Journal ArticleDOI
Carboxylated multiwalled carbon nanotubes based biosensor for aflatoxin detection
Chandan Singh,Saurabh Srivastava,Saurabh Srivastava,Md. Azahar Ali,Tejendra K. Gupta,Gajjala Sumana,Anchal Srivastava,Rakesh B. Mathur,Bansi D. Malhotra,Bansi D. Malhotra +9 more
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In this article, carboxylated multiwalled carbon nanotubes (c-MWCNTs) electrophoretically deposited onto indium tin oxide (ITO) glass have been functionalized with monoclonal aflatoxin B1 antibodies (anti-AFB1).Abstract:
We report results of studies relating to the development of an electrochemical immunosensor based on carboxylated multiwalled carbon nanotubes (c-MWCNTs) electrophoretically deposited onto indium tin oxide (ITO) glass. This c-MWCNTs/ITO electrode surface has been functionalized with monoclonal aflatoxin B1 antibodies (anti-AFB1) for the detection of aflatoxin-B1 using electrochemical technique. Electron microscopy, X-ray diffraction and Raman studies suggest successful synthesis of c-MWCNTs and the Fourier transform infra-red spectroscopic (FT-IR) studies reveal its carboxylic functionalized nature. The proposed immunosensor shows high sensitivity (95.2 μA ng−1 mL cm−2), improved detection limit (0.08 ng mL−1) in the linear detection range of 0.25–1.375 ng mL−1. The low value of association constant (0.0915 ng mL−1) indicates high affinity of immunoelectrode towards aflatoxin (AFB1).read more
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Nanoparticle-Based Immunochemical Biosensors and Assays: Recent Advances and Challenges
TL;DR: This work reviews the progress achieved during the recent five years in immunochemical biosensors (immunosensors) combined with nanoparticles for enhanced sensitivity and introduces antibodies as classic recognition elements.
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Electrochemical biosensors: perspective on functional nanomaterials for on-site analysis.
TL;DR: Although several functional nanomaterials have provided the innovative solid substrate for high performances, developing on-site version of biosensor that meets enough sensitivity along with high reproducibility still remains a challenge.
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Review—Recent Advances in Carbon Nanomaterials as Electrochemical Biosensors
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Analytical methods for determination of mycotoxins: An update (2009–2014)
Nicholas W. Turner,Heli Bramhmbhatt,Monika Szabo-Vezse,Alessandro Poma,R. D. Coker,Sergey A. Piletsky +5 more
TL;DR: This review summarises traditional methods such as chromatographic and immunochemical techniques, as well as newer approaches such as biosensors, and optical techniques which are becoming more prevalent, which are being more prevalent in the field of mycotoxins.
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Non-Covalent Functionalization of Carbon Nanotubes for Electrochemical Biosensor Development.
TL;DR: This review provides a comprehensive review of non-covalent functionalization of carbon nanotubes with a variety of biomolecules for the development of electrochemical biosensors and finds this method of immobilization is increasingly being used in bioelectrode development using enzymes for biosensor and biofuel cell applications.
References
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Book
Official Methods of Analysis of AOAC International
William Horwitz,George W Latimer +1 more
TL;DR: In this article, the authors present official methods of analysis of AOAC International, official methods for analysis of aOAC-related project, and a set of methods for the analysis of their work.
Journal ArticleDOI
Chemical oxidation of multiwalled carbon nanotubes
Vitaliy Datsyuk,M. Kalyva,Konstantinos Papagelis,John Parthenios,Dimitrios Tasis,Angeliki Siokou,Ioannis Kallitsis,Costas Galiotis +7 more
TL;DR: In this article, the effect of oxidation on the structural integrity of multiwalled carbon nanotubes through acidic (nitric acid and a mixture of sulfuric acid and hydrogen peroxide) and basic (ammonium hydroxide/hydrogen peroxide), agents has been studied.
Journal ArticleDOI
Estimates of the worldwide incidence of 25 major cancers in 1990
TL;DR: There are large differences in the relative frequency of different cancers by world area, and tobacco smoking and chewing are almost certainly the major preventable causes of cancer today.
Journal ArticleDOI
Low-potential stable NADH detection at carbon-nanotube-modified glassy carbon electrodes
TL;DR: Carbon-nanotube (CNT)-modified glassy carbon electrodes exhibiting strong and stable electrocatalytic response toward NADH are described in this article, where a substantial (490 mV) decrease in the overvoltage of the NADH oxidation reaction (compared to ordinary carbon electrodes) is observed using single-wall and multi-wall carbon nanotube coatings, with oxidation starting at ca. − 0.05 V (vs. Ag/AgCl; pH 7.4).
Journal ArticleDOI
Protein electrochemistry using aligned carbon nanotube arrays.
J. Justin Gooding,Rahmat Wibowo,Jingquan Liu,Wenrong Yang,Dusan Losic,Shannon Orbons,Freya J. Mearns,Joseph G. Shapter,D. Brynn Hibbert +8 more
TL;DR: In this paper, the authors showed that carbon nanotubes can be aligned normal to an electrode by self-assembly and act as molecular wires to allow electrical communication between the underlying electrode and redox proteins covalently attached to the ends of the SWNTs.