Journal ArticleDOI
Regeneration and orthotopic transplantation of a bioartificial lung
Harald C. Ott,Ben Clippinger,Claudius Conrad,Christian Schuetz,Irina Pomerantseva,Laertis Ikonomou,Darrell N. Kotton,Joseph P. Vacanti +7 more
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TLDR
Creation of a bioartificial lung requires engineering of viable lung architecture enabling ventilation, perfusion and gas exchange, and regenerated lungs into orthotopic position showed in vivo function.Abstract:
About 2,000 patients now await a donor lung in the United States. Worldwide, 50 million individuals are living with endstage lung disease. Creation of a bioartificial lung requires engineering of viable lung architecture enabling ventilation, perfusion and gas exchange. We decellularized lungs by detergent perfusion and yielded scaffolds with acellular vasculature, airways and alveoli. To regenerate gas exchange tissue, we seeded scaffolds with epithelial and endothelial cells. To establish function, we perfused and ventilated cellseeded constructs in a bioreactor simulating the physiologic environment of developing lung. By day 5, constructs could be perfused with blood and ventilated using physiologic pressures, and they generated gas exchange comparable to that of isolated native lungs. To show in vivo function, we transplanted regenerated lungs into orthotopic position. After transplantation, constructs were perfused by the recipient’s circulation and ventilated by means of the recipient’s airway and respiratory muscles, and they provided gas exchange in vivo for up to 6 h after extubation.read more
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An overview of tissue and whole organ decellularization processes.
TL;DR: Tissue decellularization with preservation of ECM integrity and bioactivity can be optimized by making educated decisions regarding the agents and techniques utilized during processing.
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The acute respiratory distress syndrome.
TL;DR: Progress has been made in understanding the mechanisms responsible for the pathogenesis and the resolution of lung injury, including the contribution of environmental and genetic factors, and on developing novel therapeutics that can facilitate and enhance lung repair.
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Printing three-dimensional tissue analogues with decellularized extracellular matrix bioink
Falguni Pati,Jinah Jang,Dong-Heon Ha,Sung Won Kim,Jong Won Rhie,Jin-Hyung Shim,Deok Ho Kim,Dong-Woo Cho +7 more
TL;DR: The versatility and flexibility of the developed bioprinting process using tissue-specific dECM bioinks, including adipose, cartilage and heart tissues, capable of providing crucial cues for cells engraftment, survival and long-term function are shown.
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Whole-Organ Tissue Engineering: Decellularization and Recellularization of Three-Dimensional Matrix Scaffolds
TL;DR: In this paper, the fundamental concepts of whole-organ engineering, including characterization of the extracellular matrix as a scaffold, methods for decellularization of vascular organs, potential cells to reseed such an acellular, naturally occurring three-dimensional biologic scaffold material that can then be seeded with selected cell populations.
Whole-Organ Tissue Engineering: Decellularization and Recellularization of Three-Dimensional
TL;DR: The fundamental concepts of whole-organ engineering, including characterization of the extracellular matrix as a scaffold, methods for decellularization of vascular organs, potential cells to reseed such a scaffolds, techniques for the recellularization process and important aspects regarding bioreactor design to support this approach are described.
References
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Journal ArticleDOI
Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart
Harald C. Ott,Thomas S Matthiesen,Saik Kia Goh,Lauren D. Black,Stefan M. Kren,Theoden I. Netoff,Doris A. Taylor +6 more
TL;DR: Eight constructs decellularized hearts by coronary perfusion with detergents, preserved the underlying extracellular matrix, and produced an acellular, perfusable vascular architecture, competent a cellular valves and intact chamber geometry that could generate pump function in a modified working heart preparation.
Journal ArticleDOI
Identification of Bronchioalveolar Stem Cells in Normal Lung and Lung Cancer
Carla F. Kim,Erica L. Jackson,Amber Woolfenden,Sharon Lawrence,Imran Babar,Sinae Vogel,Denise Crowley,Roderick T. Bronson,Tyler Jacks +8 more
TL;DR: Although bronchiolar cells and alveolar cells are proposed to be the precursor cells of adenocarcinoma, this work points to bronchioalveolar stem cells as the putative cells of origin for this subtype of lung cancer.
Journal ArticleDOI
Generation of human induced pluripotent stem cells by direct delivery of reprogramming proteins.
Dohoon Kim,Chun-Hyung Kim,Jung-Il Moon,Young-Gie Chung,Mi-Yoon Chang,Baek-Soo Han,Sanghyeok Ko,Eungi Yang,Kwang Yul Cha,Robert Lanza,Kwang Soo Kim +10 more
TL;DR: Document S1.
Journal ArticleDOI
Clinical transplantation of a tissue-engineered airway
Paolo Macchiarini,Philipp Jungebluth,Tetsuhiko Go,M. Adelaide Asnaghi,Louisa E.N. Rees,Tristan A Cogan,A. Dodson,Jaume Martorell,Silvia Bellini,Pier Paolo Parnigotto,Sally C. Dickinson,Anthony P. Hollander,Sara Mantero,Maria Teresa Conconi,Martin A. Birchall +14 more
TL;DR: The results show that a cellular, tissue-engineered airway with mechanical properties that allow normal functioning, and which is free from the risks of rejection, is produced, suggesting that autologous cells combined with appropriate biomaterials might provide successful treatment for patients with serious clinical disorders.
Journal ArticleDOI
Organ reengineering through development of a transplantable recellularized liver graft using decellularized liver matrix
Basak E. Uygun,Alejandro Soto-Gutierrez,Alejandro Soto-Gutierrez,Hiroshi Yagi,Hiroshi Yagi,Maria Louisa Izamis,Maria Angela Guzzardi,Maria Angela Guzzardi,Carley Shulman,Jack M. Milwid,Naoya Kobayashi,Arno W. Tilles,Francois Berthiaume,Francois Berthiaume,Martin Hertl,Yaakov Nahmias,Yaakov Nahmias,Martin L. Yarmush,Martin L. Yarmush,Korkut Uygun +19 more
TL;DR: A novel approach to generate transplantable liver grafts using decellularized liver matrix that preserves the structural and functional characteristics of the native microvascular network, allowing efficient recellularization of the liver matrix with adult hepatocytes and subsequent perfusion for in vitro culture.
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