printer (ITOP) that can fabricate stable, human-scale tissue constructs of any shape is presented and the incorporation of microchannels into the tissue constructs facilitates diffusion of nutrients to printed cells, thereby overcoming the diffusion limit of 100–200 μm for cell survival in engineered tissues.
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Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues
Jordan S. Miller,Kelly R. Stevens,Michael T. Yang,Brendon M. Baker,Duc-Huy T. Nguyen,Daniel M. Cohen,Esteban Toro,Alice A. Chen,Peter A. Galie,Xiang-Qing Yu,Ritika Chaturvedi,Sangeeta N. Bhatia,Sangeeta N. Bhatia,Christopher S. Chen +13 more
TL;DR: 3D printed rigid filament networks of carbohydrate glass are used as a cytocompatible sacrificial template in engineered tissues containing living cells to generate cylindrical networks which could be lined with endothelial cells and perfused with blood under high-pressure pulsatile flow.