Journal ArticleDOI
Cell-penetrating peptides for the delivery of nucleic acids
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TLDR
This review will discuss recent advances of CPP-mediated delivery of nucleic acid-based cargo, concentrating on the delivery of plasmid DNA, splice-correcting ONs, and small-interfering RNAs.Abstract:
Introduction: Different gene therapy approaches have gained extensive interest lately and, after many initial hurdles, several promising approaches have reached to the clinics. Successful implementation of gene therapy is heavily relying on finding efficient measures to deliver genetic material to cells. Recently, non-viral delivery of nucleic acids and their analogs has gained significant interest. Among non-viral vectors, cell-penetrating peptides (CPPs) have been extensively used for the delivery of nucleic acids both in vitro and in vivo. Areas covered: In this review we will discuss recent advances of CPP-mediated delivery of nucleic acid-based cargo, concentrating on the delivery of plasmid DNA, splice-correcting ONs, and small-interfering RNAs. Expert opinion: CPPs have proved their potential as carriers for nucleic acids. However, similarly to other non-viral vectors, CPPs require further development, as efficient systemic delivery is still seldom achieved. To achieve this, CPPs should be modified...read more
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Journal ArticleDOI
Cell-penetrating peptides: 20 years later, where do we stand?
Cherine Bechara,Sandrine Sagan +1 more
TL;DR: This review is dedicated to CPP fundamentals, with an emphasis on the molecular requirements and mechanism of their entry into eukaryotic cells.
Journal ArticleDOI
Cell-Penetrating Peptides : Design, Synthesis, and Applications
TL;DR: The intrinsic property of cell-penetrating peptides to deliver therapeutic molecules to cells and tissues in a nontoxic manner has indicated that they may be potential components of future drugs and disease diagnostic agents.
Journal ArticleDOI
The delivery of therapeutic oligonucleotides.
TL;DR: A variety of current approaches for enhancing the delivery of oligonucleotides including molecular scale targeted ligand-oligonucleotide conjugates, lipid- and polymer-based nanoparticles, antibody conjugate and small molecules that improve oligon nucleotide delivery are examined.
Journal ArticleDOI
Non viral vectors in gene therapy- an overview.
TL;DR: This review will see in detail about various types of non-viral vectors highlighting promising development and recent advances that had improved the non-Viral gene transfer efficiency of translating from "Bench to bedside".
Journal ArticleDOI
Cellular Uptake of Nanoparticles versus Small Molecules: A Matter of Size.
TL;DR: Particular attention is paid to approaches that allow conditional regulation of the cell internalization process using external triggers, such as activable cell penetrating peptides, due to the impact these systems may have in drug delivery and sensing applications.
References
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Stearylated octaarginine and artificial virus-like particles for transfection of siRNA into primary rat neurons
TL;DR: Stearyl-R8 and AVPs may represent novel and more cost-efficient alternatives to conventional siRNA-transfection reagents and yield performances comparable to cationic liposome-mediated transfection for siRNA, while being less cytotoxic in primary neurons.
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HIV tat peptide enhances cellular delivery of antisense morpholino oligomers
TL;DR: HIV Tat peptide significantly enhances delivery of PMO in 100% of cells assayed and pTat-mediated delivery is a much simpler procedure to perform than other delivery methods.
Journal ArticleDOI
Direct Translocation as Major Cellular Uptake for CADY Self-Assembling Peptide-Based Nanoparticles
Anna Rydström,Sébastien Deshayes,Karidia Konate,Laurence Crombez,Kärt Padari,Hassan Boukhaddaoui,Gudrun Aldrian,Margus Pooga,Gilles Divita +8 more
TL;DR: Evidence is provided that the major route for CADY:siRNA cellular uptake involves direct translocation through the membrane but not the endosomal pathway, and it is proposed that direct translocated is the major gate for cell entry of CADY;siRNA complexes.
Journal ArticleDOI
A Peptide-based Vector for Efficient Gene Transfer In Vitro and In Vivo
Taavi Lehto,Taavi Lehto,Oscar E. Simonson,Imre Mäger,Imre Mäger,Kariem Ezzat,Helena Sork,Dana-Maria Copolovici,Joana R Viola,Eman M. Zaghloul,Per Lundin,Pedro Moreno,Maarja Mäe,Nikita Oskolkov,Julia Suhorutsenko,C. I. Edvard Smith,Samir El Andaloussi +16 more
TL;DR: Stearyl-TP10/plasmid nanoparticles are nonimmunogenic, mediate efficient gene delivery in vivo, when administrated intramuscularly ( i.m.) or intradermally (i.d.) without any associated toxicity in mice.
Journal ArticleDOI
Delivery of steric block morpholino oligomers by (R-X-R)4 peptides: structure–activity studies
Rachida Abes,Hong M. Moulton,Philippe Clair,Sung-Tae Yang,Saïd Abes,Kamran Melikov,Paul Prevot,Derek S. Youngblood,Patrick L. Iversen,Leonid V. Chernomordik,Bernard Lebleu +10 more
TL;DR: A significant correlation between splicing correction efficiency, affinity for heparin and ability to destabilize model synthetic vesicles has been observed and no correlation with cellular uptake has been found, suggesting endosomal escape remains the limiting factor for the delivery of these splice-redirecting ON analogs.