Bioreactor design for perfusion-based, highly-vascularized organ regeneration.
TLDR
This review focuses on the state-of-art in bioreactor development for perfusion-based bioartificial organs and highlights specific design components in need of further refinement.Abstract:
Bioartificial or laboratory-grown organs is a growing field centered on developing replacement organs and tissues to restore body function and providing a potential solution to the shortage of donor organs for transplantation. With the entry of engineered planar tissues, such as bladder and trachea, into clinical studies, an increasing focus is being given to designing complex, three-dimensional solid organs. As tissues become larger, thicker and more complex, the vascular network becomes crucial for supplying nutrients and maintaining viability and growth of the neo-organ. Perfusion decellularization, the process of removing cells from an entire organ, leaves the matrix of the vascular network intact. Organ engineering requires a delicate process of decellularization, sterilization, reseeding with appropriate cells, and organ maturation and stimulation to ensure optimal development. The design of bioreactors to facilitate this sequence of events has been refined to the extent that some bioartificial organs grown in these systems have been transplanted into recipient animals with sustained, though limited, function. This review focuses on the state-of-art in bioreactor development for perfusion-based bioartificial organs and highlights specific design components in need of further refinement.read more
Citations
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Tissue engineering by decellularization and 3D bioprinting
Elena Garreta,Roger Oria,Carolina Tarantino,Mateu Pla-Roca,Patrícia Rezende do Prado,Francisco Fernández-Avilés,Josep M. Campistol,Josep Samitier,Nuria Montserrat +8 more
TL;DR: Recent progress in decellularization/re cellularization and 3D bioprinting technologies are reviewed, aiming to fabricate autologous tissue grafts and organs with an impact in regenerative medicine.
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Optimization and Critical Evaluation of Decellularization Strategies to Develop Renal Extracellular Matrix Scaffolds as Biological Templates for Organ Engineering and Transplantation
Mireia Caralt,Joseph S. Uzarski,Stanca Iacob,Kyle P. Obergfell,Natasha Berg,Brent M. Bijonowski,Kathryn M. Kiefer,Heather H. Ward,Angela Wandinger-Ness,William M. Miller,Zheng Zhang,Michael Abecassis,Jason A. Wertheim +12 more
TL;DR: Triton/SDS‐decellularized scaffolds maintained 3 h of leak‐free blood flow in a rodent transplantation model and supported repopulation with human iPSC‐derived endothelial cells and tubular epithelial cells ex vivo.
Journal ArticleDOI
A Nonhuman Primate Model of Lung Regeneration: Detergent-Mediated Decellularization and Initial In Vitro Recellularization with Mesenchymal Stem Cells
Ryan W. Bonvillain,Svitlana Danchuk,Deborah E. Sullivan,Aline M. Betancourt,Julie A. Semon,Michelle E. Eagle,Jacques P. Mayeux,Ashley N. Gregory,Guangdi Wang,Ian K. Townley,Zachary D. Borg,Daniel J. Weiss,Bruce A. Bunnell +12 more
TL;DR: Nonhuman primates (NHP) provide a highly relevant preclinical model with which to assess the feasibility of recellularized lung scaffolds for human lung transplantation and both bone marrow- and adipose-derived mesenchymal stem cells attach to the decellularized lung matrix and can be maintained within this environment in vitro, suggesting that these cells may be promising candidates and useful tools for lung regeneration.
Journal ArticleDOI
The multiple functions of melatonin in regenerative medicine
Maryam Majidinia,Russel J. Reiter,Seyed Kazem Shakouri,Iraj Mohebbi,Mojgan Rastegar,Mojtaba Kaviani,Saber Ghazizadeh Darband,Rana Jahanban-Esfahlan,Seyed Mohammad Nabavi,Bahman Yousefi +9 more
TL;DR: The recent findings on the molecular involvement of melatonin in the regeneration of various tissues including the nervous system, liver, bone, kidney, bladder, skin, and muscle are discussed.
Journal ArticleDOI
Essential design considerations for the resazurin reduction assay to noninvasively quantify cell expansion within perfused extracellular matrix scaffolds.
TL;DR: While the resazurin reduction assay provides a powerful, noninvasive readout of metrics enumerating cellularity and growth within ECM scaffolds, assay conditions may strongly influence its applicability for accurate quantification of cell number.
References
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Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart
Harald C. Ott,Thomas S Matthiesen,Saik Kia Goh,Lauren D. Black,Stefan M. Kren,Theoden I. Netoff,Doris A. Taylor +6 more
TL;DR: Eight constructs decellularized hearts by coronary perfusion with detergents, preserved the underlying extracellular matrix, and produced an acellular, perfusable vascular architecture, competent a cellular valves and intact chamber geometry that could generate pump function in a modified working heart preparation.
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Oxygen in Stem Cell Biology: A Critical Component of the Stem Cell Niche
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Journal ArticleDOI
Organ reengineering through development of a transplantable recellularized liver graft using decellularized liver matrix
Basak E. Uygun,Alejandro Soto-Gutierrez,Alejandro Soto-Gutierrez,Hiroshi Yagi,Hiroshi Yagi,Maria Louisa Izamis,Maria Angela Guzzardi,Maria Angela Guzzardi,Carley Shulman,Jack M. Milwid,Naoya Kobayashi,Arno W. Tilles,Francois Berthiaume,Francois Berthiaume,Martin Hertl,Yaakov Nahmias,Yaakov Nahmias,Martin L. Yarmush,Martin L. Yarmush,Korkut Uygun +19 more
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Journal ArticleDOI
Regeneration and orthotopic transplantation of a bioartificial lung
Harald C. Ott,Ben Clippinger,Claudius Conrad,Christian Schuetz,Irina Pomerantseva,Laertis Ikonomou,Darrell N. Kotton,Joseph P. Vacanti +7 more
TL;DR: Creation of a bioartificial lung requires engineering of viable lung architecture enabling ventilation, perfusion and gas exchange, and regenerated lungs into orthotopic position showed in vivo function.
Journal ArticleDOI
Whole-Organ Tissue Engineering: Decellularization and Recellularization of Three-Dimensional Matrix Scaffolds
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