Journal ArticleDOI
Biosynthesis of lipids and organic acids by Yarrowia lipolytica strains cultivated on glucose
Seraphim Papanikolaou,Afroditi Chatzifragkou,Stylianos Fakas,Stylianos Fakas,Maria Galiotou-Panayotou,Michael Komaitis,Jean-Marc Nicaud,George Aggelis +7 more
TLDR
In several of the strains cultured in media containing a low glucose concentration, unicellular morphology was observed, while at high glucose concentrations mycelia were predominant, indicating lipid degradation even for the genetically modified strains.Abstract:
The biochemical behavior of wild-type or genetically modified (presenting decreased expression of intracellular acyl-CoA oxidases) Yarrowia lipolytica strains cultivated on commercial glucose was studied. Flask nitrogen-limited cultures were performed favoring the production of organic acids (and potentially the accumulation of lipid). Nitrogen depletion induced secretion of citric acid, while intracellular lipid was not produced in high quantities. Maximum total citric acid up to 49 g/L (yield 0.85 g/g glucose) was produced. In some of the wild-type strains, an increase of glucose in the medium also induced noticeable production of acetic acid. Increasing the amount of added glucose led to an increase in the total lipid quantity (%) produced, although in the stationary growth phase the concentration of lipid declined, indicating lipid degradation even for the genetically modified strains. Total lipid amount did not exceed the value of 14%, while neutral fractions increased with increase in glucose concentration. In all cases, the total microbial lipids and major lipid fractions were composed of C16 and C18 (principally unsaturated) fatty acids. Finally, in several of the strains cultured in media containing a low glucose concentration, unicellular morphology was observed, while at high glucose concentrations mycelia were predominant.read more
Citations
More filters
Journal ArticleDOI
Lipids of oleaginous yeasts. Part I: Biochemistry of single cell oil production.
TL;DR: In the first part of this review, the biochemistry of lipid accumulation in the oleaginous microorganisms is depicted, as preferential degradation of the neutral lipid fractions is observed.
Journal ArticleDOI
Metabolic activities of biotechnological interest in Yarrowia lipolytica grown on glycerol in repeated batch cultures
TL;DR: This study allows concluding that Y. lipolytica successfully converts glycerol via phosphorylation pathway into valuable biotechnological products, such as single cell oil and citric acid.
Journal ArticleDOI
Bioconversion of volatile fatty acids into lipids by the oleaginous yeast Yarrowia lipolytica.
TL;DR: The study demonstrated the feasibility of simultaneous biovalorization of volatile fatty acids and glycerol, two cheap industrial by-products, and its composition was found quite similar to that of vegetable oils.
Journal ArticleDOI
Lipid production from Yarrowia lipolytica Po1g grown in sugarcane bagasse hydrolysate
TL;DR: This study investigated the possibility of utilizing detoxified sugarcane bagasse hydrolysate (DSCBH) as an alternative carbon source to culture Yarrowia lipolytica Po1g for microbial oil and biodiesel production.
Journal ArticleDOI
Biotechnological conversions of biodiesel derived waste glycerol by yeast and fungal species
Afroditi Chatzifragkou,Anna Makri,Aikaterini Belka,Stamatina Bellou,Marilena Mavrou,Maria Mastoridou,Paraskevi Mystrioti,Grace Onjaro,George Aggelis,Seraphim Papanikolaou +9 more
TL;DR: In this article, 15 eukaryotic microorganisms were tested for their ability to assimilate biodiesel derived waste glycerol and convert it into value-added metabolic products.
References
More filters
Journal ArticleDOI
Physiology and genetics of the dimorphic fungus Yarrowia lipolytica
Gerold Barth,Claude Gaillardin +1 more
TL;DR: This review presents a comprehensive overview on the available data on physiology, cell biology, molecular biology and genetics of Y. lipolytica.
Journal ArticleDOI
Lipid production by Yarrowia lipolytica growing on industrial glycerol in a single-stage continuous culture.
TL;DR: Yarrowia lipolytica LGAM S(7)1 presented remarkable growth on industrial glycerol used as sole carbon substrate whilst fat-free material yield increased over the whole range of D (h(-1)).
Journal ArticleDOI
Biotechnological valorisation of raw glycerol discharged after bio-diesel (fatty acid methyl esters) manufacturing process: Production of 1,3-propanediol, citric acid and single cell oil
Seraphim Papanikolaou,Seraphim Papanikolaou,Stylianos Fakas,Stylianos Fakas,Michel Fick,Isabelle Chevalot,Maria Galiotou-Panayotou,Michael Komaitis,Ivan Marc,George Aggelis +9 more
TL;DR: Fatty acid analysis of total cellular lipids showed that glycerol concentration increase in the growth medium somehow increased the cellular unsaturated fatty acids content of lipids.
Journal Article
New perspectives for citric acid production and application
TL;DR: The use of agro-industrial residues as support in solid-state fermentation is economically important and minimizes environmental problems as mentioned in this paper, which can be used in the production of citric acid.
Journal ArticleDOI
Yarrowia lipolytica as a potential producer of citric acid from raw glycerol
TL;DR: In this article, the biochemical response of Yarrowia lipolytica during growth on raw glycerol (the main byproduct of bio-diesel production units) in order to produce metabolic products of industrial significance was studied.
Related Papers (5)
The biochemistry and molecular biology of lipid accumulation in oleaginous microorganisms
Colin Ratledge,James P. Wynn +1 more